The Effect of Experimental Anaemia on the Levels of Glutathione and Glycolytic Enzymes of the Erythrocytes of Normal and Glutathione-deficient Merino Sheep

Abstract

The effects of experimental anaemia on the levels of reduced glutathione (GSH) and the activity of glycolytic enzymes in the erythrocytes of normal and GSH-deficient Merino sheep were investigated. There was a rise in red cell GSH levels in both groups of sheep; the magnitude of this response was, however, quite different. When expressed as a percentage of the initial value, the rise in GSH level was 18 % in normal and 263 % in GSH-deficient animals. There was also an increase in the activities of various enzymes following phlebotomy but this increase was similar in the two groups of sheep.

Erythrocyte Enzymes in Sheep: Comparison of Activity in Fetal, Newborn, Maternal and Nonpregnant Ewe Erythrocytes

“…Comparison of a number of red cell en zymes have been made in sheep with high and low intracellular potassium [4], Agar and his associates have also published studies of red cell enzymes in normal and glutathione deficient sheep [1,2,6] and in a number of breeds of sheep [5]. Maronpot [21] studied glucose-6-phosphate dehydrogenase in six breeds of Egyptian Fat-tailed sheep.…”

“…Although some studies of enzyme activi ties have been made in adult sheep erythro cytes [1][2][3][4][5][6]21], few data are currently avail able for fetal red cell enzyme activities [17], Measurements of activities throughout basal development provide important information and will permit investigations of experimen tally induced changes in metabolism during development.…”

Concentrations du glutathion érythrocytaire des bovins zébus et Ndama nigérians

“…At this time, erythrocyte GSH concentrations usually increase (4,12,20) due to the circulating Young erythrocytes which have higher GSH concentrations and greater GSH metabolizing enzyme activity than older erytrocytes (4,8,10,20).…”