The effect of low temperature and GA3 treatments on dormancy breaking and activity of antioxidant enzymes in Fritillaria meleagris bulblets cultured in vitro

Petrić, Marija; Jevremović, Slađana; Trifunović, Milana; Tadić, Vojin; Milošević, Snežana; Dragićević, Milan; Subotić, Angelina

doi:10.1007/s11738-013-1357-z

Cited by 19 publications

(39 citation statements)

References 64 publications

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“…This could be due to the higher duration of cold storage and clove topping, respectively, which resulted in earlier release of clove dormancy. Petric et al [26] also reported that GA3 can compensate the lack of low-temperature for specific time period, but not completely and Arifin et al [3] also reported the earlier dormancy breaking by cutting of bulbs.…”

Section: Effect Of Pre-planting Treatments On Sprouting Percentagementioning

confidence: 99%

“…The low sprouting percentage of non-cooled cloves suggests that the effect of gibberellic acid on these characteristics is weak unless the cloves were stored for a specific period of time at low temperatures. GA3 can compensate the lack of low-temperature for specific period of time, but not completely [26]. Certain amount of hydrolyzed sugar must be stored in the bulb during the minimum period of low temperatures, which in the case of Fritillaria meleagris L. bulb is 4 weeks, to have enough energy to sprout and grow after a dormancy break [26].…”

Section: Effect Of Pre-planting Treatments On Sprouting Percentagementioning

confidence: 99%

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Duration of low temperature storage, clove topping and gibberellic acid on garlic sprouting and seedling vigor

Desta¹,

Woldetsadik²,

Mohammed³

et al. 2018

AJFAND

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Dormancy of garlic (Allium sativum L.) creates a problem in use of freshly harvested garlic bulbs. Thus, pre-planting treatment of garlic cloves is an important agronomic concern for inducing and improving sprouting behavior of garlic. Glasshouse experiment was conducted at Haramaya University to evaluate the effect of cold storage (7°C) durations (10, 20, 30 days and one stored at room temperature that is 21°C for 30 days), clove topping (whole and cut) and gibberellic acid concentrations (0, 125, 250 and 375 mg/l) and distilled water treatment as second control on sprouting behavior of improved garlic variety ('Tseday'). The treatments were laid out in factorial arrangement with three replications using completely randomized design (CRD). For cloves stored at ambient temperature (0 day) and non-topped, gibberellic acid application (250 and 375 mg/l) showed a significant increase in sprouting percentage, speed of sprouting and shoot dry mass of garlic as compared to 125mg/l GA3 treated and the controls. On the other hand, for topped cloves stored at ambient temperature (0 day), 10 and 20 days cold storage, gibberellic acid treatment did not show a significant influence on these characteristics as compared to the controls. The interaction effect of 30 days cold stored and topped cloves significantly increased pseudo-stem height (40.51%) compared to the lowest values recorded for the non-cold treated and non-topped cloves. The 30 days cold stored and topped cloves soaked in water significantly increased sprouting percentage over the period of 19 days after planting, speed of sprouting (four fold) and above ground shoot dry mass (79.41%) compared to the lowest values recorded for the non-cold treated and non-topped cloves not soaked in water and gibberellic acid (GA3). Hence, it can be concluded that 30 days cold storage, topping and soaking in water could enhance early sprouting of the garlic variety tested under glasshouse condition.

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Section: Effect Of Pre-planting Treatments On Sprouting Percentagementioning

confidence: 99%

Section: Effect Of Pre-planting Treatments On Sprouting Percentagementioning

confidence: 99%

Duration of low temperature storage, clove topping and gibberellic acid on garlic sprouting and seedling vigor

Desta¹,

Woldetsadik²,

Mohammed³

et al. 2018

AJFAND

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“…Gibberellins have been known to stimulate growth, and have a dormancy terminating capacity in potato [46,47]. Umrao et al [48] and Petric et al [49] also reported that the sprouting of bulbs and corms can be hastened with gibberellin treatment.…”

Section: In Vivo Propagation Of Vegetative Propagulesmentioning

confidence: 99%

Conservation and Cultivation of Ferula jaeschkeana Vatke: A Species with Deep Complex Morphophysiological Dormancy

Yaqoob

Nawchoo

2015

Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci.

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Ferula jaeschkeana Vatke, an important medicinal plant of the Himalayan region, being threatened needs immediate conservation strategies. Keeping this in view studies on the germination of seeds and sprouting of vegetative propagules has been undertaken for the first time. At the time of dispersal, F. jaeschkeana seeds are dormant and have under-developed embryos. The aims of the present work were to determine the requirements for germination and overcoming dormancy and to characterize the type of seed dormancy. The seeds of F. jaeschkeana have deep complex morpho-physiological dormancy. Present study revealed that final germination percentage, mean daily germination, seed vigour index, germination index, germination rate and germination value were highest (53.3 %, 0.88, 3.67, 0.13, 0.88 and 1.02, respectively) when 200 ppm kinetin and dry stratification (60 days) were applied together. The decline in MGT up to 36.18 days and highest percentage survival (87.9 ± 7.08) were also observed in the same treatment. This treatment is thus recommended for promoting the germination process of F. jaeschkeana seeds. Maximum seed germination (62.0 ± 11.54 %) at 1 in. depth was found in seeds presoaked in kinetin (100 ppm) for 24 h in media of sand:soil (2:1). In case of root tuber cuttings, 500 ppm gibberellic acid showed significantly high sprouting (89.70 ± 0.78 and 69.14 ± 0.82 %, respectively) and rooting percentage (78.78 ± 1.13 and 55.56 ± 0.95 %, respectively) in case of both perennial and biennial plants. Likewise minimum time taken for sprouting was also observed in the same treatment in case of both perennial (10.0 ± 1 days) and biennial plants (11.33 ± 2.3 days).

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“…Chlorophyll fluorescence analysis is one of the most powerful and popular techniques for the investigation of the influence of various stresses on the leaf gas exchange [23]. Several studies reported that LT significantly reduced net photosynthesis rate (Pn) and the ratio of variable to maximal fluorescence (Fv/Fm) [24][25][26][27] in plants; it also increased the activity of antioxidant enzymes such as superoxide dismutase (SOD, EC 1.15.1.1) and catalase (CAT, EC 1.11.1.6) [28] to resist possible injury. However, if the production rate of reactive oxygen species exceeds the scavenging ability of antioxidants, reactive oxygen species would excessively accumulate and damage plant tissues [29][30].…”

Section: Introductionmentioning

confidence: 99%

Physiological and transcriptomic responses of Lanzhou Lily (Lilium davidii, var. unicolor) to cold stress

Tian

Xie

2020

PLoS ONE

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Low temperature induces changes in plants at physiological and molecular levels, thus affecting growth and development. The Lanzhou lily (Lilium davidii, var. unicolor) is an important medicinal plant with high economic value. However, the molecular mechanisms underlying its photosynthetic and antioxidation responses to low temperature still remain poorly understood. This study subjected the Lanzhou lily to the two temperatures of 20˚C (control) and 4˚C (low temperature) for 24 h. Physiological parameters related to membrane integrity, photosynthesis, antioxidant system, and differentially expressed genes were investigated. Compared with control, low temperature increased the relative electrical conductivity by 43.2%, while it decreased net photosynthesis rate, ratio of variable to maximal fluorescence, and catalase activity by 47.3%, 10.1%, and 11.1%, respectively. In addition, low temperature significantly increased the content of soluble protein, soluble sugar, and proline, as well as the activity of superoxide dismutase and peroxidase. Comparative transcriptome profiling showed that a total of 238,109 differentially expressed genes were detected. Among these, 3,566 were significantly upregulated while 2,982 were significantly downregulated in response to low temperature. Gene Ontology enrichment analysis indicated that in response to low temperature, the mostly significantly enriched differentially expressed genes were mainly involved in phosphorylation, membrane and protein kinase activity, as well as photosynthesis, light harvesting, light reaction, and alpha,alpha-trehalose-phosphate synthase activity. Kyoto Encyclopedia of Genes and Genomes enrichment analysis also indicated that the most significantly enriched pathways involved ribosome biogenesis in eukaryotes, phenylalanine metabolism, circadian rhythm, porphyrin and chlorophyll metabolism, photosynthesis of antenna proteins, photosynthesis, and carbon fixation in photosynthetic organisms. Moreover, the expression patterns of 10 randomly selected differentially expressed genes confirmed the RNA-Seq results. These results expand the understanding of the physiological and molecular mechanisms underlying the response of the Lanzhou lily to low temperature stress.

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The effect of low temperature and GA3 treatments on dormancy breaking and activity of antioxidant enzymes in Fritillaria meleagris bulblets cultured in vitro

Cited by 19 publications

References 64 publications

Duration of low temperature storage, clove topping and gibberellic acid on garlic sprouting and seedling vigor

Duration of low temperature storage, clove topping and gibberellic acid on garlic sprouting and seedling vigor

Conservation and Cultivation of Ferula jaeschkeana Vatke: A Species with Deep Complex Morphophysiological Dormancy

Physiological and transcriptomic responses of Lanzhou Lily (Lilium davidii, var. unicolor) to cold stress

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