The effect of Lactobacillus casei extract on cervical cancer cell lines

Kim, Soo Nyung; Lee, Won Moo; Park, Kyoung Sik; Kim, Jong Bin; Han, Dae Jong; Bae, Jaeman

doi:10.5114/wo.2014.45292

Cited by 17 publications

(11 citation statements)

References 20 publications

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“…Strains of L. fermentum and L. plantarum inhibited apoptosis in HeLa epithelial cells and showed antagonistic activity against Candida albicans and G. vaginalis [ 46 ]. In another study, the viability of cells isolated from epidermoid carcinoma of the cervix (CaSki) and HeLa cells was maintained after incubation with different concentrations of Lactobacillus casei extract [ 47 ]. In contrast, cell death of HMVII cells increased significantly after incubation with the membrane-associated lipoproteins (LAMP) of U. parvum , U. urealyticum , M. hominis , and M. genitalium ; LAMP extracted from U. parvum and M. hominis induced 41% and 34% cell death, respectively, the highest values among the 4 species ( Figure 2 ).…”

Section: Resultsmentioning

confidence: 99%

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Potential of Maintaining a Healthy Vaginal Environment by TwoLactobacillusStrains Isolated from Cocoa Fermentation

Melgaço

Pessoa

Freire

et al. 2018

BioMed Research International

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Bacteria in the genera Mycoplasma and Ureaplasma do not have cell walls and therefore interact with host cells through lipid-associated membrane proteins (LAMP). These lipoproteins are important for both surface adhesion and modulation of host immune responses. Mycoplasma and Ureaplasma have been implicated in cases of bacterial vaginosis (BV), which can cause infertility, abortion, and premature delivery. In contrast, bacteria of the genus Lactobacillus, which are present in the vaginal microbiota of healthy women, are thought to inhibit local colonization by pathogenic microorganisms. The aim of the present study was to evaluate the in vitro interactions between lipoproteins of Mycoplasma and Ureaplasma species and vaginal lineage (HMVII) cells and to study the effect of Lactobacillus isolates from cocoa fermentation on these interactions. The tested Lactobacillus strains showed some important probiotic characteristics, with autoaggregation percentages of 28.55% and 31.82% for L. fermentum FA4 and L. plantarum PA3 strains, respectively, and percent adhesion values of 31.66 and 41.65%, respectively. The two strains were hydrophobic, with moderate to high hydrophobicity values, 65.33% and 71.12% for L. fermentum FA4 and L. plantarum PA3 in toluene. Both strains secreted acids into the culture medium with pH=4.32 and pH=4.33, respectively, and showed antibiotics susceptibility profiles similar to those of other lactobacilli. The strains were also able to inhibit the death of vaginal epithelial cells after incubation with U. parvum LAMP from 41.03% to 2.43% (L. fermentum FA4) and 0.43% (L. plantarum PA3) and also managed to significantly decrease the rate of cell death caused by the interaction with LAMP of M. hominis from 34.29% to 14.06% (L. fermentum FA4) and 14.61% (L. plantarum PA3), thus demonstrating their potential for maintaining a healthy vaginal environment.

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Section: Resultsmentioning

confidence: 99%

“…Mollicutes causing genital infections need to adhere and subsequently invade the cells of the genitourinary tract to obtain nutrients from the host cells [ 1 , 2 , 47 ]. During adhesion, human cells first interact with the plasma membrane of these microorganisms, specifically with their lipoproteins.…”

Section: Resultsmentioning

confidence: 99%

Potential of Maintaining a Healthy Vaginal Environment by TwoLactobacillusStrains Isolated from Cocoa Fermentation

Melgaço

Pessoa

Freire

et al. 2018

BioMed Research International

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“…The efficacy was attributed to inhibiting nuclear factor (NF)-κB and mTOR-mediated signaling (15). The direct treatment of a cervical cancer cell line with a cell-free extract of L. casei did not have any significant inhibitory effect on the proliferation of the cells [16]. This suggests that the effects observed with the L. casei lysate in the present study are because of an adjuvant-like activity in combination with the heated-4T1-cell lysate rather than its direct effects on tumor cells.…”

Section: Discussionmentioning

confidence: 99%

“…Among several strains, Lactobacillus casei possess immunomodulatory properties [13,14]. Recent studies have focused on the potential of L. casei as an adjuvant in cancer therapy [15,16].…”

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confidence: 99%

Immunotherapeutic efficacy of a Lactobacillus casei lysate as an adjuvant combined with a heated-4T1 mammary carcinoma cell lysate in a murine model of breast cancer

et al. 2016

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Background Immunotherapy, during which the immune system of the patient is manipulated to act against tumors has been among the most successful methods in the treatment of breast cancer, a leading cause of mortality among women worldwide. Objectives To investigate the immunotherapeutic efficacy of Lactobacillus casei lysate as an adjuvant in combination with a heated-4T1 mammary carcinoma cell lysate in a model of breast cancer. Methods After ethics committee approval of all animal procedures, a murine model of breast cancer was induced in BALB/c mice using 4T1 cells. These mice were immunized with a combination of lysates of heated 4T1 cells and L. casei. Subsequent changes in tumor size and weight, and the production of TNF-α, IL-2, IL-12, IL-17, and IL13 were measured. Lung weights were measured as an indicator of metastasis to other organs. Results The tumor size and weight in mice immunized with the combined vaccine were significantly reduced compared with controls. The combined immunotherapy altered the pattern of cytokine production to the advantage of antitumor immunity, and was significantly more potent than immunization with heated-4T1-cell lysate or L. casei lysate alone. Conclusions Coadministration of L. casei lysate enhanced the immunotherapeutic efficacy of the heated-4T1-cell lysate as a source of tumor-associated antigens. L. casei can potentially be used as an adjuvant combined with sources of tumor antigens in the treatment of cancers, and as a safe alternative to the current adjuvants that cause greater irritation to hosts. Further studies are required to clarify the mechanisms underlying these effects.

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“…Among Lactobacillus species, Lactobacillus casei is one of the most common probiotics with industrial applications. It has been reported to have positive effects on human health such as causing remarkable improvements in immunity [1,2], allergies [3] and cholesterol levels [3]. Over the past few years, scientists have proven that probiotics and their bio-compound can have many benefits.…”

Section: Introductionmentioning

confidence: 99%

A polyethylene glycol-based method for extraction of extracellular vesicles from Lactobacillus casei as vaccine delivery vehicle

Vargoorani

Modarressi

Motevaseli

et al. 2018

vacres

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Introduction The secretion of extracellular vesicles (EVs) has been neglected in Gram-positive bacteria due to the absence of an outer membrane and the difficulties of proper visualization. Here we aimed to prove that lactobacillus casei can secrete extracellular vesicles. Methods: EVs were extracted from Lactobacillus casei, cultured in De Man, Rogosa and Sharpe broth, using a polyethylene glycol (PEG) solution. The characteristics of the EVs were analyzed by electron microscopy, Dynamic Light Scattering (DLS) and SDS-PAGE. Results: The electron microscopy showed rounded vesicles with average diameter of 300 nm. The protein content of this nanostructure was 2.5 mg/ml with a protein pattern within the range of 10-200 kDa. DLS result showed populations of approximately 300 nm while the extracted EVs had a negative zeta potential. Conclusion: A new method of producing functional molecules from probiotic bacteria was presented. Our results indicated EVs purity with acceptable conformation. Further investigations are necessary to elucidate the efficacy, practicality and mechanism of action of such EVs in clinical practices, especially for development of bio-compounds and vaccine delivery vehicles.

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The effect of Lactobacillus casei extract on cervical cancer cell lines

Cited by 17 publications

References 20 publications

Potential of Maintaining a Healthy Vaginal Environment by TwoLactobacillusStrains Isolated from Cocoa Fermentation

Potential of Maintaining a Healthy Vaginal Environment by TwoLactobacillusStrains Isolated from Cocoa Fermentation

Immunotherapeutic efficacy of a Lactobacillus casei lysate as an adjuvant combined with a heated-4T1 mammary carcinoma cell lysate in a murine model of breast cancer

A polyethylene glycol-based method for extraction of extracellular vesicles from Lactobacillus casei as vaccine delivery vehicle

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