The effect of centrifugal force on mRNA levels of collagenase, collagen type‐I, tissue inhibitors of metalloproteinases and β‐actin in cultured human periodontal ligament fibroblasts

Redlich, Meir; Roos, H.; Reichenberg, E.; Zaks, Batia; Grosskop, A.; Kana, I. Bar; Pitaru, S.; Palmon, Aaron

doi:10.1111/j.1600-0765.2004.00700.x

Cited by 54 publications

(49 citation statements)

References 27 publications

(33 reference statements)

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“…where P = kg pressure per cm 2 of cells, m = mass of medium (0.002 kg), r = radius (0.09 m), rpm = revolution /min (600 or 4,800) and A = area of contact between medium and cells (9.62 cm 2 ) (20)(21)(22)(23). The cells on the dishes were then cultured continuously up to 7 days.…”

Section: Procedures Of Treating Cells With Centrifugal Forcementioning

confidence: 99%

Mechanical force promotes proliferation and early differentiation of bone marrow derived osteoblast-like cells in vitro

et al. 2009

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Abstract:The aim of this study was to examine the effect of centrifugal force on rat bone marrow osteoblast-like cells (RBM) in vitro. RBM were seeded and cultured for 2 days. Centrifugal forces were added to the cells in the culture dishes using a centrifuge for 20 min either at 600 rpm or 4,800 rpm. The cells without centrifugation were used as control. The expression of HSP27 mRNA of both experimental groups after addition of centrifugal force for 3 hrs was significantly up-regulated compared with the control group. In the 4,800 rpm groups, the cells showed contracted shapes compared to the controls and the 600 rpm groups after treatment with centrifugal force. The maximum height of each cell in the control group was 2.00 ± 0.33 μm. The maximum height of each cell in the 600 rpm group was 1.50 ± 0.23 μm, and in the 4,800 rpm group it was 1.13 ± 0.23 μm. The proliferation ratio in the 4,800 rpm group at day 3 was significantly higher than in the other group. The expression of type I collagen and alkaline phosphatase (ALP) mRNAs in both experimental groups increased at earlier time periods compared to the control groups. ALP activity revealed no significant difference between the experimental groups and the control group. A centrifugal force of 4,800 rpm might promote the proliferation and early differentiation of rat bone marrow cells.

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Section: Procedures Of Treating Cells With Centrifugal Forcementioning

confidence: 99%

Mechanical force promotes proliferation and early differentiation of bone marrow derived osteoblast-like cells in vitro

et al. 2009

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“…However, the expression patterns of these molecules in response differed according to the modes of the mechanical stress, such as shear, compression, and tension as well as the magnitudes of the stress (Redlich et al, 2004;Ozaki et al, 2005;Garlet et al, 2007;Kook et al, 2009). Previous evidence has shown that cyclic tensile stress (CTS) during orthodontic tooth movement (OTM) increases the expression of osteogenic genes and proteins such as alkaline phosphatase (ALP), secreted phosphoprotein 1 (SPP1; also known as bone sialoprotein I/osteopontin), transcription factor Sp7 (SP7; also known as osterix), and runt-related transcription factor 2 (RUNX2) of hPDLCs (Wescott et al, 2007;Cho et al, 2010;Tang et al, 2012).…”

mentioning

confidence: 99%

Cyclic Tensile Stress During Physiological Occlusal Force Enhances Osteogenic Differentiation of Human Periodontal Ligament Cells via ERK1/2-Elk1 MAPK Pathway

Han

Sheng

et al. 2013

DNA and Cell Biology

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Physiological occlusal force constitutively exists in the oral environment and is important for periodontal homeostasis and remodeling. Cyclic tensile stress (CTS) triggers the biological response of periodontal ligament (PDL). However, a few reports have studied the correlation between CTS during physiological occlusal force and PDL cell activities such as osteogenic differentiation. In the present study, human PDL cells (hPDLCs) were subjected to 10% elongation CTS loading at 0.5 Hz for 24 h, which represents the physiological conditions of occlusal force. Gene expression microarray was used to investigate the mechano-induced differential gene profile and pathway analysis in vitro. The osteogenic relative factors, that is, SPP1, RUNX2, and SP7, were assessed by real-time PCR and Western blot. The involvement of mitogen-activated protein kinase (MAPK) signaling pathways was investigated by Western blot with a specific inhibitor. The expressions of SPP1, RUNX2, SP7, p-ERK1/2, and p-Elk1 were up-regulated after 10% CTS exposure. However, these up-regulated expressions were prevented by ERK1/2 inhibitor U0126 in the physiological occlusal force-applied hPDLCs. These results showed that 10% CTS could enhance osteogenic differentiation of hPDLCs via ERK1/2-Elk1 MAPK pathway, indicating that CTS during physiological occlusal force is a potent agent for PDL remodeling.

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“…We reported that continuous force induced morphological changes of cultured osteoblasts and possibly affected the autocrine structural pathway that might link the nucleus to the mechanotransduction and accelerate the expression of the c-fos gene in the nucleus (30). OPN has been recognized as a regulator of responses to mechanical stress (21,26,37). Toma et al demonstrated that OPN produced by osteoblasts plays a role in the mechanosignal transduction, and this possibly depends on a protein kinase A-like kinase (36).…”

Section: Discussionmentioning

confidence: 99%

“…The effects of various types of mechanical stress, such as centrifugal force (23)(24)(25)(26)(27), stretching force (11,17,20,(28)(29), compressive force (30) and shear stress (18) on cells have been reported. An advantage of using centrifugal force in vitro is that it is possible to add even amounts to cells without changing the culture conditions, such as decreasing the oxygen or depleting the nourishment.…”

Section: Discussionmentioning

confidence: 99%

Effect of mechanical loading on cultured osteogenic cells derived from different stages of bone wound healing in rats: experimental models for immediate or early implantation

et al. 2010

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Abstract:The purpose of this study was to evaluate the effects of mechanical loading on cultured osteogenic cells from different stages of bone wound healing. A bone cavity was created in a rat femur, and cells were obtained from the cavity during various phases of wound healing: the blood clot (BC) stage, the development of granulation tissue (GT), and callus formation (CF). After 7 days of primary culture, mechanical loading was applied once a day. Cells without mechanical loading were used as controls. Cell proliferation, alkaline phosphatase (ALP) activity and expression of mRNAs of both bone sialoprotein (BSP) and osteopontin (OPN) were evaluated. Cell proliferation of BC cells was significantly inhibited by mechanical loading. However, GT cells were significantly up-regulated by ALP activity at day 5, as were the expressions of BSP and OPN mRNAs at day 7 by mechanical loading. These results suggest that cells in bone granulation tissues have the ability to transduce mechanical stress to osteogenic differentiation.

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The effect of centrifugal force on mRNA levels of collagenase, collagen type‐I, tissue inhibitors of metalloproteinases and β‐actin in cultured human periodontal ligament fibroblasts

Abstract: The results indicate that PDL remodeling following application of orthodontic force could be partly attributed to the direct effect of the force on MMP-1 gene expression in fibroblasts.

Cited by 54 publications

References 27 publications

Mechanical force promotes proliferation and early differentiation of bone marrow derived osteoblast-like cells in vitro

Mechanical force promotes proliferation and early differentiation of bone marrow derived osteoblast-like cells in vitro

Cyclic Tensile Stress During Physiological Occlusal Force Enhances Osteogenic Differentiation of Human Periodontal Ligament Cells via ERK1/2-Elk1 MAPK Pathway

Effect of mechanical loading on cultured osteogenic cells derived from different stages of bone wound healing in rats: experimental models for immediate or early implantation

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