The effect of cavity restoration variables on odontoblast cell numbers and dental repair

Early Odontoblastic Layer Response to Cavity Preparation and Acid Etching in Rats

“…The long-term success of a dental procedure depends on preserving the odontoblasts vitality and functioning, therefore on preserving the natural dental pulp's repair mechanisms (ABOUT et al 2001). In this study, two factors influencing the vitality of odontoblasts were examined, i.e.…”

Pulpal Responses to Caries and Dental Repair

“…Cox et al [1992] have concluded that the effects of the surgical procedures during restoration may be greater than those of the restorative materials placed and such surgical damage is highlighted by the induction of apoptosis in odontoblasts after cavity preparation in rat molars [Kitamura et al, 2001]. The effects of restoration variables have been experimentally modelled [Murray et al, 2000b[Murray et al, , 2002a, and the possibility of pulpal cell death beneath cavity preparations is well recognised [Ohshima, 1990;Bronckers et al, 1996;Vermelin et al, 1996;About et al, 2001a]. This emphasises the need to discriminate between cellular injury and other responses directly arising 226 Caries Res 2002;36:223-232 Smith from the disease process and effects arising from subsequent restoration of the lesion.…”

“…Whilst no data are available on its survival and diffusion distance in caries lesions, data on dentine repair and odontoblast survival beneath restored cavity preparations and the influence of residual dentine thickness provide some indication. Residual dentine thickness is a key factor in dentine repair and odontoblast survival following cavity preparation and a residual dentine thickness of 0.25-0.5 mm appears to be optimal for dentine repair whilst minimising loss of odontoblast survival [About et al, 2001a;Murray et al, 2001]. This may imply that the distance over which growth factors can diffuse and maintain their activity is limited, although clearly many factors will influence this and in a caries situation there may be a greater and more sustained wave of growth factors released by the ongoing demineralisation process than is seen following cavity preparation.…”

Caracterización Estereológica de Odontoblastos en Pulpas Dentarias Humanas Sanas y con Pulpitis Reversible

“…Los odontoblastos son células altamente diferenciadas responsables de regular la síntesis, secreción y mineralización de la dentina durante la vida (Couble et al, 2000); estas han demostrado detectar y responder ante una injuria que afecta a la dentina incrementando su espesor, y forma una barrera entre los odontoblastos y el estímulo, función que depende de la capa odontoblástica (Brännström & Lind, 1965;Bjorndal & Darvann, 1999;Murray et al, 2001). La reacción y reparación dentinaria depende de diversos parámetros, incluyendo el número de odontoblastos (Murray et al, 2000;About et al, 2001).…”

“…La reacción y reparación dentinaria depende de diversos parámetros, incluyendo el número de odontoblastos (Murray et al, 2000;About et al, 2001). Otros fenotipos celulares de la pulpa dentaria, ubicados en la capa subodontoblástica y región central soportan a la dentinogénesis, pero no juegan un rol directo en la secreción de matriz dentinaria (Linde, 1985;Tziafas, 2010).…”