The effect of copper nanoparticles and copper (II) salt on redox reactions and epigenetic changes in a rat model

Ognik, Katarzyna; Cholewińska, Ewelina; Juśkiewicz, Jerzy; Zduńczyk, Zenon; Tutaj, Krzysztof; Szlązak, Radosław

doi:10.1111/jpn.13025

Cited by 29 publications

(34 citation statements)

References 61 publications

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“…8-OHdG is adjacent to 5-methylcytosine. Frequent conversion of 5-methylcytosine to 5-hydroxymethylcytosine also changes binding affinity for MBP, which leads to epigenetic modifications [35,36]. This study demonstrated that the high Met level induced DNA methylation in the wall of the ileum, which was not observed in turkeys fed diets with low Met content.…”

Section: Itemmentioning

confidence: 68%

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The effect of different dietary ratios of lysine and arginine in diets with high or low methionine levels on oxidative and epigenetic DNA damage, the gene expression of tight junction proteins and selected metabolic parameters in Clostridium perfringens-challenged turkeys

Ognik

Konieczka

Mikulski

et al. 2020

Vet Res

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Two experiments were performed to investigate the effect of different ratios of arginine (Arg) to lysine (Lys) in diets with low (30% Lys; Experiment 1) and high (45% Lys; Experiment 2) methionine (Met) levels on selected metabolic parameters, oxidative and epigenetic DNA damage, and the mechanisms underlying intestinal barrier integrity in turkeys challenged with Clostridium perfringens. In each experiment, 108 one-day-old Hybrid Converter female turkeys were placed in 6 pens (18 birds per pen) and reared for 42 days. At 34, 36 and 37 days of age, half of the birds were subjected to C. perfringens challenge. A 3 × 2 factorial design with three levels of Arg relative to Lys (90, 100 and 110%; Arg 90 , Arg 100 and Arg 110 , respectively) and C. perfringens infection (−, +) was employed. Challenging birds with C. perfringens increased lipid oxidation and the oxidation and methylation of DNA of intestinal mucosa, and down-regulated the activities of DNA-repairing enzymes. Neither the dietary treatment nor the challenge affected the markers of liver function or metabolism. Arg 110 diets with the high Met level induced DNA oxidation and methylation whereas these processes were downregulated in birds fed Arg 90 diets. The results indicate that Arg 90 diets with high Met levels have a beneficial influence on the indicators of intestinal barrier integrity in turkeys with necrotic enteritis (NE). Despite the analyzed amino acid ratios interacted with the systems responsible for the maintenance of gut integrity in the host organism, this dietary intervention probably enabled birds to cope with NE.

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Section: Itemmentioning

confidence: 68%

“…The Lys, Met and Arg content of experimental diets was determined analytically again ( Table 1). The diets were offered as crumbles (days 1-28) and pellets (days [29][30][31][32][33][34][35][36][37][38][39][40][41][42].…”

Section: Experimental Design and Dietsmentioning

confidence: 99%

The effect of different dietary ratios of lysine and arginine in diets with high or low methionine levels on oxidative and epigenetic DNA damage, the gene expression of tight junction proteins and selected metabolic parameters in Clostridium perfringens-challenged turkeys

Ognik

Konieczka

Mikulski

et al. 2020

Vet Res

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“…The nano Cu particles (40-60 nm size nanopowder, 12 m 2 /g) were purchased from Sky Spring Nanomaterials, Inc. (Houston, TX, US), with a purity of 99.9% on a trace metals basis, with a spherical morphology of 0.19 g/cm 3 bulk density, and an 8.9 g/cm 3 true density. The zeta potential of NPs was determined to be −30.3 mV (phosphate-buffered saline), for more details see Ognik et al [12].…”

Section: Drugs and Reagentsmentioning

confidence: 99%

“…Modified response to ACh [6] and increased contraction to prostaglandin F 2 -alpha were observed [7] with no attenuation of endothelin-1-induced contraction [7]. Surprisingly, several studies have reported a beneficial anti-diabetic and cardioprotective role of CuNPs with a decreased production of inflammatory mediators [8,9,10,11,12].…”

Section: Introductionmentioning

confidence: 97%

Dietary supplementation with copper nanoparticles influences the markers of oxidative stress and modulates vasodilation of thoracic arteries in young Wistar rats

et al. 2020

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We aimed to study the physiological effects of diet supplemented with copper (Cu) nanoparticles (NPs). During the eight weeks of the experiment, young Wistar rats (at seven weeks of age, n = 9) were supplemented with 6.5 mg of Cu either as NPs or carbonate salt (Cu 6.5). A diet that was not supplemented with Cu served as a negative control (Cu 0). The impact of nano Cu supplementation on lipid (reflected as thiobarbituric acid reactive substances-TBARS) and protein peroxidation (thiol and carbonyl groups) in blood plasma as well as the influence on the vasodilatory mechanism(s) of isolated rat thoracic arteries were studied. Supplementation with Cu enhanced lipid peroxidation (TBARS) in NP 6.5 (x2.4) and in Cu 6.5 (x1.9) compared to the negative control. Significant increase in TBARS was also observed in NP 6.5 (x1.3) compared to the Cu 6.5 group. The level of thiol groups increased in NP 6.5 (x1.6) compared to Cu 6.5. Meanwhile, significant (x0.6) decrease was observed in the Cu 6.5 group compared to the negative control. Another marker of protein oxidation, carbonyl groups increased in NP 6.5 (x1.4) and Cu 6.5 (x2.3) compared to the negative control. However significant difference (x0.6) was observed between NP 6.5 and Cu 6.5. Arteries from Cu supplemented rats exhibited an enhanced vasodilation to gasotransmitters: nitric oxide (NO) and carbon monoxide (CO). An enhanced vasodilation to NO was reflected in the increased response to acetylcholine (ACh) and calcium ionophore A23187. The observed responses to ACh and CO releasing molecule (CORM-2) were more pronounced in NP 6.5. The activator of cGMP-dependent protein kinases (8-bromo-cGMP) induced similar vasodilation of thoracic arteries in NP 6.5 and Cu 0 groups, while an increased response was observed in the Cu 6.5 group. Preincubation with the inducible nitric oxide (iNOS) synthase inhibitor-1400W, decreased the ACh-induced vasodilation in NP 6.5 , exclusively. Meanwhile the eicosanoid metabolite of arachidonic acid (20-HETE) synthesis inhibitor-HET0016, enhanced vasodilation of arteries from Cu 0 group. In conclusion, this study demonstrates that supplementation with nano Cu influences oxidative stress, which further has modified the vascular response.

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“…A further recent study analyzed the effects of 4 week diet supplementation with Cu NPs in rats. The exposure not only caused reduced protein oxidation and nitration, but it also affected DNA oxidation and methylation [13].…”

Section: Introductionmentioning

confidence: 99%

DNA Methylation Profiles in a Group of Workers Occupationally Exposed to Nanoparticles

Rössnerová

Hoňková

Pelclová

et al. 2020

IJMS

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The risk of exposure to nanoparticles (NPs) has rapidly increased during the last decade due to the vast use of nanomaterials (NMs) in many areas of human life. Despite this fact, human biomonitoring studies focused on the effect of NP exposure on DNA alterations are still rare. Furthermore, there are virtually no epigenetic data available. In this study, we investigated global and gene-specific DNA methylation profiles in a group of 20 long-term (mean 14.5 years) exposed, nanocomposite, research workers and in 20 controls. Both groups were sampled twice/day (pre-shift and post-shift) in September 2018. We applied Infinium Methylation Assay, using the Infinium MethylationEPIC BeadChips with more than 850,000 CpG loci, for identification of the DNA methylation pattern in the studied groups. Aerosol exposure monitoring, including two nanosized fractions, was also performed as proof of acute NP exposure. The obtained array data showed significant differences in methylation between the exposed and control groups related to long-term exposure, specifically 341 CpG loci were hypomethylated and 364 hypermethylated. The most significant CpG differences were mainly detected in genes involved in lipid metabolism, the immune system, lung functions, signaling pathways, cancer development and xenobiotic detoxification. In contrast, short-term acute NP exposure was not accompanied by DNA methylation changes. In summary, long-term (years) exposure to NP is associated with DNA epigenetic alterations.

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The effect of copper nanoparticles and copper (II) salt on redox reactions and epigenetic changes in a rat model

Cited by 29 publications

References 61 publications

The effect of different dietary ratios of lysine and arginine in diets with high or low methionine levels on oxidative and epigenetic DNA damage, the gene expression of tight junction proteins and selected metabolic parameters in Clostridium perfringens-challenged turkeys

The effect of different dietary ratios of lysine and arginine in diets with high or low methionine levels on oxidative and epigenetic DNA damage, the gene expression of tight junction proteins and selected metabolic parameters in Clostridium perfringens-challenged turkeys

Dietary supplementation with copper nanoparticles influences the markers of oxidative stress and modulates vasodilation of thoracic arteries in young Wistar rats

DNA Methylation Profiles in a Group of Workers Occupationally Exposed to Nanoparticles

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