The effect of astaxanthin and cadmium on rat erythrocyte G6PD, 6PGD, GR, and TrxR enzymes activities in vivo and on rat erythrocyte 6PGD enzyme activity in vitro

Abstract

In this study, the effects of astaxanthin (AST) that belongs to carotenoid family and cadmium (Cd), which is an important heavy metal, on rat erythrocyte G6PD, 6PGD, GR, and TrxR enzyme activities in vivo and on rat erythrocyte 6PGD enzyme activity in vitro were studied. In in vitro studies, 6PGD enzyme was purified from rat erythrocytes with 2',5'-ADP Sepharose4B affinity chromatography. Results showed inhibition of enzyme by Cd at IC ; 346.5 μM value and increase of 6PGD enzyme activity by AST. In vivo studies showed an increase in G6PD, 6PGD, and GR enzyme activities (P ˃ 0.05) and no chance in TrxR enzyme activity by AST. Cd ion inhibited G6PD, 6PGD, and GR enzyme activities (P ˂ 0.05) and also decreased TrxR enzyme activity (P ˃ 0.05). AST + Cd group G6PD enzyme activity was statistically low compared with control group (P ˂ 0.05). 6PGD and TrxR enzyme activities decreased without statistical significance (P ˃ 0.05); however, GR enzyme activity increased statistically significantly (P ˂ 0.05).

Characterization of glutathioneS‐transferase enzyme from brown meagre (Sciaena umbra) and inhibitory effects of heavy metals

“…In many studies, the reduction of induced phase I/phase II activities; shows that it can result in many diseases or, moreover, death [28][29][30][31][32][33][34]. Therefore, inhibition studies on GST are extremely important researches.…”

Sıçan Eritrositlerinde Karbon Tetraklorürün(CCl4) ve Kuersetinin Bazı Metabolik Enzim Aktiviteleri Üzerine İn Vivo Etkisinin İncelenmesi

“…Samples were centrifuged at +4°C (10000 xg) for 30 minutes to eliminated cell membranes and insoluble molecules. The supernatant was saved for analysis [33].…”

Koyun Dalak Dokusundan Saflaştırılan Glukoz-6-Fosfat Dehidrogenaz ve Glutatyon Redüktaz Enzimi Üzerine Diklofenak Sodyumun İn Vitro Etkileri

“…Sıçanlarla yapılan bir çalışmada süperoksit dismutaz (SOD) ve katalaz (CAT) enzimlerinin diklofenak tarafından inhibe edildiği ve GSH miktarının önemli derecede azaldığı tespit edilmiştir (Zhu and Zhang, 2012). Farelerle yapılan diğer bir çalışmada da 50 mg kg -1 dozundaki diklofenak sodyumun P450 redüktaz enzim aktivitelerinde çok önemli derecede inhibisyona neden olduğu tespit edilmiştir (Bayramoğlu Akkoyun et al, 2018). GR enziminin insan eritrosit hücrelerinden saflaştırıldığı bir çalışmada enzim üzerine bazı analjezik ve anastetik ilaçların etkileri araştırılmıştır.…”