The Effect of Hexanoyl Glycol Chitosan on the Proliferation of Human Mesenchymal Stem Cells

Jeong, Young-Hoon; Oh, Hye Min; Lee, Man Ryul; Kim, C‐Yoon; Joo, Chanyang; Park, Soon‐Jung; Song, Yun-Ho; Kang, Chang‐Hee; Chung, Hyung-Min; Kang, Sun-Woong; Huh, Kang Moo; Moon, Sung-Hwan

doi:10.3390/polym10080839

Cited by 9 publications

(3 citation statements)

References 41 publications

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“…In addition, the expression of genes associated with senescence was inhibited and mitochondrial functioning increased. Taken collectively, these results indicate that the capacity for sustained in vitro development of AD-MSCs is shown by the HGC [127]. The effects of nanostructures on the differentiation of stem cells are shown in figure 3.…”

Section: Other Cytotoxicity Studiesmentioning

confidence: 58%

Stem cells based in vitro models: trends and prospects in biomaterials cytotoxicity studies

Ahmed¹,

Ahmed²,

Masoud³

et al. 2021

Biomed. Mater.

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Advanced biomaterials are increasingly used for numerous medical applications from the delivery of cancer-targeted therapeutics to the treatment of cardiovascular diseases. The issues of foreign body reactions induced by biomaterials must be controlled for preventing treatment failure. Therefore, it is important to assess the biocompatibility and cytotoxicity of biomaterials on cell culture systems before proceeding to in vivo studies in animal models and subsequent clinical trials. Direct use of biomaterials on animals create technical challenges and ethical issues and therefore, the use of non-animal models such as stem cell cultures could be useful for determination of their safety. However, failure to recapitulate the complex in vivo microenvironment have largely restricted stem cell cultures for testing the cytotoxicity of biomaterials. Nevertheless, properties of stem cells such as their self-renewal and ability to differentiate into various cell lineages make them an ideal candidate for in vitro screening studies. Furthermore, the application of stem cells in biomaterials screening studies may overcome the challenges associated with the inability to develop a complex heterogeneous tissue using primary cells. Currently, embryonic stem cells, adult stem cells, and induced pluripotent stem cells are being used as in vitro preliminary biomaterials testing models with demonstrated advantages over mature primary cell or cell line based in vitro models. This review discusses the status and future directions of in vitro stem cell-based cultures and their derivatives such as spheroids and organoids for the screening of their safety before their application to animal models and human in translational research.

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Section: Other Cytotoxicity Studiesmentioning

confidence: 58%

Stem cells based in vitro models: trends and prospects in biomaterials cytotoxicity studies

Ahmed¹,

Ahmed²,

Masoud³

et al. 2021

Biomed. Mater.

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“…Preventing cells from adhering to the culture dish surface during spheroid formation is crucial [11][12][13]. HGC, a polysaccharide-based thermoreversible polymer and an ultralow-attachments biomaterial, has received much attention for its efficiency in spheroid formation and its biomedical applications [12][13][14][15][16]. In this study, we utilized HGC with optimized properties as an ULA coating biomaterial to reduce cell attachment to the culture dish surface.…”

Section: Synthesis and Characterization Of Hgc As An Ultralow-adhesio...mentioning

confidence: 99%

Robust and customizable spheroid culture system for regenerative medicine

Park,

Truong,

Park

et al. 2024

Biofabrication

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Three-dimensional cell spheroids show promise for the reconstruction of native tissues. Herein, we report a sophisticated, uniform, and highly reproducible spheroid culture system for tissue reconstruction. A mesh-integrated culture system was designed to precisely control the uniformity and reproducibility of spheroid formation. Furthermore, we synthesized hexanoyl glycol chitosan, a material with ultralow cell adhesion properties, to further improve spheroid formation efficiency and biological function. Our results demonstrate improved biological function in various types of cells and ability to generate spheroids with complex structures composed of multiple cell types. In conclusion, our spheroid culture system offers a highly effective and widely applicable approach to generating customized spheroids with desired structural and biological features for a variety of biomedical applications.

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“…The major advantage of chitosan is the existence of modifiable positions in its chemical structure. This gives chitosan the opportunity to be employed in different applications [3,4,5,6,7,8,9,10,11,12]. The modification of the chitosan molecule with (i) grafting (insert functional groups) or (ii) cross-linking reactions (unite the macromolecular chains with each other) leads to the formation of chitosan derivatives with superior properties, such as the enhancement of adsorption capacity and resistance in extreme media conditions, respectively.…”

Section: Introductionmentioning

confidence: 99%

Cost Estimation of Polymeric Adsorbents

et al. 2019

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One of the most promising techniques of recent research is adsorption. This technique attracts great attention in environmental technology, especially in the decontamination of water and wastewaters. A “hidden” point of the above is the cost of adsorbents. As can be easily observed in the literature, there is not any mention about the synthesis cost of adsorbents. What are the basic criteria with which an industry can select an adsorbent? What is the synthesis (recipe) cost? What is the energy demand to synthesize an efficient material? All of these are questions which have not been answered, until now. The reason for this is that the estimation of adsorbents’ cost is relatively difficult, because too many cost factors are involved (labor cost, raw materials cost, energy cost, tax cost, etc.). In this work, the first estimation cost of adsorbents is presented, taking into consideration all of the major factors which influence the final value. To be more comparable, the adsorbents used are from a list of polymeric materials which are already synthesized and tested in our laboratory. All of them are polymeric materials with chitosan as a substrate, which is efficiently used for the removal of heavy metal ions.

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The Effect of Hexanoyl Glycol Chitosan on the Proliferation of Human Mesenchymal Stem Cells

Cited by 9 publications

References 41 publications

Stem cells based in vitro models: trends and prospects in biomaterials cytotoxicity studies

Stem cells based in vitro models: trends and prospects in biomaterials cytotoxicity studies

Robust and customizable spheroid culture system for regenerative medicine

Cost Estimation of Polymeric Adsorbents

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