The objective of the study was to determine the combined and individual effects of adding Natural antioxidants (astaxanthin and magnolol) to the diet of laying hens (Lohman Brown) in the lipid Profile and some oxidation indicators in blood serum. 120 laying hens, aged 52 weeks, were used in the experiment, which ran from the 20th of March 2022 to the 15th of May 2022 (8 weeks). The hens were randomly allocated to eight experimental treatments, with five replicates for each treatment and three chickens for each replication, the treatments included: T1(Control), T2: vitamin E 300 mg kg-1 diet, T3 and T4: adding asta 200 and 400 mg kg-1 diet respectively, T5 and T6: adding mag 200 and 400 mg kg-1 diet respectively, and T7 and T8: Add a mixture of asta 100 mg kg-1 of diet + mag 100 mg kg-1 of diet and asta 200 mg kg-1 of diet + mag 200 mg kg-1 of diet respectively. The findings showed that supplementing the feed with natural antioxidants significantly reduced the levels of total cholesterol, LDL and HDL in the blood serum of laying hens compared with T1. Furthermore, compared to T1 there was an important increase in the blood serum activity of the enzymes glutathione peroxidase and catalase for all treatments that included natural antibiotics, when compared with the control treatment, treatments T6, T5, and T8 showed a significant decrease in the blood serum value of malondehyde (MDA). We conclude from the study that the health of aging Laying hens reared in Iraq during hot and moderate weather is improved by include naturally available antioxidants in their diet.