The Effect of Exposure Process of Toluene To The Spermatogonia Cell a Rattus Strain Wistar

Abstract

Intoduction: Acute and chronic exposure to toluene at high doses is known to affect all organs of the body including the spermatogenesis process. In the industrial sector, the use of toluene as a solvent is still widely used, up to 10 million tons per year. The control over health problems that may occur is carried out by applying work exposure threshold values. This research aims to explore the effect of toluene exposure at the threshold value range on spermatogenesis.Method: This research used laboratory experiment on 30 male Wistar rats which were divided into five groups of different exposure levels, namely 12.5 parts per million (ppm], 25 ppm, 50 ppm, 100 ppm, and no exposure (control). Exposure was given for 4 hours daily over 14 days through a hood with measured release in the glass cage. The toluene exposure markers observed were Malondialdehyde (MDA) in the blood tissue and testicles using the Thiobarbituric Acid Reactive Substances (TBARS) method. The effect on the spermatogenicity process was assessed by counting the spermatogonia A cells of male Wistar rats with Periodic Acid Schiff (PAS) staining and is calculated by the Abercrombie formula. Analysis of the correlation between the level of exposure and its effect on the increase in malondialdehyde, and spermatogenesis was carried out using the Spearman correlation analysis.Result: There was a moderately positive correlation between levels of toluene exposure and plasma MDA levels (r = 0.42; p = 0.025). Meanwhile, on [the issue of] the quantity of spermatogonia cells, a high level of negative correlation with exposure levels was obtained (r = -0.68; p = 0.001).Conclusion: Toluene exposure in male Wistar rats within the range of threshold values influenced the increase in plasma MDA levels and decreased the Spermatogenia A cells. However, toluene exposure did not affect the testicular MDA levels of male Wistar rats.

Efektivitas Ekstrak Etanol Tauge (Vigna Radiata) Dalam Meningkatkan Kadar Glutation Peroksidase Pada Tikus Putih (Rattus Norvegicus) Yang Diinduksi Toluena

“…Hal ini menyebabkan tingginya pekerja yang bekerja di bidang industri. Industri yang menjamur di Indonesia, seperti pada industri cat, plastik, tinta, dan lem banyak yang menggunakan bahan kimia, salah satunya toluena yang digunakan sebagai pelarut organik (Iqbal et al. 2021;Prayogi et al, 2020).…”

“…Menurut Surat Edaran Menteri Ketenagakerjaan No.SE-01/MEN/1997, nilai ambang batas penggunaan toluena sebesar 50 ppm (Iqbal et al, 2021). Penggunaan toluena yang melebihi ambang batas atau dalam jangka waktu yang lama dapat menyebabkan penyakit akibat kerja (Cindy et al, 2022).…”