The effect of burdock leaf fraction on adhesion, biofilm formation, quorum sensing and virulence factors of<i>Pseudomonas aeruginosa</i>

Lou, Zaixiang; Wang, H.; Tang, Yuxia; Chen, X.

doi:10.1111/jam.13348

Cited by 15 publications

(6 citation statements)

References 57 publications

(87 reference statements)

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“…Present results showed that garlic extracts, nisin, ε-polylysine, and citric acid decreased the growth, EPS production, bacterial surface hydrophobicity, and biofilm formtion of B. subtilis. This is in agreement with previous studies which found that bacterial biofilm and the adhesion rate on surfaces can be reduced by reducing the production of EPS with antibacterial substance (Branda et al, 2006;Lou, Wang, & Tang, 2017;Rathi & Satheesh, 2012).…”

Section: Re Sults and Discussionsupporting

confidence: 93%

Comparative antibacterial and antibiofilm activities of garlic extracts, nisin, ε‐polylysine, and citric acid on Bacillus subtilis

Zhang

Cai

et al. 2019

J Food Process Preserv

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Bacillus subtilis shows high contamination and antibiotic resistance in the food industry due to their biofilm‐forming capacity. This study aimed to search a biopreservative to inhibit the biofilm formation of B. subtilis. The effect of garlic extracts, nisin, ε‐polylysine, and citric acid on bacterial growth, cell surface hydrophobicity, and cell morphology were observed to provide insight into the antibiofilm mechanisms. Results showed that nisin, ε‐polylysine, and citric acid completely inhibited the growth and biofilm formation of B. subtilis at 0.15, 0.09, and 1.5 mg/ml, respectively. Garlic extracts inhibited cell elongation, but cannot completely inhibit the biofilm formation even at 1.8 mg/ml. The antibiofilm activities of four biopreservatives were due to antibacterial activities, reduction in extracellular polymeric substances production, and cell surface hydrophobicity. Among these four biopreservatives, ε‐polylysine, which caused cell to collapse, had the highest antibacterial and antibiofilm activities followed by nisin, citric acid, and garlic extracts. Practical applications Bacillus subtilis shows high contamination and antibiotic resistance due to their biofilm‐forming capacity. Finding a biopreservative to inhibit the growth and biofilm formation of B. subtilis was important in food industry. This study could pave the way for controlling B. subtilis in planktonic and biofilm states.

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Section: Re Sults and Discussionsupporting

confidence: 93%

Comparative antibacterial and antibiofilm activities of garlic extracts, nisin, ε‐polylysine, and citric acid on Bacillus subtilis

Zhang

Cai

et al. 2019

J Food Process Preserv

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“…Quorum Sensing regulates various bacterial behaviors, such as the formation of biofilm and the secretion of virulence factors (such as elastase, rhamnolipid, and pyocyanin; Lou et al, 2017). QS inhibitors are able to interfere with the QS system of pathogens and reduce the pathogenic effects, However, the majority of QS inhibitors have been found to be toxic, and cannot be used in medicine fields.…”

Section: Anti-qs Activitymentioning

confidence: 99%

Novel Opportunity to Reverse Antibiotic Resistance: To Explore Traditional Chinese Medicine With Potential Activity Against Antibiotics-Resistance Bacteria

Qiu

Hua

et al. 2020

Front. Microbiol.

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Antibiotic resistance is becoming significantly prominent and urgent in clinical practice with the increasing and wide application of antibacterial drugs. However, developing and synthesizing new antimicrobial drugs is costly and time-consuming. Recently, researchers shifted their sights to traditional Chinese medicine (TCM). Here, we summarized the inhibitory mechanism of TCM herbs and their active ingredients on bacteria, discussed the regulatory mechanism of TCM on antibiotic-resistant bacteria, and revealed preclinical results of TCM herbs and their active components against antibiotic-resistant bacteria in mouse models. Those data suggest that TCM herbs and their effective constituents exhibit potential blockage ability on antibiotic-resistant bacteria, providing novel therapeutic ideas for reversing antibiotic resistance.

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“…The method with reference to Lou et al was slightly modified [ 22 ]. Briefly, 100 μL of supernatant was obtained by centrifuging the bacterial solution (the same as item 2.8), extracted twice by adding ethyl acetate, evaporated at 40 °C, dissolved by adding 100 μL of ultrapure water, reacted by adding 300 μL of a 2% anthrone-sulfuric acid solution in boiling water for 15 min, and cooled.…”

Section: Methodsmentioning

confidence: 99%

Screening and Identification of a Streptomyces Strain with Quorum-Sensing Inhibitory Activity and Effect of the Crude Extracts on Virulence Factors of Pseudomonas aeruginosa

Zhang

Sun

et al. 2023

Microorganisms

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Quorum-sensing (QS) is involved in numerous physiological processes in bacteria, such as biofilm formation, sporulation, and virulence formation. Therefore, the search for new quorum-sensing inhibitors (QSI) is a promising strategy that opens up a new perspective for controlling QS-mediated bacterial pathogens. To explore new QSIs, a strain named Streptomyces sp. D67 with QS inhibitory activity was isolated from the soil of the arid zone around the Kumutag Desert in Xinjiang. Phylogenetic analyses demonstrated that strain D67 shared the highest similarity with Streptomyces ardesiacus NBRC 15402T (98.39%), which indicated it represented a potential novel species in the Streptomyces genus. The fermentation crude extracts of strain D67 can effectively reduce the violacein production produced by Chromobacterium violaceum CV026 and the swarming and swimming abilities of Pseudomonas aeruginosa. It also has significant inhibitory activity on the production of virulence factors such as biofilm, pyocyanin, and rhamnolipids of P. aeruginosa in a significant concentration-dependent manner, but not on protease activity. A total of 618 compounds were identified from the fermentation crude extracts of strain D67 by LC-MS, and 19 compounds with significant QS inhibitory activity were observed. Overall, the strain with QS inhibitory activity was screened from Kumutag Desert in Xinjiang for the first time, which provided a basis for further research and development of new QSI.

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The effect of burdock leaf fraction on adhesion, biofilm formation, quorum sensing and virulence factors ofPseudomonas aeruginosa

Abstract: This study introduces a natural and effective bacterial biofilm inhibitor, which could also significantly decrease the content of virulence factors and the drug resistance of P. aeruginosa.

Cited by 15 publications

References 57 publications

Comparative antibacterial and antibiofilm activities of garlic extracts, nisin, ε‐polylysine, and citric acid on Bacillus subtilis

Comparative antibacterial and antibiofilm activities of garlic extracts, nisin, ε‐polylysine, and citric acid on Bacillus subtilis

Novel Opportunity to Reverse Antibiotic Resistance: To Explore Traditional Chinese Medicine With Potential Activity Against Antibiotics-Resistance Bacteria

Screening and Identification of a Streptomyces Strain with Quorum-Sensing Inhibitory Activity and Effect of the Crude Extracts on Virulence Factors of Pseudomonas aeruginosa

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