The effect of different acute concentrations of cadmium chloride on the frequency of micronuclei in AO rats

Abstract

Cadmium (Cd) is highly toxic heavy metal which may cause severe biological effects in vivo and in vitro. In this study, an evaluation of the acute Cd ability to trigger micronuclei (MNi) formation was carried out on 3-monthold male and female Albino Oxford (AO) rats using micronucleus (MN) test. Experimental animals were treated intraperitoneally with three different concentrations of cadmium chloride (CdCl2): 0.5, 1, and 2 mg CdCl2 per kg of body weight. Control animals received equal volume of sterile phosphate buffered saline. The results showed that 2 mg CdCl2 per kg b.w. concentration caused a highly statistically significant (P < 0.001) increase in MNi formation in the bone marrow polychromatic erythrocytes (PCEs), exerting a clear-cut concentration-dependent effect. Lower concentrations of CdCl2 used (0.5 and 1 mg/kg b.w.) also caused MNi formation, but with lower statistical significance. Sex differences in MNi production in bone marrow PCEs after acute exposure to different experimental concentrations of CdCl2 were not observed in our study. Our results indicate the ability of CdCl2 to exerts genotoxic effects in bone marrow of AO rats, and complement previous data on the genotoxicity of this important environmental contaminant, burdening the body from different sources - major being industrial exposure, food and cigarette smoking. [Projekat Ministarstva nauke Republike Srbije, br. III46002]

Induction of micronuclei by cadmium chloride in AO rats depends on age and sex

“…The bone marrow of laboratory rodents is routinely used for evaluation of clastogenic and/or aneugenic effects (ÇELIK et al, 2009;DJELIĆ et al, 2006;BAJIĆ et al, 2008;DJELIĆ et al, 2008). Based upon the intriguing results of our previous study (POPOVIĆ-BUBUJUK et al, 2013) that showed the ability of CdCl 2 to induce micronuclei formation in three months old AO rats, regardless of sex, this study broadened the research by including 6 and 12 months old AO rats.…”

The influence of chitosan oligosaccharide on some hematological parameters in rats exposed to cadmium

“…Furthermore, chronic Cd toxication leads to neoplastic-like alterations in the morphology of lymphocytes and monocytes, as well as hemolysis, vacuolization, denuclearization, and dislocation of the nucleus in erythrocytes (SULJEVIC et al, 2019). It was also suggested that chronic exposure to Cd showed cytotoxic and genotoxic effects on peripheral blood and bone marrow cells (POPOVIC-BUBUJUK et al, 2013).…”

Influence of cadmium chloride on the frequency of micronuclei in DA and AO rats

“…We previously suggested that Cd induces micronuclei in bone marrow cells. Our previous study showed the ability of Cd to induce micronuclei formation in three months old AO rats (POPOVIĆ-BUBUJUK et al, 2013). Later, we included 6 and 12 month old AO rats of both sexes.…”