Introduction Rolling resistance (RR) is a drag force acting on manual wheelchairs that is associated with increased propulsion force and is linked to secondary disabling conditions of the upper limbs. A scoping review was conducted to understand how RR of manual wheelchairs has been measured and to identify limitations of those test methods and the factors tested. Methods A total of 42 papers were identified and reviewed, and test methods were categorized based on the measurement style of RR, testing level, and if multiple parameters could be tested. Additionally, 34 articles were reviewed for what factors were tested. Results Seven different testing methods categories were identified: drag test, treadmill, motor draw, deceleration, physiological expenditure, ergometer/dynamometer, and robotic test rig. Relevant articles were categorized into testing factor categories: camber, toe, tire type, tire pressure, caster type, mass, mass distribution, and type of surface. Conclusions The variety of testing methods suggests the need for a standardized method that can be used for wheelchair wheel design and selection to reduce RR. It is important to use adjustments, such as a forward rear axle position to mitigate RR as well as using high-pressure pneumatic tires that are properly inflated.