THE EFFECT OF EXTRACTION METHOD ON THE CHEMICAL COMPOSITION OF Lamium amplexicaule .L ETHANOL EXTRACT DETERMINED BY HPLC/MS-MS

Abstract

Lamium amplexicaule. L is a plant of the Lamiaceae family, widespread in Algeria and North Africa. In the literature, no detailed research elucidating the methods of extraction of phenolic compounds from this species is available. Therefore, the objective of this study was to evaluate the efficient method between cold and hot extraction conditions. Ethanolic extracts were obtained by maceration and Soxhlet apparatus. Phytochemical composition was determined by liquid chromatography mass spectrometry (LC-MS/MS) method. Results demonstrate that the major compound in both extracts was chlorogenic acid. However, 18 compounds were identified in the extract obtained by Soxhlet and 17 in that obtained by maceration. The quantification of these constituents showed higher amounts in the Soxhlet extract than the maceration one. Therefore, it can be concluded that the Soxhlet extraction is more efficient for L. amplexicaule than the maceration one.