The effect of cholesterol levels on hematopoietic stem cell mobilization

Dönmez, Ayhan; Kabaroğlu, Ceyda; Arik, Bahar; Tombuloğlu, Murat

doi:10.1016/j.transci.2012.03.009

Cited by 5 publications

(5 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A linear relationship was also found between peripheral blood CD34 + cell count and collected CD34 + cell amount after purification [ 4 , 5 ]. In general, the cutoff point of peripheral blood CD34 + cell count ranging from less than 10/μL to 50/μL has been set as a threshold to define poor mobilizers [ 5 , 14 , 15 , 31 , 32 , 33 , 34 , 35 , 36 ]. In the present study, we defined poor mobilizers as having CD34 ≤ 25/μL, a threshold that is within the reported values.…”

Section: Discussionmentioning

confidence: 99%

“…A linear relationship was also found between peripheral blood CD34 + cell count and collected CD34 + cell amount after purification [4,5]. In general, the cutoff point of peripheral blood CD34 + cell count ranging from less than 10/µL to 50/µL has been set as a threshold to define poor mobilizers [5,14,15,[31][32][33][34][35][36].…”

Section: Discussionmentioning

confidence: 99%

“…High-cholesterol diets can enhance HSC mobilization from bone marrow to the peripheral blood in mice [ 11 ], and cholesterol and low-density lipoprotein cholesterol may serve as key biomarkers for G-CSF-triggered HSC mobilization in humans [ 12 , 13 ]. However, one study demonstrated that the influence of cholesterol level on HSC mobilization is negligible in humans [ 14 ]. Furthermore, several single nucleotide polymorphisms of the genes involved in hematopoiesis and cell migration are associated with G-CSF-triggered HSC mobilization [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Correlation of Body Mass Index and Proinflammatory Cytokine Levels with Hematopoietic Stem Cell Mobilization

Wang

Liou

Chang

et al. 2022

JCM

View full text Add to dashboard Cite

This study investigated the correlation of body mass index (BMI) and proinflammatory cytokine levels with hematopoietic stem cell (HSC) mobilization triggered by granulocyte colony-stimulating factor (G-CSF). Stem cell donors (n = 309) were recruited between August 2015 and January 2018 and grouped into four groups according to their BMI: underweight (BMI < 18.5 kg/m2, n = 10), normal (18.5 kg/m2 ≦ BMI < 25 kg/m2, n = 156), overweight (25 kg/m2 ≦ BMI < 30 kg/m2, n = 102), and obese (BMI ≧ 30 kg/m2, n = 41). The participants were then administered with five doses of G-CSF and categorized as good mobilizers (CD34 ≧ 180/μL, n = 15, 4.85%) and poor mobilizers (CD34 ≦ 25/μL, n = 14, 4.53%) according to the number of CD34+ cells in their peripheral blood after G-CSF administration. The correlation between BMI and HSC mobilization was then analyzed, and the levels of proinflammatory cytokines in the plasma from good and poor mobilizers were examined by ProcartaPlex Immunoassay. Results showed that BMI was highly correlated with G-CSF-triggered HSC mobilization (R2 = 0.056, p < 0.0001). Compared with poor mobilizers, good mobilizers exhibited higher BMI (p < 0.001) and proinflammatory cytokine [interferon gamma (IFN-γ) (p < 0.05), interleukin-22 (IL-22) (p < 0.05), and tumor necrosis factor alpha (TNF-α) levels (p < 0.05)]. This study indicated that BMI and proinflammatory cytokine levels are positively correlated with G-CSF-triggered HSC mobilization.

show abstract

Section: Discussionmentioning

confidence: 99%

“…A linear relationship was also found between peripheral blood CD34 + cell count and collected CD34 + cell amount after purification [4,5]. In general, the cutoff point of peripheral blood CD34 + cell count ranging from less than 10/µL to 50/µL has been set as a threshold to define poor mobilizers [5,14,15,[31][32][33][34][35][36].…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Correlation of Body Mass Index and Proinflammatory Cytokine Levels with Hematopoietic Stem Cell Mobilization

Wang

Liou

Chang

et al. 2022

JCM

View full text Add to dashboard Cite

show abstract

“…Cholesterol also plays multiple roles in HSCs. Cholesterol efflux prevents HSPC mobilization and extra-medullary hematopoiesis [24] but does not affect the mobilization of hematopoietic stem cells [25]. CL mice have low circulating cholesterol level [26] although the potential impact on HSC repopulation remains to be studied.…”

Section: Discussionmentioning

confidence: 99%

Suppression of Cytochrome P450 Reductase Enhances Long-Term Hematopoietic Stem Cell Repopulation Efficiency in Mice

et al. 2013

View full text Add to dashboard Cite

BackgroundBone marrow microenvironment (niche) plays essential roles in the fate of hematopoietic stem cells (HSCs). Intracellular and extracellular redox metabolic microenvironment is one of the critical factors for the maintenance of the niche. Cytochrome P450 reductase (CPR) is an obligate electron donor to all microsomal cytochrome P450 enzymes (P450 or CYP), and contributes to the redox metabolic process. However, its role in maintaining HSCs is unknown.ObjectiveTo examine the effects of low CPR expression on HSCs function using a mouse model of globally suppressed Cpr gene expression (Cpr Low, CL mice).MethodsHematopoietic cell subpopulations in bone marrow (BM) and peripheral blood (PB) from WT and CL mice were examined for their repopulation and differentiation ability upon BM competitive transplantation and enriched HSC (LKS+) transplantation. Effects of low CPR expression on hematopoiesis were examined by transplanting normal BM cells into CL recipients. Reactive oxygen species (ROS), cell cycle, and apoptosis in CL mice were analyzed by flow cytometry for DCF-DA fluorescence intensity, Ki67 protein, and Annexin-V, respectively.ResultsThe levels of ROS in BM cells, HPCs and HSCs were comparable between CL and WT mice. In comparison to WT mice, the number of LT-HSCs or ST-HSCs was lower in CL mice while CMPs, GMPs and MEPs in CL mice were higher than that in WT control. Competitive transplantation assay revealed enhanced repopulation capacity of HSCs with low CPR expression, but no difference in differentiation potential upon in vitro experiments. Furthermore, lymphoid differentiation of donor cells decreased while their myeloid differentiation increased under CL microenvironment although the overall level of donor hematopoietic repopulation was not significantly altered.ConclusionsOur studies demonstrate that suppressing CPR expression enhances the repopulation efficiency of HSCs and a low CPR expression microenvironment favors the differentiation of myeloid over lymphoid lineage cells.

show abstract

“…These therapies/BM conditions are considered to cause damage to hematopoietic stem cells. Other factors that affect the collection efficiency include advanced age (>60 yr), >1 yr of treatment after diagnosis, low serum lactate dehydrogenase, and low serum cholesterol levels . However, almost all these studies were conducted on adults.…”

Section: Discussionmentioning

confidence: 99%

Delayed short‐term administration of granulocyte colony‐stimulating factor is a good mobilization strategy for harvesting autologous peripheral blood stem cells in pediatric patients with solid tumors

Takahashi

Kato

Kikuchi

et al. 2013

Pediatric Transplantation

View full text Add to dashboard Cite

PBSCs have become the preferred source of autologous stem cells for supporting high-dose chemotherapy in childhood solid tumors. The aims of this retrospective study were to examine the optimal timing for administration of G-CSF after chemotherapy and to identify the patients from whom an optimal dose of PBSCs can be harvested. We evaluated the timing of G-CSF administration for harvesting PBSCs in patients with childhood solid tumors. G-CSF was administered immediately after chemotherapy in eight patients (11 harvests, long-term group) and following recovery from hematological nadirs in 17 patients (21 harvests, short-term group). The median duration of G-CSF administration was 22 vs. 5 days, respectively (p < 0.005), and the dose of harvested CD34(+) cells (×10(6) /kg) was 1.4 vs. 2.9, respectively (p = 0.023). Our results suggest that short-term G-CSF administration is a good strategy for harvesting PBSCs in these patients.

show abstract

The effect of cholesterol levels on hematopoietic stem cell mobilization

Cited by 5 publications

References 8 publications

Correlation of Body Mass Index and Proinflammatory Cytokine Levels with Hematopoietic Stem Cell Mobilization

Correlation of Body Mass Index and Proinflammatory Cytokine Levels with Hematopoietic Stem Cell Mobilization

Suppression of Cytochrome P450 Reductase Enhances Long-Term Hematopoietic Stem Cell Repopulation Efficiency in Mice

Delayed short‐term administration of granulocyte colony‐stimulating factor is a good mobilization strategy for harvesting autologous peripheral blood stem cells in pediatric patients with solid tumors

Contact Info

Product

Resources

About