The effect of molybdenum and nitrogen deficiencies on nitrate reduction in plant tissues

Mulder, E. G.; Boxma, R.; Veen, W. L. van

doi:10.1007/bf01666209

Cited by 94 publications

(37 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A atividade da redutase do nitrato foi determinada, de acordo com metodologia descrita por Mulder et al (1959). No estádio 10.5 do trigo (aos 79 dias de cultivo) foram coletadas amostras do terço médio da lâmina da primeira folha abaixo da folha bandeira de uma planta por vaso.…”

Section: Methodsunclassified

Doses de nitrogênio e potássio no crescimento do trigo

Viana

Kiehl

2010

Bragantia

View full text Add to dashboard Cite

RESUMOO fornecimento do potássio para as plantas estimula o aproveitamento do nitrogênio, possibilitando que sua absorção, assimilação, nutrição e, consequentemente, a produtividade, sejam aumentadas. Objetivou-se com este trabalho avaliar o efeito de combinações de doses de nitrogênio e potássio no crescimento e assimilação do nitrogênio por plantas de trigo. O experimento foi realizado em casa de vegetação, utilizando-se vasos com capacidade para 3 kg onde foram acondicionadas amostras da camada 0-0,2 m de um Argissolo Vermelho Amarelo Abrúptico. Utilizou-se um esquema fatorial 5 2 fracionado, com combinações de cinco doses de nitrogênio (0; 70; 140; 210 e 280 mg dm -3 ) e de cinco doses de potássio (0; 50; 100; 150 e 200 mg dm -3 ), as quais foram distribuídas segundo o delineamento experimental de blocos aleatorizados, com quatro repetições. O fornecimento combinado das maiores doses de nitrogênio e potássio incrementou a produção de massa seca da parte aérea e teor de clorofila nas folhas. O acúmulo de nitrogênio e potássio nas folhas foi aumentado apenas pelas doses desses nutrientes separadamente. O potássio não influenciou a atividade da redutase do nitrato e o acúmulo de nitrogênio no tecido vegetal. Palavras-chave:Triticum aestivum L., atividade da enzima redutase do nitrato, leituras SPAD. ABSTRACT RATES OF NITROGEN AND POTASSIUM ON GROWTH OF WHEAT PLANTSThe supply of potassium to plants stimulates the efficient use of nitrogen through plant uptake and assimilation, consequently increasing crop yield. The aim was to evaluate the effect of combined rates of nitrogen and potassium on growth and assimilation of nitrogen in wheat plants. The experiment was performed in a greenhouse, Piracicaba, SP Brazil. Three-liter pots were filled with 0-0.2 m depth samples of Arenic Hapludult. A fractionated 5 2 factorial was used, with combinations of five nitrogen rates (0; 70; 140; 210 and 280 mg dm -3 ) and five potassium rates (0; 50; 100; 150 and 200 mg dm -3 ), which were set in a randomized block design, with four replications. The combination of the highest nitrogen and potassium rates increased shoot growth and the chlorophyll content in leaves. The accumulation of nitrogen and potassium in leaves was increased only by rates of these nutrients separately. The potassium did not influence the nitrate reductase activity and the accumulation of nitrogen in plant tissue.

show abstract

Section: Methodsunclassified

Doses de nitrogênio e potássio no crescimento do trigo

Viana

Kiehl

2010

Bragantia

View full text Add to dashboard Cite

show abstract

“…(That is, the migration of the phosphorylated sugars into the cytoplasm would provide the substrates necessary for the generation of NADH by the predominantly cytoplasmically located NAD-dependent 3-PGAId dehydrogenase [16,32,39]). The development of an in vivo assay for nitrate reductase in chlorophyllous (24,29,33) …”

mentioning

confidence: 99%

“…The development of an in vivo assay for nitrate reductase in chlorophyllous (24,29,33) and nonchlorophyllous tissue (12) provides an experimental approach for verification that photosynthate is the source of energy for nitrate reduction. Specific objectives were: (a) to determine the metabolic pathway or system responsible for generation of NADH required for nitrate reduction; (b) to identify the specific enzyme and were harvested from mature plants grown in fertile soil in a greenhouse (no supplemental lighting) or from young plants grown in a fertile garden, respectively.…”

mentioning

confidence: 99%

Generation of Reduced Nicotinamide Adenine Dinucleotide for Nitrate Reduction in Green Leaves

1971

View full text Add to dashboard Cite

An in vivo assay of nitrate reductase activity was developed by vacuum infiltration of leaf discs or sections with a solution of 0.2 M KNO3 (with or without phosphate buffer, pH 7.5) and incubation of the infiltrated tissue and medium under essentially anaerobic conditions in the dark. Nitrite production, for computing enzyme activity, was determined on aliquots of the incubation media, removed at intervals.By adding, separately, various metabolites of the glycolytic, pentose phosphate, and citric acid pathways to the infiltrating media, it was possible to use the in vivo assay to determine the prime source of reduced nicotinamide adenine dinucleotide (NADH) required by the cytoplasmically located NADH-specific nitrate reductase. It was concluded that sugars that migrate from the chloroplast to the cytoplasm were the prime source of energy and that the oxidation of glyceraldehyde 3-phosphate was ultimately the in vivo source of NADH for nitrate reduction.This conclusion was supported by experiments that included: inhibition studies with iodoacetate; in vitro studies that established the presence and functionality of the requisite enzymes; and studies showing the effect of light (photosynthate) and exogenous carbohydrate on loss of endogenous nitrate from plant tissue.The level of nitrate reductase activity obtained with the in vitro assay is higher (2.5-to 20-fold) than with the in vivo assay for most plant species. The work done to date would indicate that the in vivo assays are proportional to the in vitro assays with respect to ranking genotypes for nitrate-reducing potential of a given species. The in vivo assay is especially useful in studying nitrate assimilation in species like giant ragweed from which onlv traces of active nitrate reductase can be extracted.indirectly via oxidation of carbohydrates produced photosynthetically. Work with illuminated algae and Perilla (42,9,20) suggests that carbohydrate oxidation is directly involved in nitrate reduction and thus would be similar to the process operative in nonchlorophyllous tissue (45). In contrast, Mendel and Visser (28) deduced from studies with 15N-nitrate using tomato leaves that nitrate reduction and assimilation was dependent on respiratory (mitochondrial) energy in the dark while a separate light-dependent pathway was operative with illumination. The isolation of a NAD(P)H-nitrate reductase from soybean leaves and the demonstration that a reconstituted system of enzyme, grana, and NADP when illuminated would reduce nitrate seemed to establish the coupling of light via an electron transport system to nitrate reduction (11). Doubt was cast upon this conclusion by the subsequent findings that nitrate reductase in leaf tissue (including soybeans) is NADHdependent (2, 3, 44), nitrate reductase is localized in the cytoplasm and not within the chloroplasts (34, 36), and pyridine nucleotides do not readily migrate from the chloroplasts (18,31). In contrast, several photosynthetic metabolites (3-PGA, 3-PGAId, 3-DHAP, and RDP are most mobile)3 mig...

show abstract

“…A determinação indireta do teor de clorofila (valores SPAD), com uso do Chlorophyll Meter SPAD-502 (MINOLTA CAMERA Co., 1989), a determinação das concentrações de N, Ca, K, Mg e S e a atividade da redutase do nitrato (MULDER; BOXMA; VAN VENN, 1959) foram efetuadas no lóbulo mediano da terceira e quarta folha superior recém-expandida (LAVRES JUNIOR et al, 2005), aos 56 dias após o início dos tratamentos (DAT). Determinou-se a área foliar, contabilizando somente as folhas verdes e aquelas em início de senescência, na ocasião do corte das plantas (aos 126 DAT), por meio de um sistema digital, medidor de área foliar LI-3100…”

Section: Methodsunclassified

Biomass yield, macronutrient diagnosis, and nitrogen and calcium uptake during early growth of physic nut

Garrone

Campos

Silveira

et al. 2016

Revista Ciência Agronômica

View full text Add to dashboard Cite

* RESUMO -As maiores exigências em nitrogênio (N) e cálcio (Ca) têm sido relatadas para as espécies da família Euforbiaceae. Objetivou-se avaliar o suprimento combinado de doses de N e Ca e seu efeito na avaliação do estado nutricional da Jatropha curcas, por meio das diagnoses visual, foliar e bioquímica (redutase do nitrato RNO 3 -e o teor de clorofila), além de determinar o rendimento de biomassa e os acúmulos de N e Ca durante o crescimento inicial das plantas. Foram utilizadas as doses 2; 9; 16; 23 e 30 mmol L -1 de N e 0,5; 1,75; 3,0; 4,25 e 5,5 mmol L -1 de Ca na solução nutritiva. As concentrações de N, Ca e K, nas folhas utilizadas para diagnose, foram alteradas pelas combinações de doses de N e Ca, enquanto que as concentrações de Mg foram alteradas somente pelas doses de Ca. O aumento das concentrações de N e Ca na solução resultou em incrementos na área foliar e na produção de biomassa de folhas em 400% e 245%, respectivamente. Em média, as quantidades de N e Ca acumuladas nas folhas corresponderam a 29% e 39% do total acumulado nas plantas. O rendimento de biomassa guarda relação direta com a atividade da RNO 3 -e teor de clorofila, sendo a faixa de concentração de N nestas folhas de 43 a 45 g kg -1 para a máxima produção. O nitrogênio promove maior impacto no rendimento de biomassa das plantas, todavia, os efeitos são mais expressivos com o aumento da absorção de Ca.Palavras-chave: Área foliar. Diagnose foliar. Jatropha curcas. Redutase do nitrato.ABSTRACT -Higher nitrogen (N) and calcium (Ca) requirements than other macronutrients in Euphorbiaceous species have been pointed out in the literature. Thus, the objective of this work was evaluate the effects of N and Ca combination rates on the visual deficiencies of both nutrients, on the macronutrient status, chlorophyll content and nitrate reductase activity (RNO 3 -) taken on the recent expanded leaves of Jatropha curcas, as well as to evaluate N and Ca uptake and biomass yield during early plant growth, subjected to 2; 9; 16; 23 e 30 mmol L -1 N rates and 0.5; 1.75; 3.0; 4.25 e 5.5 mmol L -1 Ca rates, in the nutrient solution. Nitrogen, Ca and K concentrations on the recent expanded leaves were changed by N and Ca rates, whereas leaf-Mg concentrations ranged only by Ca supply. Increasing N and Ca rates in solution led to increased leaf area and the leaf biomass yield by 400% and 245%, respectively. On average, N and Ca taken up on leaves accounted for 29% and 39% of the plant uptake. Biomass yield is directly related to RNO 3 -activity and chlorophyll content, and the range of N concentration in these leaves related to highest plant dry matter production is 43-45 g kg -1. In conclusion, N supply causes greater impact on the plant biomass yield; however, the effects are more relevant with increased Ca uptake.

show abstract

The effect of molybdenum and nitrogen deficiencies on nitrate reduction in plant tissues

Cited by 94 publications

References 10 publications

Doses de nitrogênio e potássio no crescimento do trigo

Doses de nitrogênio e potássio no crescimento do trigo

Generation of Reduced Nicotinamide Adenine Dinucleotide for Nitrate Reduction in Green Leaves

Biomass yield, macronutrient diagnosis, and nitrogen and calcium uptake during early growth of physic nut

Contact Info

Product

Resources

About