The effect of Mesenchymal stem cells- derived exosomes on the expression of MAGEA6 and MAGEA11 in a human colorectal carcinoma cell line

Abstract

Background: Melanoma Antigen Gene (MAGE) proteins belong to a large, highly conserved family of proteins with a common homology domain. Most MAGE proteins are expressed exclusively in reproductive tissues, but they are aberrantly expressed in many types of cancer. In this study, we aimed to investigate the effects of adipose-derived mesenchymal stem cells secreted exosomes on the expression of MAGEA6 and MAGEA11 genes in the HCT-116 tumor cell line. Materials and Methods: Ad-MSCs were assessed for their surface antigenic profile using specific markers. TEM and western blot were used to evaluate the quality of the isolated exosomes, which were purified from the Ad-MSc supernatant. HCT-116 cells were co-cultured with MSC-conditioned medium (MSC-CM) and/or with 100 ug/ml of MSC-derived exosomes for 48h. Real-time PCR was carried out to determine the expression of MAGEA6 and MAGEA11 in HCT-116. Relative expression levels were calculated using the 2-ΔΔct method. Results: Our result showed that MAGEA11 mRNA expression levels were significantly reduced in exosome (EXO) and/or CM (MSC- conditioned medium) +EXO treated HCT116 while MAGEA6 mRNA expression levels were significantly reduced in CM+EXO treated HCT116 (P-value < 0.05). Conclusion: The current study showed that MSC-derived exosomes could inhibit the expression of two important molecules involved in tumor progression. Hence it seems MSCs-derived exosomes may hold a hopeful future as drug delivery vehicles that need further investigation.