The effect of different doses of methisoprinol on the percentage of CD4+ and CD8+ T lymphocyte subpopulation and the antibody titers in pigeons immunised against PPMV-1

Abstract

As immunosuppression in pigeons is common and results in reduced post-vaccination immunity and lower health status of the birds, studies have been taken up aimed at evaluation of the effect of three doses of methisoprinol on the percentage of CD4 + and CD8 + T lymphocyte subpopulation in peripheral blood and in the spleen and the titre of anti-NDV antibodies in the serum of pigeons in four groups (A, B, C, D), with 20 birds each. Pigeons in each group were immunised against paramyxovirosis at week 6 and 9 of life. Water for injection (group A -control) or methisoprinol at 100 mg/ kg of body weight (group B), 200 mg/kg of body weight (group C) and 600 mg/kg of body weight (group D) was administered intramuscularly for 3 days before each vaccination. The immunological analyses were carried out by flow cytometry and the ELISA test.The findings indicate that methisoprinol administered intramuscularly at 100 and 200 mg/kg of body weight for 3 successive days before vaccination against paramyxovirosis mainly stimulates the mechanisms of non-specific humoral and cellular immunity, which is indicated by a higher percentage of the subpopulation of CD4 + T lymphocytes in peripheral blood and in the spleen and a higher titre of anti-NDV antibodies.

The epidemiology, molecular characterization and clinical pathology of circovirus infections in pigeons – current knowledge

“…Attempts have also been made to immunize parrots with the recombinant capsid protein of the psittacine circovirus, but the efficacy of the vaccine was not confirmed (Bonne et al 2009). In view of the above, the only available solution for controlling the spread of PiCV infections involves the prevention of common viral and bacterial diseases in pigeons, the use of immunomodulators during vaccination and weaning, elimination of stress, and selection of parent stock based on the results of serological and molecular tests (Duchatel & Szeleszczuk 2011;Stenzel et al 2011;Stenzel et al 2016). …”

Impact of CpG oligodeoxynucleotide stimulation on percentage of T and B cells in chicken

“…The bursa and spleen cells were prepared as described previously (Chrząstek et al 2011, Stenzel et al 2011. For immunophenotypic analyses, aliquots of bursa lymphocytes (1x10 6 cells/ml) were incubated with FITC-conjugated Bu-1 monoclonal antibody (Clone AV 20, chB6) (SouthernBiotech, Birmingham, AL, USA), in PBS for 30 min, and then washed three times with PBS (pH 7.4).…”

“…Peripheral blood mononuclear cells (PBMC) to be used for the flow cytometric phenotypings were purified as described previously by Stenzel et al (2011). Aliquots of lymphocytes were then incubated with different mouse anti chicken monoclonal antibodies: R-PE-CD3 (clone CT-3), FITC-CD4 (clone CT-4), R-PE-CD8α (clone CT-8), FITC-Bu-1 (clone AV20), RPE-MHCII (clone 2G11), FITC-TCRγδ (clone TCR 1) obtained from Southern Biotech (Birmingham, AL, USA) for 30 min, and then washed three times with PBS (pH=7.4).…”

Acute and Recurrent Respiratory Tract Infections: Possible Reduction of the Patient’s Pill Burden

“…Стимулируя дифференциров-ку Т лимфоцитов в цитотоксические клетки и Т хелперы, а также повышая продукцию лимфокинов, препарат спо-собствует выработке интерлейкинов 1, 2, интерферо-на ␥. Инозин пранобекс повышает функциональную активность NK-клеток; усиливая хемотаксис макрофа-гов и фагоцитоз, он потенцирует морфофункциональ-ную состоятельность нейтрофилов и моноцитов [52,53]. В то же время, стимулируя дифференцировку В лимфоци-тов в плазматические клетки и повышая продукцию анти-тел, препарат нормализует гуморальный иммунитет.…”