The Effect of Different Pollination on the Expression of Dangshan Su Pear MicroRNA

Cheng, Xi; Yan, Chongchong; Zhang, Jinyun; Ma, Chenhui; Li, Shumei; Jin, Qing; Zhang, Nan; Cao, Yunpeng; Lin, Yi; Cai, Yongping

doi:10.1155/2017/2794040

Cited by 13 publications

(21 citation statements)

References 43 publications

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“…Genomic comparisons are a comparatively quick and active way to transfer genomic knowledge acquired in one taxon to another ( Lyons et al, 2008 ). In this study, synteny analysis was carried out using MCScanX software and whole genome sequences to visualize the locations of homologous or orthologous genes ( Cao et al, 2016 ; Cheng et al, 2017 ). The identification of orthologous GhZHD genes will further define the evolutionary history of this gene family.…”

Section: Resultsmentioning

confidence: 99%

Zinc Finger-Homeodomain Transcriptional Factors (ZHDs) in Upland Cotton (Gossypium hirsutum): Genome-Wide Identification and Expression Analysis in Fiber Development

Abdullah

Cheng

et al. 2018

Front. Genet.

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Zinc finger-homeodomain (ZHD) genes encode a family of plant-specific transcription factors that not only participate in the regulation of plant growth and development but also play an important role in the response to abiotic stress. The ZHD gene family has been studied in several model plants, including Solanum lycopersicum, Zea mays, Oryza sativa, and Arabidopsis thaliana. However, a comprehensive study of the genes of the ZHD family and their roles in fiber development and pigmentation in upland cotton has not been completed. To address this gap, we selected a brown fiber cultivar for our study; brown color in cotton is one of the most desired colors in the textile industry. The natural colored fibers require less processing and little dying, thereby eliminating dye costs and chemical residues. Using bioinformatics approaches, we identified 37 GhZHD genes from Gossypium hirsutum and then divided these genes into seven groups based on their phylogeny. The GhZHD genes were mostly conserved in each subfamily with minor variations in motif distribution and gene structure. These genes were largely distributed on 19 of the 26 upland cotton chromosomes. Among the Gossypium genomes, the paralogs and orthologs of the GhZHD genes were identified and further characterized. Furthermore, among the paralogs, we observed that the ZHD family duplications in Gossypium genomes (G. hirsutum, G. arboreum, and G. raimondii) were probably derived from segmental duplication or genome-wide duplication (GWD) events. Through a combination of qRT-PCR and proanthocyanidins (PA) accumulation analyses in brown cotton fibers, we concluded that the candidate genes involved in early fiber development and fiber pigment synthesis include the following: GhZHD29, GhZHD35, GhZHD30, GhZHD31, GhZHD11, GhZHD27, GhZHD18, GhZHD15, GhZHD16, GhZHD22, GhZHD6, GhZHD33, GhZHD13, GhZHD5, and GhZHD23. This study delivers insights into the evolution of the GhZHD genes in brown cotton, serves as a valuable resource for further studies, and identifies the conditions necessary for improving the quality of brown cotton fiber.

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Section: Resultsmentioning

confidence: 99%

Zinc Finger-Homeodomain Transcriptional Factors (ZHDs) in Upland Cotton (Gossypium hirsutum): Genome-Wide Identification and Expression Analysis in Fiber Development

Abdullah

Cheng

et al. 2018

Front. Genet.

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“…In addition, a PPR protein (pyrus_GLEAN_10024043) was involved in plant development and RNA metabolism [ 56 ]. Given that miRNA could affect lignin metabolism and stone cell development via the regulation of laccases, this result suggests that the PPR protein may interact with RNA to control stone cell development [ 2 , 57 ]. PP2C25 (CUFF51.591.1) and NUDTs (pyrus_GLEAN_10031740 and pyrus_GLEAN_10035206) play roles in phosphorus modification and the hydrolysis of phosphorus-containing substances, respectively [ 58 , 59 ].…”

Section: Discussionmentioning

confidence: 99%

“…This feature is a key factor in the high stone cell content of the CD fruit. PbSAD expression in CD fruit was significantly reduced compared with CL fruit, suggesting that the G/S ratio of CD fruit was increased compared with CL fruit, resulting in lignin stability and difficulties in degrading and forming highly aggregated SCCs [ 2 , 13 ]. 2.…”

Section: Discussionmentioning

confidence: 99%

“…Dangshan Su is a pear variety originating in China that is known for its fruit, which bears a wealth of nutritional and medicinal value. ‘Dangshan Su’ has relatively high stone cell content in the fruit, which is a crucial factor influencing the quality of pear fruit by affecting not only sucrose and other nutrient contents but also flesh hardness and chewiness [ 1 , 2 ]. The ‘Lianglizaosu’ variety originated from a natural bud sport of ‘Dangshan Su’ pear.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of the transcriptomic analysis between two Chinese white pear (Pyrus bretschneideri Rehd.) genotypes of different stone cells contents

et al. 2017

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Stone cell content is thought to be one of the key determinants for fruit quality in pears. However, the molecular mechanism of stone cell development remains poorly understood. In this study, we found that the stone cell clusters (SCCs) distribution and area in 'Dangshan Su' (with abundant stone cells) were higher as compared to 'Lianglizaosu' (low stone cell content bud sport of 'Dangshan Su') based on the histochemical staining, and the correlations of lignin content with stone cell content and SCC area was significant. The fruits of 'Dangshan Su' and 'Lianglizaosu' at three different developmental stages (23 and 55 days after flowering and mature) were sampled for comparative transcriptome analysis to explore the metabolic pathways associated with stone cell development. A total of 42444 unigenes were obtained from two varieties, among which 7203 differentially expressed genes (DEGs) were identified by comparison of the six transcriptomes. Specifically, many DEGs associated with lignin biosynthesis were identified, including coumaroylquinate 3-monooxygenase (C3H), shikimate O-hydroxycinnamoyltransferase (HCT), ferulate 5-hydroxylase (F5H), cinnamyl alcohol dehydrogenase (CAD) and peroxidase (POD), as well as genes related to carbon metabolism, such as sorbitol dehydrogenase-like (SDH-like) and ATP-dependent 6-phosphofructokinase (ATP-PFK). At the peak of the stone cell content (55 days after flowering), the expression level of these genes in 'Dangshan Su' was significantly increased compared with 'Lianglizaosu', indicating that these genes were closely related to stone cell development. We validated the transcriptional levels of 33 DEGs using quantitative realtime polymerase chain reaction (qRT-PCR) analysis. The results were consistent with the transcriptome analysis, indicating the reliability of transcriptome data. In addition, subcellular localization analysis of three DEGs in lignin synthesis (PbC3H, PbF5H and PbPOD) revealed that these proteins are mainly distributed in the cell membrane and cytoplasm. These results provide new insights into the molecular mechanism of stone cell formation.

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“…Recent studies have demonstrated that the content and size of stone cells are not only determined by the genotype of pear varieties, but also by the external environment and cultivation methods, such as pollination, water, bagging, and exogenous mineral elements [7,[21][22][23][24]. Pears are gametophytic self-incompatible plants and they cannot be inseminated by self-pollination [2].…”

Section: Introductionmentioning

confidence: 99%

Effects of Metaxenia on Stone Cell Formation in Pear (Pyrus bretschneideri) Based on Transcriptomic Analysis and Functional Characterization of the Lignin-Related Gene PbC4H2

Cheng

Zhang

Wang

et al. 2020

Forests

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The deposition of lignin in flesh parenchyma cells for pear stone cells, and excessive stone cells reduce the taste and quality of the fruit. The effect of metaxenia on the quality of fruit has been heavily studied, but the effect of metaxenia on stone cell formation has not been fully elucidated to date. This study used P. bretschneideri (Chinese white pear) cv. ‘Yali’ (high-stone cell content) and P. pyrifolia (Sand pear) cv. ‘Cuiguan’ (low-stone cell content) as pollination trees to pollinate P. bretschneideri cv. ‘Lianglizaosu’ separately to fill this gap in the literature. The results of quantitative determination, histochemical staining and electron microscopy indicated that the content of stone cells and lignin in YL fruit (‘Yali’ (pollen parent) × ‘Lianglizaosu’ (seed parent)) was significantly higher than that in CL fruit (‘Cuiguan’ (pollen parent) × ‘Lianglizaosu’ (seed parent)). The transcriptome sequencing results that were obtained from the three developmental stages of the two types of hybrid fruits indicated that a large number of differentially expressed genes (DEGs) related to auxin signal transduction (AUX/IAAs and ARFs), lignin biosynthesis, and lignin metabolism regulation (MYBs, LIMs, and KNOXs) between the CL and YL fruits at the early stage of fruit development. Therefore, metaxenia might change the signal transduction process of auxin in pear fruit, thereby regulating the expression of transcription factors (TFs) related to lignin metabolism, and ultimately affecting lignin deposition and stone cell development. In addition, we performed functional verification of a differentially expressed gene, PbC4H2 (cinnamate 4-hydroxylase). Heterologous expression of PbC4H2 in the c4h mutant not only restored its collapsed cell wall, but also significantly increased the lignin content in the inflorescence stem. The results of our research help to elucidate the metaxenia-mediated regulation of pear stone cell development and clarify the function of PbC4H2 in cell wall development and lignin synthesis, which establishes a foundation for subsequent molecular breeding.

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The Effect of Different Pollination on the Expression of Dangshan Su Pear MicroRNA

Cited by 13 publications

References 43 publications

Zinc Finger-Homeodomain Transcriptional Factors (ZHDs) in Upland Cotton (Gossypium hirsutum): Genome-Wide Identification and Expression Analysis in Fiber Development

Zinc Finger-Homeodomain Transcriptional Factors (ZHDs) in Upland Cotton (Gossypium hirsutum): Genome-Wide Identification and Expression Analysis in Fiber Development

Comparison of the transcriptomic analysis between two Chinese white pear (Pyrus bretschneideri Rehd.) genotypes of different stone cells contents

Effects of Metaxenia on Stone Cell Formation in Pear (Pyrus bretschneideri) Based on Transcriptomic Analysis and Functional Characterization of the Lignin-Related Gene PbC4H2

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