“…For application of the electric field to the cells, we used agar bridges; one end was placed in the container and the other end at the side of the cell chamber. Agar bridges permit the flow of current but block convection of toxic substances from the wires (Jorgensen, 1972), electrolysis and electro-osmosis (Steckel et al, 1984), as well as joule heating (Brighton et al, 1975). In the middle of the chamber there is a block of polycarbonate, which serves to insulate the two margins of the cover slip where the agar bridges are placed.…”