The Effect of Exogenous IAA and Kinetin on Nitrate Reductase, Nitrite Reductase and Glutamate Dehydrogenase Activities in Excised Pea Roots

Sahulka, J.

doi:10.1007/bf02933184

Cited by 24 publications

(4 citation statements)

References 15 publications

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“…There is the possibility that the high concentration of Cu reduced the mobile phase of Fe/Ca; thus, indirectly affected their contents in plant tissues (Uchimiya et al, 2011;Tiberg et al, 2016). IAA has shown to depress nitrate reductase activity in pea roots (Sahulka, 1972) and it could influence other reductases altering the general metabolisms of pea plants. On the other hand, 100 μM IAA plus either bCuO or nano-CuO increased leaf B by more than 150%, compared to IAA alone or no IAA (Table 2).…”

Section: Micro-and Macro-nutrient Concentrations In Green Pea Plant Tmentioning

confidence: 99%

Modulation of CuO nanoparticles toxicity to green pea (Pisum sativum Fabaceae) by the phytohormone indole-3-acetic acid

Ochoa

Medina-Velo

Barrios

et al. 2017

Science of The Total Environment

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The response of plants to copper oxide nanoparticles (nano-CuO) in presence of exogenous phytohormones is unknown. In this study, green pea (Pisum sativum) plants were cultivated to full maturity in soil amended with nano-CuO (10-100nm, 74.3% Cu), bulk-CuO (bCuO, 100-10,000nm, 79.7% Cu), and CuCl at 50 and 100mg/kg and indole-3-acetic acid (IAA) at 10 and 100μM. Results showed that IAA at 10 and 100μM, averaged over all Cu treatments, reduced the number of plants by ~23% and ~34%, respectively. IAA at 10μM, nano-CuO at 50mg/kg, b-CuO at 50mg/kg, and CuCl at 100mg/kg reduced pod biomass by about 50%. Although some combinations of IAA, mainly at 100μM, with the Cu compounds altered nutrient accumulation in tissues, none of them affected pod elements. Conversely, without IAA, nano-CuO at 50mg/kg, increased pod Fe and Ni by 258% and 325%, respectively, while bCuO at 100mg/kg increased pod Ni by 275%, compared with control. With IAA at 10μM, nano-CuO (100mg/kg) and bCuO (50mg/kg) increased stem Cu by ~84% and ~78%. When IAA increased to 100μM, nano-CuO and bCuO reduced stem Ca by 32% and 37%, and Mg by ~35%. Results suggest that both the nano-CuO and bCuO could improve the nutritional quality of pea pods, while exogenous IAA combined with Cu-based compounds could impact green pea production since these treatments reduced the number of plants and pod biomass.

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Section: Micro-and Macro-nutrient Concentrations In Green Pea Plant Tmentioning

confidence: 99%

Modulation of CuO nanoparticles toxicity to green pea (Pisum sativum Fabaceae) by the phytohormone indole-3-acetic acid

Ochoa

Medina-Velo

Barrios

et al. 2017

Science of The Total Environment

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“…It is known that auxin and cytokinin antagonistically act on meristem activity (Moubayidin et al 2009). A relationship between auxin and cytokinin and nitrogen metabolism has also been reported (Sahulka 1972;Mi et al 2008). As described above, sugars act as signaling compounds in the whole plant life cycle, and sugar sensing and sugar-induced signal transductions have been revealed to interact with phytohormones such as IAA, cytokinins, ABA, and gibberellins (GA) by comprehensive genetic analysis (Smeekens 2000, Gibson 2005.…”

Section: Introductionmentioning

confidence: 96%

Aluminum effect on starch, soluble sugar, and phytohormone in roots of Quercus serrata Thunb. seedlings

Moriyama

Tomioka

Kojima

et al. 2015

Trees

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Key message Glucose was a key substance as an energy source in the root growth promotion by Al, and ABA may relate to metabolism involved with its process. Abstract Generally, excess aluminum (Al) ions in soil solution are toxic to many cultivated plant species, but beneficial effects of Al for plant growth have been reported. Previously, we reported stimulation of root growth and nitrate reductase (NR) activity by Al. In this study, we focused on sugars, such as sucrose, glucose, and fructose, as energy sources and also signaling substances to regulate root growth. To understand the mechanism of root growth stimulation by Al, we investigated the change in concentration of sugars and phytohormones, and the activity of NR in roots using Quercus serrata seedlings. Ten-week-old Q. serrata seedlings were hydroponically cultured with nutrient solution containing 2.5 mM Al (pH 4.0) or 3.25 mM calcium (Ca) (pH 4.0) for 3 and 15 days. The growth of first lateral root and NR activity was stimulated for 3 and 15 days of Al treatment. The concentration of starch and sucrose decreased, while the concentration of glucose increased in the Al-treated roots. The concentration of abscisic acid (ABA) in Al-treated roots increased gradually throughout the experiment. From the present study, the mechanism of root growth promotion by Al involves a complex signaling network. We suggest that glucose is a key substance as an energy source and a signaling substance to promote root growth induced by Al and ABA may relate to nitrogen (N) and carbon (C) metabolism involved with the signaling network to promote root growth induced by Al.

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“…Sterilized seeds of pea (Pisum sativum L. cv. Jupiter) were germinated under aseptic conditions and further cultivated at 25 ~ in darkness (Sahulka 1972). For the preparation of enzyme extracts 7-9 days-old seedlings were used.…”

Section: Methodsmentioning

confidence: 99%

Proposed Enzymes of Auxin Biosynthesis and Their Regulation III. Some Properties of Pea Indolylacetaldehyde Oxidase

Kutáček¹,

Terziivanova-dimova²

1991

Biologia plant.

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Indole-3-acetaldehyde oxidase (IAAld-oxidase) occurs in pea in two forms, of which the first, more active enzyme, has its pH optimum at 4.5, while the second, barely half as active, has a pH optimum at 7.0. Only the pH 4.5 oxidase can be resolved from the acetone powder. Besides IAAId the more stable 1Aid was used as substrate in testing the enzymatic activity. The pea enzyme seems not to be a dismutase since indolylmethanol or indolylethanol were not formed as products. Pyridine nucleotide coenzymes did not activate the partially purified enzyme. The pH 4.5 oxidase was inhibited by more than 50 % by IAA > L-asp > tryptophol > indoleacetylaspartic acid > 2,4-D (at 1 mM concentration). The pH 7.0 oxidase was inhibited relatively more weakly, a stronger than 50 % inhibition was caused only by NAA > L-asp. The oxidases were clearly distinguished by the response to L-asparagine (1 mM): the activity of the pH 4.5 oxidase was increased (+ 12 %), while the activity of the pH 7.0 oxidase was decreased (-71%). In preliminary in vitro experiments the phytohormones (1 mM) kinetin and GA3 increased the conversion of IAAld to IAA, while ABA decreased it.

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The Effect of Exogenous IAA and Kinetin on Nitrate Reductase, Nitrite Reductase and Glutamate Dehydrogenase Activities in Excised Pea Roots

Cited by 24 publications

References 15 publications

Modulation of CuO nanoparticles toxicity to green pea (Pisum sativum Fabaceae) by the phytohormone indole-3-acetic acid

Modulation of CuO nanoparticles toxicity to green pea (Pisum sativum Fabaceae) by the phytohormone indole-3-acetic acid

Aluminum effect on starch, soluble sugar, and phytohormone in roots of Quercus serrata Thunb. seedlings

Proposed Enzymes of Auxin Biosynthesis and Their Regulation III. Some Properties of Pea Indolylacetaldehyde Oxidase

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