The effect of mesenchymal stem cell-conditioned medium gel on burn wound healing in rat

Abstract

Background and Aim: Stem cells are cells that can proliferate to form a new tissue, leading to its use in regenerative therapy. Stem cells will secrete biological factors, such as growth factors, cytokines, and other proteins to their surroundings and culture medium/conditioned medium (CM), altering tissue physiology. These factors can help wound healing, but their effect on third-degree burns is poorly understood. This research aimed to study the activity of mesenchymal stem cell-conditioned medium gel in healing and repairing third-degree burns on rats skin. Materials and Methods: Twenty-four Sprague–Dawley rats with burn wounds on the dorsal area were divided into four groups; the first group was treated with CM gel, with a concentration equivalent to 0.05% protein, the second group was treated with a placebo gel, the third group with silver sulfadiazine (SSD) cream (SSD-Burnazin contain 10 mg/g SSD), and the fourth group was not given any treatment, for 21 days, and on the final day, the rats were sacrificed, and the skins were taken. All topical treatments completely cover the wound area. Results: Wound healing process indicators observed include wound diameter, scabs' formation, blister formation, and hair growth every day. The skins taken were processed with hematoxylin-eosin and Masson's trichrome staining. The indicators studied include neutrophil infiltration, mononuclear cell infiltration, neovascularization, collagen area, and re-epithelization ratio. Conclusion: CM shows better wound healing than other groups and faster hair growth.

Bone marrow-derived mesenchymal stem cell-conditioned medium ameliorates diabetic foot ulcers in rats

“… 39 A recent study showed that adipose-derived stem cell CM could accelerate wound healing and hair growth in SD rats with burn wounds on the dorsal, but this study also did not reveal the mechanism of the treatment. 40 In the present study, HE and Ki67 staining suggested that the treatment of MSC-CM promoted the proliferation of skin tissue, CD31 staining suggested that the treatment promoted the proliferation of blood vessels and increased the local blood supply. Electron microscopy showed that the treatment promoted cell autophagy.…”

In Vivo Incision Wound Healing Studies using Ethanolic Cinnamon’s (Cinnamomum burmannii) Leaves Extract in White Male Rats

“…Besides this, results use a measurement of the hydroxyproline levels as an index of collagen turnover (28) . The results show a signifi cant diff erence in hydroxyproline levels for the ethanol extract of cinnamon leaves and indicate an increase in breaking down granulation tissue from collagen maturation in injured skin (29,30) . CONCLUSION…”

Secretome of bovine umbilical vein endothelial cells promote wound healing regeneration on the second degree rat model burn injury

“…This research calculated the sample size using “Power and Sample Size Calculation” application version 3.2.6 (Informer Technologies, Inc.). In previous studies, the average wound closure treated with MSC-CM was 67.03%, while that of the controls was 52.18% ( Laksmitawati et al ., 2022 ). With an alpha of 0.01, a power of 0.90, an SD of 8.38, and a difference of 15%, the required sample is 11 tails plus one tail for reserve.…”