The effect of Ehrlich ascites tumour growth on lactate dehydrogenase activities in tissues and physiological fluids of tumour bearing mice

Bengtsson, Gun; Andersson, Gunnar

doi:10.1016/0020-711x(81)90136-1

Cited by 7 publications

(1 citation statement)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Comparable results were previously reported and explained (Lundholm et al, 1978(Lundholm et al, , 1979Bengtsson and Andersson, 1981;and Ternell et al, 1985). The significant decreases in the levels of hepatic total protein, RNA and DNA in response to IAA treatment are in accordance with the findings of Glazer and Weber (1971), who reported a significant reduction in the total protein, RNA and DNA synthesis of newborn rat brain cortex slices after the addition of IAA due to one site of action for IAA on DNA synthesis, which may involve some processes concerned with the formation of DNA precursors, such as the sulfhydryl-sensitive ribonucleotide reductase complex.…”

Section: Discussionsupporting

confidence: 90%

Antitumor Activities of Iodoacetate and Dimethylsulphoxide Against Solid Ehrlich Carcinoma Growth in Mice

Fahim

Esmat²,

Mady³

et al. 2003

Biol. Res.

View full text Add to dashboard Cite

Treatment of tumor-bearing mice with LD 12.5 values of iodoacetate; IAA (1.84 mg/100g b.w.) and/or dimethylsulphoxide; DMSO (350 mg/ 100g b.w.) significantly increased the cumulative mean survival time and percentage of survivors and reduced the mean tumor weight, compared to tumor-bearing controls, however, a more pronounced effect is recorded in the combined treatment. Also, an increase in the life span (ILS%) and tumor growth inhibition ratio (T/C%) are reported and amounted to 145.78 and 43.80%, 195.54 and 61.30% and 220.77 and 78.40% in IAA, DMSO and combined-treated groups, respectively. Results obtained from biochemical studies reveal that a single IAA treatment of tumor-bearing mice significantly increased the levels of plasma lactate dehydrogenase (LDH) activity, while it also significantly decreased the levels of plasma glucose and liver total protein, RNA and DNA, compared to normal controls. On the other hand, a single DMSO treatment significantly elevated the activities of blood antioxidant enzymes, i.e. glutathione peroxidase (GP x ) and glucose-6-phosphate dehydrogenase (G6PDH) and decreased the liver RNA and DNA levels. Combined treatment increased significantly the levels of plasma LDH and erythrocytes G6PDH activities, as well as liver glycogen, and in contrast it decreased the levels of liver total protein, RNA and DNA, compared to normal controls.

show abstract

Section: Discussionsupporting

confidence: 90%

Antitumor Activities of Iodoacetate and Dimethylsulphoxide Against Solid Ehrlich Carcinoma Growth in Mice

Fahim

Esmat²,

Mady³

et al. 2003

Biol. Res.

View full text Add to dashboard Cite

show abstract

A fluorescent redox dye. Influence of several substrates and electron carriers on the tetrazolium salt—formazan reaction of Ehrlich ascites tumour cells

Stellmach

Severin

1987

Histochem J

View full text Add to dashboard Cite

This study was performed to elaborate the best conditions for measuring the redox activity of Ehrlich ascites tumour cells by using a new tetrazolium salt, cyantolyl tetrazolium chloride (CTC). This tetrazolium salt forms a fluorescent water-insoluble formazan on reduction on the surface of intact vital cells. The influences of fixation and of various substrates and electron carriers on the cellular reduction of CTC were investigated quantitatively using an elution technique. The amount of formazan obtained after incubating vital cells with Meldola Blue as electron carrier was greater than that obtained with Methylene Blue, menadione, 2,6-dichloroindophenol, 1-methoxyphenazine methosulphate or phenazine methosulphate. Using flow cytometry, the formazan production per cell and, after staining the nuclear DNA, the distribution of the redox activity in the cell population can be visualized with satisfactory resolution. We conclude from our findings that dehydrogenases are only partially involved in the reduction of tetrazolium salts by intact cells and that a redox activity, probably related to a cell membrane-bound NAD(P)H-oxidase system, is mainly measured.

show abstract

Release of dopamine, noradrenaline and dopamine B-hydroxylase from mouse neuroblastoma

et al. 1984

View full text Add to dashboard Cite

The effect of Ehrlich ascites tumour growth on lactate dehydrogenase activities in tissues and physiological fluids of tumour bearing mice

Cited by 7 publications

References 29 publications

Antitumor Activities of Iodoacetate and Dimethylsulphoxide Against Solid Ehrlich Carcinoma Growth in Mice

Antitumor Activities of Iodoacetate and Dimethylsulphoxide Against Solid Ehrlich Carcinoma Growth in Mice

A fluorescent redox dye. Influence of several substrates and electron carriers on the tetrazolium salt—formazan reaction of Ehrlich ascites tumour cells

Release of dopamine, noradrenaline and dopamine B-hydroxylase from mouse neuroblastoma

Contact Info

Product

Resources

About