The Effect of Local Heating on the Flow and Composition of Rete Testis Fluid in the Conscious Ram

Setchell, B. P.; Voglmayr, J. K.; Hinks, N. T.

doi:10.1530/jrf.0.0240081

Cited by 42 publications

(30 citation statements)

References 17 publications

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“…Since in addition the ionic composition of RTF remains in the normal range throughout the onset of testicular damage caused by local heating in rams (Setchell, Voglmayr & Hinks, 1971) and rats (Setchell & Waites, 1972), a gross breakdown of the 'blood-testis barrier' cannot be the cause of heat-induced aspermatogenesis.…”

Section: Discussionmentioning

confidence: 99%

The blood-testis barrier and temperature damage to the testis of the rat

Main¹,

Waites²

1977

Reproduction

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Summary. The integrity of the blood\p=n-\testisbarrier was investigated during and after local heating of rat testes sufficient to produce a temporary cessation of spermatogenesis. The flow, ionic composition and protein content of rete testis fluid (RTF) collected from testes maintained at 33 or 41\s=deg\Cwere unaffected either at the time of treatment or up to 2 days later when the major cytological consequences of heating occurred. The normally low rate of transfer of albumin from blood to RTF was unaffected during and after heating. Transfer constants for radioactive K, Rb, Na and lysine consistently increased during heating although there were time-dependent differences between the patterns of response for each molecule. The normally rapid transfer of testosterone was unaffected by heating, but the entry rates of radioactivity into RTF after the infusion of more slowly diffusing steroids were enhanced at 41\s=deg\C. The clearest effects of heating were an approximate doubling in the uptake of oxygen and decrease in the net synthesis of protein by the testis. It is concluded that heating sufficient to damage spermatogenesis was not associated with dramatic alterations in the integrity of the blood\p=n-\ testis barrier but more with changes in testicular metabolism.

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Section: Discussionmentioning

confidence: 99%

The blood-testis barrier and temperature damage to the testis of the rat

Main¹,

Waites²

1977

Reproduction

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“…Sate.hell, Voglmayr and Hinks (1971) used the ca1;1.nulation of the rete · testi~ techt).ique to monitor testicular sperm . .…”

Section: Scrotal Insulation and Local Heatingmentioning

confidence: 99%

Evaluation of Epididymal Sperm by the Cannulation Technique and the Effects of In Vivo Storage in Angus Bulls

Deutscher

Wells

Battaglia

1974

Journal of Animal Science

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“…The levels of inositol increase as sperm travel from the testes to the epididymis, leading to the belief that inositol may play a role in spermatozoa maturation as they migrate from the testes (Eisenberg and Bolden, 1964). Moreover, the inositol concentration in the rete testis fluids of rams was also found to be positively correlated with spermatogenic activity (Setchell et al, 1971). In the present study, the testicular inositol level was found to have decreased in rats that had been exposed to ME, with or without Vit E co-treatment.…”

Section: Discussionmentioning

confidence: 99%

Metabolomic Analysis of the Effects of Motorcycle Exhaust on Rat Testes and Liver

Lin

Liang

et al. 2014

Aerosol Air Qual. Res.

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Motorcycles are one of the primary means of transportation in many Asian metropolitan areas. Exposure to motorcycle exhaust (ME) has been shown to cause male reproductive toxicity in a rat inhalation model. In the present study, the molecular mechanisms underlying ME-induced male reproductive toxicity were examined in a rat model using nuclear magnetic resonance (NMR)-based metabolomics. Rats were treated with 1:10-diluted ME for 1 hour in the morning and 1 hour in the afternoon, daily, via head-nose-only exposure chambers, for a period of four weeks. Half of the ME-exposed rats were co-treated with vitamin E to examine its protective effects. Both hydrophilic and hydrophobic metabolites from the testes and liver were extracted for 1 H and J-resolved NMR analyses. The obtained NMR spectra were converted into series of spectral binned areas and subjected to orthogonal projections to latent structure-discriminant analyses. Decreased spermatid counts and elevated levels of betaine in the testes of rats in the ME-treated group were partially attenuated by vitamin E co-treatment. Although it was not statistically significant, we observed a consistent trend of partial reversal of the elevated levels of alanine, glycine, leucine, tyrosine, and valine in the testes of rats in the ME-treated group due to vitamin E co-treatment. Decreases in testicular ethanolamine and inositol were only significant in the group co-treated with ME and vitamin E. The testicular toxicity of ME may be due to alterations in lipid-and energy-related metabolism. We conclude that ME generates greater metabolic responses in the testes than in the liver. Treatment with vitamin E can partially reverse the molecular events in the testes.

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The Effect of Local Heating on the Flow and Composition of Rete Testis Fluid in the Conscious Ram

Cited by 42 publications

References 17 publications

The blood-testis barrier and temperature damage to the testis of the rat

The blood-testis barrier and temperature damage to the testis of the rat

Evaluation of Epididymal Sperm by the Cannulation Technique and the Effects of In Vivo Storage in Angus Bulls

Metabolomic Analysis of the Effects of Motorcycle Exhaust on Rat Testes and Liver

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