The effect of metronidazole releasing polymer coatings on in vitro biofilm formation

Abstract

Abstract:Background: The aim of the study was to evaluate the antibacterial effect of a drug releasing poly (3-hydroxybutyrate) (P(3HB)) implant coating in comparison to pure titanium on Aggregatibacter actinomycetemcomitans as a model periodontopathogen to prevent biofilm formation on implant surfaces. Methods: Titanium discs were coated with P(3HB) containing 5% (w) and 10% (w) of metronidazole, either with and without a P(3HB) topcoat. The biofilm formation was evaluated after 1, 4 and 9 days in a dynamic flow chamber system. Microbial adherence was quantified by determination of bacterial surface coverage. Results: The evaluated formulations of P(3HB)/metronidazole showed an antibacterial effect especially in the first 24 h. Prolonged incubation for 9 days showed reduced bacterial adhesion only on polymer coatings loaded with 10% (w) of metronidazole both with and without topcoat. Conclusions:The evaluated coating formulations can provide protection from an Aggregatibacter actinomycetemcomitans in vitro biofilm formation for the time period which was evaluated.

Biofunctionalization

“…A review of PEEK modification reactions are collocated with general information on polymer-based drug delivery systems as well as the biofunctionalization of polymers and with a discussion of their applicability for PEEK in the article by Harting et al [6]. Freifrau von Maltzahn et al [7] in contrast highlights the evaluation of the antibacterial effect of a drug releasing poly(3-hydroxybutyrate) [P(3HB)] implant coating in comparison to pure titanium on Aggregatibacter actinomycetemcomitans as a model periodontopathogen to prevent biofilm formation on dental implant surfaces. A further modification possibility is the alteration of surface roughness of dental implant surfaces.…”