The effect of okadaic acid on meiotic maturation of canine oocytes of different size

Ariu, Federica; Fois, Stefano; Bebbere, Daniela; Ledda, Sergio; Rosati, Irma; Zedda, Maria Teresa; Pau, Salvatore; Bogliolo, Luisa

doi:10.1016/j.theriogenology.2011.07.013

Cited by 8 publications

(4 citation statements)

References 26 publications

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“…Together with these studies, OA was proven by two independent bioassays to exhibit a weak teratogenicity on cultured murine embryonic cells even at a 5 nM concentration [115], to increase the meiotic resumption and maturation rates of canine oocytes [116], to affect meiotic resumption of blue fox oocytes in vitro [117], and to resume meiosis with fast kinetics of germinal vesicle breakdown through the MEK-MAPK pathway in incompetent growing mouse oocytes [118]. In addition, OA was found to induce premature chromosome condensation, meiosis resumption, pronucleus breakdown, inhibition of spindle organization, and microtubule assembly suppression by sperm centrosomes in porcine oocytes and fertilized eggs [119].…”

Section: Oa Beyond Its Role As Dsp Inductormentioning

confidence: 99%

Okadaic Acid: More than a Diarrheic Toxin

et al. 2013

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Okadaic acid (OA) is one of the most frequent and worldwide distributed marine toxins. It is easily accumulated by shellfish, mainly bivalve mollusks and fish, and, subsequently, can be consumed by humans causing alimentary intoxications. OA is the main representative diarrheic shellfish poisoning (DSP) toxin and its ingestion induces gastrointestinal symptoms, although it is not considered lethal. At the molecular level, OA is a specific inhibitor of several types of serine/threonine protein phosphatases and a tumor promoter in animal carcinogenesis experiments. In the last few decades, the potential toxic effects of OA, beyond its role as a DSP toxin, have been investigated in a number of studies. Alterations in DNA and cellular components, as well as effects on immune and nervous system, and even on embryonic development, have been increasingly reported. In this manuscript, results from all these studies are compiled and reviewed to clarify the role of this toxin not only as a DSP inductor but also as cause of alterations at the cellular and molecular levels, and to highlight the relevance of biomonitoring its effects on human health. Despite further investigations are required to elucidate OA mechanisms of action, toxicokinetics, and harmful effects, there are enough evidences illustrating its toxicity, not related to DSP induction, and, consequently, supporting a revision of the current regulation on OA levels in food.

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Section: Oa Beyond Its Role As Dsp Inductormentioning

confidence: 99%

Okadaic Acid: More than a Diarrheic Toxin

et al. 2013

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“…To prevent DSP, the European Union implemented a limit of 160 OA equivalents/kg shellfish meat, based on acute toxic effects, such as diarrhea [3]. Moreover, there are multiple reported properties of OA, such as cytotoxicity [4][5][6] and embryotoxicity in vitro [7,8]. However, no embryotoxic effect of OA in humans has been evaluated so far.…”

Section: Introductionmentioning

confidence: 99%

Okadaic Acid Activates JAK/STAT Signaling to Affect Xenobiotic Metabolism in HepaRG Cells

Wuerger

Kudiabor

Alarcan

et al. 2023

Cells

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Okadaic acid (OA) is a marine biotoxin that is produced by algae and accumulates in filter-feeding shellfish, through which it enters the human food chain, leading to diarrheic shellfish poisoning (DSP) after ingestion. Furthermore, additional effects of OA have been observed, such as cytotoxicity. Additionally, a strong downregulation of the expression of xenobiotic-metabolizing enzymes in the liver can be observed. The underlying mechanisms of this, however, remain to be examined. In this study, we investigated a possible underlying mechanism of the downregulation of cytochrome P450 (CYP) enzymes and the nuclear receptors pregnane X receptor (PXR) and retinoid-X-receptor alpha (RXRα) by OA through NF-κB and subsequent JAK/STAT activation in human HepaRG hepatocarcinoma cells. Our data suggest an activation of NF-κB signaling and subsequent expression and release of interleukins, which then activate JAK-dependent signaling and thus STAT3. Moreover, using the NF-κB inhibitors JSH-23 and Methysticin and the JAK inhibitors Decernotinib and Tofacitinib, we were also able to demonstrate a connection between OA-induced NF-κB and JAK signaling and the downregulation of CYP enzymes. Overall, we provide clear evidence that the effect of OA on the expression of CYP enzymes in HepaRG cells is regulated through NF-κB and subsequent JAK signaling.

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“…The collection and processing of samples was performed according to that previously published by Ariu et al . (). Briefly, ovaries ( n = 28) were collected from bitches (aged 8 months to 6 years) at different stages of the estrous cycle undergoing routine ovariohysterectomy in the Veterinary Hospital of the University of Sassari, Italy.…”

Section: Methodsmentioning

confidence: 97%

“…Oocytes were completely denuded via gentle pipetting with a fine bore glass pipette, selected on the basis of the phase of the oestrous cycle and divided according to their diameter, zona pellucida excluded, as previously described by Ariu et al . (), in two homogeneous groups: 110‐120 µm, >120 µm.…”

Section: Methodsmentioning

confidence: 99%

Lipid droplet distribution of immature canine oocytes in relation to their size and the reproductive stage

Ariu

Strina

Murrone

et al. 2015

Animal Science Journal

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This study investigated the distribution of lipid droplets (LD) in immature canine oocytes in relation to their size and the reproductive stage. Oocytes were collected from the ovaries of bitches at different estrous stages, divided according to their size (110-120 µm; >120 µm), and stained with Nile Red to detect lipid droplet distribution. At the follicular phase most of the oocytes displayed a diffuse pattern of LD distribution, whereas at anestrus and luteal phase oocytes showed LD mainly in a peripheral/ perinuclear LD distribution. A significantly higher intensity of LD has been recorded in the oocytes > 120 µm compared to those of smaller size (110 - 120 µm) at all stages of the estrous cycle. At follicular phase, oocytes > 120 µm displayed LD intensity similar to that of oocytes > 120 µm at luteal phase and higher compared to the oocytes of the other groups.

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The effect of okadaic acid on meiotic maturation of canine oocytes of different size

Cited by 8 publications

References 26 publications

Okadaic Acid: More than a Diarrheic Toxin

Okadaic Acid: More than a Diarrheic Toxin

Okadaic Acid Activates JAK/STAT Signaling to Affect Xenobiotic Metabolism in HepaRG Cells

Lipid droplet distribution of immature canine oocytes in relation to their size and the reproductive stage

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