The effect of commercial conjugated linoleic acid products on experimental periodontitis and diabetes mellitus in Wistar rats

Yüce, Hatice Balcı; Akbulut, Nıhat; Ocaklı, Seda; Kayır, Ömer; Elmastaş, Mahfuz

doi:10.1080/00016357.2016.1244355

Cited by 20 publications

(10 citation statements)

References 67 publications

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“…Sample size in the study groups was determined based on a previous study with a similar study design. 30 All data were subjected to Kolmogorov-Smirnov test to test the normality. For the data with normal distribution, one-way ANOVA test followed by Tukey test was performed.…”

Section: Discussionmentioning

confidence: 99%

“…Cells do not have a cuboid shape, rather have a flat surface were considered as surface lining cells which are not active osteoblast producing osteoid and were not counted. Regarding the inflammatory cells, fibroblast cells and endothelial cells could easily be differentiated from inflammatory cells which were macrophage, neutrophil, eosinophil, T lymphocytes, and plasma cells . Fibroblasts have a thin and long spindle‐like shape, while other cells have a more rounded shape .…”

Section: Methodsmentioning

confidence: 99%

“…Statistical analysis was performed with IBM SPSS program (SPSS Inc.) (vs.20.00). Sample size in the study groups was determined based on a previous study with a similar study design . All data were subjected to Kolmogorov‐Smirnov test to test the normality.…”

Section: Methodsmentioning

confidence: 99%

“…Regarding the inflammatory cells, fibroblast cells and endothelial cells could easily be differentiated from inflammatory cells which were macrophage, neutrophil, eosinophil, T lymphocytes, and plasma cells. [28][29][30][31][32][33] Fibroblasts have a thin and long spindle-like shape, while other cells have a more rounded shape. 28,29,[31][32][33][34] The cells which were not easily differentiated were not counted.…”

Section: Histologic Evaluationmentioning

confidence: 99%

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Evaluation of the effect of oleuropein on alveolar bone loss, inflammation, and apoptosis in experimental periodontitis

Taşkan

Yüce

Karataş

et al. 2019

J of Periodontal Research

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The Objective The present study aimed to evaluate the effects of oleuropein on ligature‐induced alveolar bone loss. In this respect, osteoblastic activity, osteoclastic activity, inflammatory markers, and apoptosis were evaluated. Background Oleuropein is a flavonoid, which has potent anti‐inflammatory and bone‐protective effects. Methods Thirty‐two Wistar rats were divided into four experimental groups as following: control (C, n = 8) group; periodontitis (P, n = 8) group; periodontitis and low‐dose oleuropein group (12 mg/kg/day oleuropein, LDO group, n = 8); and periodontitis and high‐dose oleuropein group (24 mg/kg/day oleuropein, HDO group, n = 8). Periodontitis was induced via ligatures. Study period was 14 days, and animals were sacrificed at end of this period. Mandibles were examined via a stereomicroscope and underwent histological procedures. Osteoblast, tartrate‐resistant acid phosphatase (TRAP)‐positive osteoclast, and inflammatory cell counts were determined in hematoxylin‐eosin stained sections. Inducible nitric oxide synthase (iNOS), bone morphogenetic protein‐4, the cluster of differentiation (CD)‐68, cysteine‐aspartic proteases‐3 (Caspase 3), and B‐cell lymphoma‐2 (Bcl‐2) expressions were evaluated via immunohistochemistry. Results Periodontitis group had highest alveolar bone loss, and these levels significantly decreased in LDO and HDO groups. Both 12 and 24 mg/kg oleuropein groups significantly increased osteoblast cell counts and decreased TRAP‐positive osteoclast and inflammatory cell counts. BMP‐4 and bcl‐2 expressions were elevated in oleuropein groups while caspase‐3 expressions decreased. iNOS and CD68 were higher in periodontitis group compared to control group, but there was no significant difference between other groups. Conclusion Oleuropein successfully decreased alveolar bone loss as a result of decreased osteoclastic activity, inflammation, and apoptosis and increased osteoblastic activity.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Histologic Evaluationmentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of the effect of oleuropein on alveolar bone loss, inflammation, and apoptosis in experimental periodontitis

Taşkan

Yüce

Karataş

et al. 2019

J of Periodontal Research

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“…Fibroblast, endothelial, and inflammatory cells which can be easily differentiated was observed, and fibroblast and inflammatory cells within the frame was counted. Inflammatory cells counted were macrophage, neutrophil, eosinophil, T lymphocytes, and plasma cells . The cells which were not easily differentiated were not counted.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of apoptosis and hypoxia‐related factors in gingival tissues of smoker and non‐smoker periodontitis patients

Karataş

Yüce

Tülü

et al. 2019

J of Periodontal Research

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Objective: Smoking causes pathological changes in all tissues, including gingiva and alveolar bone. The aim of present study was to evaluate apoptotic tissue alterations and tissue destruction in smoker and non-smoker periodontitis patients and healthy individuals. Methods: Gingival biopsy samples from 15 systemically and orally healthy individuals (Group 1), 15 systemically healthy periodontitis patients (Group 2), 15 systemically and orally healthy smokers (Group 3), and 15 systemically healthy smoker periodontitis patients (Group 4) were enrolled in the present study. Clinical periodontal measurements as plaque index (PI), gingival index (GI), and clinical attachment levels (CAL) were recorded, and gingival biopsies were obtained. Biopsy samples were fixed in formalin solution and embedded in paraffin. Fibroblast and inflammatory cell counts were determined via histomorphometrically. Hypoxia-inducible factor alpha (HIF-1α), vascular endothelial growth factor(VEGF), tissue inhibitor of matrix metalloproteinase-1(TIMP-1), matrix metalloproteinases-8(MMP-8) expressions, Bax, Bcl-2, and caspase-3 expressions were evaluated via immunohistochemistry.Results: Demographic data of the study groups were similar. Smoking levels of the smokers were also similar. The highest fibroblast cell counts were observed in healthy controls and the counts were similar in other groups. The highest inflammatory cell counts were found in smoker periodontitis group, and the lowest counts were found in healthy control groups. The differences were statistically significant. HIF-1α and Bax expressions were elevated and Bcl-2 decreased in smoker periodontitis patients compared with healthy individuals. However, there were no differences in VEGF, MMP-8, and TIMP-1 expressions. Conclusion:Within limits of present study, it can be suggested that both smoking and periodontitis caused similar decrease in fibroblast counts while causing a dramatic increase in inflammatory cell counts. Increased apoptosis and hypoxia also accompanied to the increased inflammation. K E Y W O R D S apoptosis, hypoxia, smoking | 393 KARATAS eT Al.

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Effects of the Association of Periodontitis and Type 1 Diabetes Mellitus Induced on Periodontal Tissues and the Duodenal Mucosa of Wistar Rats

et al. 2020

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The effect of commercial conjugated linoleic acid products on experimental periodontitis and diabetes mellitus in Wistar rats

Abstract: Within the limits of this study, commercial CLA product administration in addition to diet significantly reduced alveolar bone loss, increased osteoblastic activity and decreased osteoclastic activity in the diabetic Wistar rats.

Cited by 20 publications

References 67 publications

Evaluation of the effect of oleuropein on alveolar bone loss, inflammation, and apoptosis in experimental periodontitis

Evaluation of the effect of oleuropein on alveolar bone loss, inflammation, and apoptosis in experimental periodontitis

Evaluation of apoptosis and hypoxia‐related factors in gingival tissues of smoker and non‐smoker periodontitis patients

Effects of the Association of Periodontitis and Type 1 Diabetes Mellitus Induced on Periodontal Tissues and the Duodenal Mucosa of Wistar Rats

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