The effect of changes in salinity on the energy yielding processes of Chlorella vulgaris and Dunaliella maritima cells

Alyabyev, A.Ju.; Loseva, N.L.; Gordon, L. Kh.; Andreyeva, I.N.; Rachimova, G.G.; Tribunskih, V.I.; Пономарева, А. А.; Кемп, Р. Б.

doi:10.1016/j.tca.2007.03.003

Cited by 58 publications

(22 citation statements)

References 20 publications

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“…Chlorella is known to be tolerant to salinity stress (Alyabyev et al. ) and one of the strains isolated in this study was tolerant to very high salinity levels (B‐H.K., D‐H.C., H‐M.O., and H‐S.K., unpublished data).…”

Section: Resultssupporting

confidence: 53%

Novel approach for the development of axenic microalgal cultures from environmental samples

Cho

Ramanan

Kim

et al. 2013

Journal of Phycology

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We demonstrated a comprehensive approach for development of axenic cultures of microalgae from environmental samples. A combination of ultrasonication, fluorescence-activated cell sorting (FACS), and micropicking was used to isolate axenic cultures of Chlorella vulgaris Beyerinck (Beijerinck) and Chlorella sorokiniana Shihira & R.W. Krauss from swine wastewater, and Scenedesmus sp. YC001 from an open pond. Ultrasonication dispersed microorganisms attached to microalgae and reduced the bacterial population by 70%, and when followed by cell sorting yielded 99.5% pure microalgal strains. The strains were rendered axenic by the novel method of micropicking and were tested for purity in both solid and liquid media under different trophic states. Denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene confirmed the absence of unculturable bacteria, whereas fluorescence microscopy and scanning electron microscopy (SEM) further confirmed the axenicity. This is the most comprehensive approach developed to date for obtaining axenic microalgal strains without the use of antibiotics and repetitive subculturing.

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Section: Resultssupporting

confidence: 53%

Novel approach for the development of axenic microalgal cultures from environmental samples

Cho

Ramanan

Kim

et al. 2013

Journal of Phycology

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“…According to Alyabyev et al (2007), salt stress causes a series of bioenergetic and biochemical changes in photosynthetic organisms. Among them, the most important are the increase in biopolymer and lipid catabolism, changes in the energy rate that supports biochemical processes, and changes in the permeability of the plasma membrane with disruption of ion homeostasis.…”

Section: Introductionmentioning

confidence: 99%

Lipid content of marine microalgae Chaetoceros muelleri Lemmermann (Bacillariophyceae) grown at different salinities

et al. 2014

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ResumoTeor lipídico da microalga marinha Chaetoceros muelleri Lemmermann (Bacillariophyceae) cultivada em diferentes salinidades. A produção e armazenamento de lipídios por microalgas em resposta a variações nos fatores ambientais são específicos para cada espécie de microalga. O presente trabalho teve como objetivo avaliar o crescimento e a concentração de lipídios totais da microalga Chaetoceros muelleri cultivada em três diferentes salinidades (15, 25 e 35). O experimento foi realizado em triplicatas, utilizando frascos de nove litros com volumes úteis de 7 L, sendo cultivo do tipo estacionário ou "batch". A temperatura da sala de cultivo e a intensidade luminosa foram mantidas em 28±1ºC e 20 μ.mol.m -2 .s -1 , respectivamente. Os resultados mostraram que no presente trabalho, o rendimento lipídico (10,41±1,89, 10,87±2,10, 12,33±1,81% nas salinidades 15, 25 e 35, respectivamente) não foi influenciado pela concentração salina (p>0,05). No entanto, a concentração celular máxima sofreu esta influência e foi significativamente maior (p<0,05) nas salinidades de 15 e 25 as quais obtiveram 16,3±1,50 e 17,6±2,90 x 10 5 cel.mL -1 , respectivamente. Key words: Cultivo; Lipídeos; Microalgas AbstractThe production and storage of lipids by microalgae in response to variations in environmental factors are specific for each microalgal species. The present study aimed to evaluate the growth and concentration of total lipids of the microalga C. muelleri Lemmermann cultivated at three different salinities (15, 25 and 35). The experiment was conducted in triplicate, using 9-L containers (7-L working volume), with the culture being stationary or batch type. The temperature and light intensity of the cultivation room was kept at 28±1ºC and 20 μ.mol.

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“…There are many nutritional and environmental factors controlling the cell growth and its lipid content, such as organic and inorganic carbon sources [28,29], nitrogen source [30][31][32], and other essential macro-and micro-nutrients like magnesium and copper [33,34], temperature, pH level, salinity, agitation speed (dissolved oxygen), etc. [35][36][37]. Since C/N ratio is generally considered to influence the cellular lipid content in the cells [38][39][40], carbon source and concentration, and nitrogen concentration were examined in this study, along with the salinity, pH level, and agitation speed of the cultures.…”

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confidence: 99%

Oil Accumulation via Heterotrophic/Mixotrophic Chlorella protothecoides

Heredia-Arroyo

Wei

2010

Appl Biochem Biotechnol

211

107

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Microalgal oil is a potential energy source because it can be easily converted to fatty acid methyl ester or hydrocarbon type of diesel, and it is produced with relatively higher productivity compared with oil from plants and animals. Heterotrophic growth of microalgae is superior due to its high final product concentration; however, the cost of the raw materials is unacceptable if sugar is utilized as the carbon source. The aim of this study is to optimize the lipid accumulation of Chlorella protothecoides by using carbon sources other than glucose in heterotrophic and mixotrophic cultures. Different factors such as different carbon sources, carbon to nitrogen ratio, initial pH level, salinity, and rotational speed are studied in affecting the cell growth and the oil accumulation. Our experiments revealed that the heterotrophic and mixotrophic cultures of C. protothecoides grew better than autotrophic cultures. C. protothecoides can grow on glycerol or acetate, as well as on glucose. Several stress factors were confirmed or discovered to significantly increase the lipid content of microalgae cells. The replacement of glycerol and acetate as carbon sources for microalgae cultivations provides potential for waste utilization: glycerol from biodiesel industry and acetate from biohydrogen production.

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The effect of changes in salinity on the energy yielding processes of Chlorella vulgaris and Dunaliella maritima cells

Cited by 58 publications

References 20 publications

Novel approach for the development of axenic microalgal cultures from environmental samples

Novel approach for the development of axenic microalgal cultures from environmental samples

Lipid content of marine microalgae Chaetoceros muelleri Lemmermann (Bacillariophyceae) grown at different salinities

Oil Accumulation via Heterotrophic/Mixotrophic Chlorella protothecoides

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