The effect of dietary supplementation of low crude protein on intestinal morphology in pigs

Li, Yunyun; Xiao, Lu; Wu, Haiqin; Mao, Xia; Hou, Qihang; Hu, Weiwei; Li, Tiejun; Wu, Li; Yu, Qinghua

doi:10.1016/j.rvsc.2018.11.013

Cited by 14 publications

(8 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, the numbers of goblet cells in the crypt of the cecum in the XOS500 treatment group was remarkably higher than the CON group. Goblet cells produce mucin glycoproteins, constituents of the mucus, which is in the first line of defense and protects the epithelium lining the intestinal mucosa from damage and invading pathogens (Strobel et al, 2015;Li et al, 2019). The increase of goblet cells numbers thus indicates that XOS500 supplementation contributes to an improvement of the chemical barrier (mucus layer) in the cecum.…”

Section: Discussionmentioning

confidence: 99%

Effects of Xylo-Oligosaccharides on Growth and Gut Microbiota as Potential Replacements for Antibiotic in Weaning Piglets

et al. 2021

View full text Add to dashboard Cite

Xylo-oligosaccharides (XOS) is a well-known kind of oligosaccharide and extensively applied as a prebiotic. The objective of this study was to investigate the effect of XOS supplementation substituting chlortetracycline (CTC) on growth, gut morphology, gut microbiota, and hindgut short chain fatty acid (SCFA) contents of weaning piglets. A total of 180 weaned piglets were randomly allocated to three treatments for 28 days, as follows: control group (basal diet, CON), basal diet with 500 mg/kg (XOS500) XOS, and positive control (basal diet with 100 mg/kg CTC). Compared with the CON group, the piglets in the XOS500 group improved body weight (BW) on days 28, average daily gain (ADG) and reduced feed: gain ratio during days 1–28 (P < 0.05). The XOS500 supplementation increased Villus height and Villus height: Crypt depth ratio in the ileum (P < 0.05). Villus Height: Crypt Depth of the ileum was also increased in the CTC treatment group (P < 0.05). Meanwhile, the XOS500 supplementation increased significantly the numbers of goblet cells in the crypt of the cecum. High-throughput 16S rRNA gene sequencing revealed distinct differences in microbial compositions between the ileum and cecum. XOS500 supplementation significantly increased the bacterial diversity. However, CTC treatment markedly reduced the microbial diversity (P < 0.05). Meanwhile, XOS500 supplementation in the diet significantly increased the abundance of Lactobacillus genus compared to the CON and CTC group in the ileum and cecum (P < 0.01), whereas the level of Clostridium_sensu_stricto_1, Escherichia-Shigella, and Terrisporobacter genus in the XOS500 group were markedly lower than the CON and CTC group (P < 0.05). In addition, dietary supplementation with XOS500 significantly increased the total short-chain fatty acids, propionate and butyrate concentrations and decreased the acetate concentration compared to the CON group in the cecum (P < 0.05). In summary, dietary supplemented with XOS500 could enhance specific beneficial microbiota abundance and decrease harmful microbiota abundance to maintain the structure of the intestinal morphology and improve growth performance of weaned piglets. Thus, XOS may potentially function as an alternative to in-feed antibiotics in weaned piglets in modern husbandry.

show abstract

Section: Discussionmentioning

confidence: 99%

Effects of Xylo-Oligosaccharides on Growth and Gut Microbiota as Potential Replacements for Antibiotic in Weaning Piglets

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Goblet cells are an important secretory cell, which plays an important role in antigen presentation, immune response and mucus secretion (Kulkarni et al, 2018;Saban et al, 2019). Recently, a study in pigs showed that modest reduction of crude protein levels did not affect intestinal morphology (number of goblet cells) and mucin-2 and tight junction proteins (Li et al, 2019); however, the number of goblet cells was significantly reduced in the low-protein group, the reason might be associated with dietary protein level as 6% reduction in protein level were used in this study.…”

Section: Discussionmentioning

confidence: 99%

Effects of dietary protein on gut development, microbial compositions and mucin expressions in mice

Tang

Gong

et al. 2022

Journal of Applied Microbiology

View full text Add to dashboard Cite

Aims: Dietary protein, as an important macronutrient, widely participates in host growth and metabolism. In this study, effects of different protein levels (14, 20 and 26%) on the gut development, microbial compositions and mucin expressions were studied in C57BL/6 mice. Methods and Results:The results showed that body weight and the relative weight of stomach and gut were decreased in low-protein diet-fed mice, whereas highprotein diet significantly reduced the villus length and area of jejunum. Goblet cells number in the jejunum was reduced in the low-protein group, which was reversed by dietary a high-protein diet. In addition, high-protein diet notably reduced microbial diversity and changed the microbial compositions at the phylum level, such as Bacteroides, Proteobacteria, Actinomycetes and Deferribacteres. Furthermore, highprotein diet significantly increased mucin2, mucin3 and mucin4 expressions in the jejunum, but downregulated mucin1, mucin2, mucin4 and TFF3 in the ileum, indicating a tissue-dependent manner.Conclusions: Together, high-protein diet may impair gut development, microbial balance and mucin system, and a low-protein diet is suggested to promote a healthy lifestyle.Significance and Impact of Study: Mucin influenced gut development (villus index and goblet cell number) through remodelling gut microbes, as low and high protein levels resulted in contrary expression levels of mucin in jejunum and ileum.

show abstract

“…Modifications of intestinal morphology, mainly increasing VH and the VH/CD in the duodenum and jejunum, could improve nutrient absorption ( Schiavone et al, 2018 ; Hajimohammadi et al, 2020 ). Small intestinal VH determined the functional maturity of villus absorptive cells while the VH increases; the intestinal surface area enabled more efficient absorption of available nutrients ( Li et al, 2019 ; Ramlucken et al, 2019 ). In this study, the supplementation of 1% HILM significantly increased the VH of the duodenum, and dietary 1 and 3% HILM supplementation had no damage effect in the duodenum and jejunum.…”

Section: Discussionmentioning

confidence: 99%

Black Soldier Fly (Hermetia illucens) Larvae Meal Modulates Intestinal Morphology and Microbiota in Xuefeng Black-Bone Chickens

Lei

Yao

et al. 2021

Front. Microbiol.

View full text Add to dashboard Cite

The addition of Hermetia illucens larvae meal (HILM) to the feed could contribute to particular antimicrobial and intestinal health in animal husbandry. This study was conducted to investigate the effects of HILM on intestinal morphology and microbial diversity in different intestinal segments of Xuefeng black-bone chickens. All of 432 birds (45 weeks old) were randomly assigned to four equal groups with six replicates and 18 hens in each replicate: (A) basal diet, (B) basal diet with 1% HILM, (C) basal diet with 3% HILM, and (D) basal diet with 5% HILM. The results showed that, compared with the basal diet group, the HILM supplement significantly increased the abundance-based coverage estimator (ACE) and Chao index in cecum (p < 0.05). Diet with 1% HILM significantly increased the villus height (VH) of the duodenum (p < 0.05) and cecum microbial diversity as represented by the Simpson index (p < 0.05). In particular, 1% HILM displayed a markedly increase in the genus unclassified Bacteroidales (cecum, p < 0.05). A basal diet with 3% HILM markedly increased the beneficial genus Romboutsia (jejunum, p < 0.05). Also, principal component analysis (PCA) cluster analysis showed that 3% of HILM was more individual than other groups (p < 0.05). However, 5% HILM decreased the VH and the ratio of villus height to crypt depth (VH/CD) of the jejunum and increased beneficial bacteria such as Staphylococcus (p < 0.05), which was regarded as pathogenetic genera. In conclusion, we found that HILM improved intestinal morphology and increased microbiological diversity and species abundance. Together, dietary supplementation of 1 or 3% HILM might benefit the intestinal morphology and intestinal microbiota of Xuefeng black-bone chicken. However, the addition of 5% HILM could decrease VH and the ratio of VH/CD of the jejunum and increased pathogenetic genera. HILM was an excellent protein substitute for Xuefeng black-bone chickens, which could meet the nutritional requirements under the condition of less feed. These results provide information for HILM meal as an alternative source of soybean meal in Xuefeng black-bone chickens’ feed.

show abstract

The effect of dietary supplementation of low crude protein on intestinal morphology in pigs

Cited by 14 publications

References 31 publications

Effects of Xylo-Oligosaccharides on Growth and Gut Microbiota as Potential Replacements for Antibiotic in Weaning Piglets

Effects of Xylo-Oligosaccharides on Growth and Gut Microbiota as Potential Replacements for Antibiotic in Weaning Piglets

Effects of dietary protein on gut development, microbial compositions and mucin expressions in mice

Black Soldier Fly (Hermetia illucens) Larvae Meal Modulates Intestinal Morphology and Microbiota in Xuefeng Black-Bone Chickens

Contact Info

Product

Resources

About