The effect of pacific cod (Gadus macrocephalus) skin gelatin polypeptides on UV radiation-induced skin photoaging in ICR mice

Hou, Hu; Li, Bafang; Zhao, Xue; Zhuang, Yongliang; Ren, Guoyan; Yan, Mingyan; Cai, Yi; Xiu-kun, Zhang; Chen, Li

doi:10.1016/j.foodchem.2009.01.015

Cited by 97 publications

(62 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Small amounts of cysteine (0.01%) and methionine (1.77%) were identified in the gelatin, similarly to what was reported by Hou et al (2009). These amino acids play a crucial role in the formation of disulfide bonds (Foegeding et al, 1996).…”

Section: Characterization Of the Skin And Gelatinsupporting

confidence: 77%

Gelatin extraction from Kumakuma (Brachyplathystoma filamentosum) skin using the liming method

Silva¹,

Pena²,

Lourenço³

2016

Afr. J. Agric. Res.

View full text Add to dashboard Cite

Gelatin extraction process from Kumakuma (Brachyplathystoma filamentosum) skin was optimized using a calcium hydroxide solution. The gelatin obtained was characterized through scanning electron microscopy and analyses of yield, gel strength, color, viscosity, amino acid profile, melting point, foaming capacity, and emulsifying capacity. The optimized conditions were defined over ten days of pre-treatment at 50°C. This condition resulted in desirability of 0.965 and yield and gel strength values of 20.24% and 221 g, respectively. Glycine was the main amino acid both in the fish skin (11.68%) and in the gelatin obtained (23.39%). Gelatin had extendable and consistent gel characteristics and its microstructure showed even threads with small gaps throughout, which is favorable for the food industry.

show abstract

Section: Characterization Of the Skin And Gelatinsupporting

confidence: 77%

Gelatin extraction from Kumakuma (Brachyplathystoma filamentosum) skin using the liming method

Silva¹,

Pena²,

Lourenço³

2016

Afr. J. Agric. Res.

View full text Add to dashboard Cite

show abstract

“…The largest population A 22 (Table 1, Figure 1b) showed a significant decrease from 2376.58 to 982.71, which demonstrated the entrapped water loss because of degradation of protein and fracture of muscle fibers. As for A 23 (Table 1, Figure 1d), the area increased first and then decreased, revealing that the free water in extra-myofibrillar cells varied at different freezing-thawing cycles. With the development of NMR technology, MRI has gained increasing attention for its rapid, direct and accurate imaging ability.…”

Section: Nmr and Mri Analysis Of Turbot Flesh During Freezing-thawingmentioning

confidence: 80%

“…All the small frozen sections were stained with the V-G (Van Gieson) method [23] to observe the muscle tissue structure. Three representative point in time was chosen to detect the microstructure changes during the freezing-thawing process including initial stage (raw flesh), intermediate stage (15th freezing-thawing cycle) and final stage (30th freezing-thawing cycle), respectively.…”

Section: Optical Microscopementioning

confidence: 99%

Influence of Freezing–Thawing Cycle on Water Dynamics of Turbot Flesh Assessed by Low-Field Nuclear Magnetic Resonance and Magnetic Resonance Imaging

Xia

Yao

et al. 2018

International Journal of Food Engineering

View full text Add to dashboard Cite

Abstract:Turbot is a valuable commercial species due to its high nutrient content. Moisture is an important indicator of meat spoilage. This study elucidated distinctive water dynamics in turbot flesh in the freezing-thawing process by nondestructive low-field nuclear magnetic resonance (LF-NMR) and magnetic resonance imaging (MRI) techniques. T 2 relaxation spectra were utilized to describe the mobility and content of different types of water. Principal component analysis (PCA) revealed a clear discrimination of various freezing-thawing cycles. T 1 -and T 2 -weighted MRI provided further visualization of internal information for turbot flesh. Microscopic examination clearly identified protein denaturation and structural shrinkage. Furthermore, NMR parameters and conventional physicochemical parameters of color, shear force and thiobarbituric acid-reactive substances showed good correlations. To sum up, the study revealed that LF-NMR and MRI are promising techniques to portray the relationship between the water dynamics and changes of turbot quality properties during the freezing-thawing process.

show abstract

“…The activity of CAT was measured using a CAT detection kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) according to the manufacturer's instructions. CAT activity was measured using the ammonium molybdate spectrophotometric method, which is based on the fact that ammonium molybdate can rapidly terminate the H 2 O 2 degradation reaction catalyzed by CAT and react with the residual H 2 O 2 to generate a yellow complex, which could be monitored by the absorbance at 405 nm (30). All experiments were performed in triplicate.…”

Section: Methodsmentioning

confidence: 99%

Expression of the Laccase Gene from a White Rot Fungus in Pichia pastoris Can Enhance the Resistance of This Yeast to H ₂ O ₂ -Mediated Oxidative Stress by Stimulating the Glutathione-Based Antioxidative System

Yang

Fan

Zhuo

et al. 2012

Appl Environ Microbiol

View full text Add to dashboard Cite

ABSTRACTLaccase is a copper-containing polyphenol oxidase that has great potential in industrial and biotechnological applications. Previous research has suggested that fungal laccase may be involved in the defense against oxidative stress, but there is little direct evidence supporting this hypothesis, and the mechanism by which laccase protects cells from oxidative stress also remains unclear. Here, we report that the expression of the laccase gene from white rot fungus inPichia pastoriscan significantly enhance the resistance of yeast to H2O2-mediated oxidative stress. The expression of laccase in yeast was found to confer a strong ability to scavenge intracellular H2O2and to protect cells from lipid oxidative damage. The mechanism by which laccase gene expression increases resistance to oxidative stress was then investigated further. We found that laccase gene expression inPichia pastoriscould increase the level of glutathione-based antioxidative activity, including the intracellular glutathione levels and the enzymatic activity of glutathione peroxidase, glutathione reductase, and γ-glutamylcysteine synthetase. The transcription of the laccase gene inPichia pastoriswas found to be enhanced by the oxidative stress caused by exogenous H2O2. The stimulation of laccase gene expression in response to exogenous H2O2stress further contributed to the transcriptional induction of the genes involved in the glutathione-dependent antioxidative system, includingPpYAP1,PpGPX1,PpPMP20,PpGLR1, andPpGSH1. Taken together, these results suggest that the expression of the laccase gene inPichia pastoriscan enhance the resistance of yeast to H2O2-mediated oxidative stress by stimulating the glutathione-based antioxidative system to protect the cell from oxidative damage.

show abstract

The effect of pacific cod (Gadus macrocephalus) skin gelatin polypeptides on UV radiation-induced skin photoaging in ICR mice

Cited by 97 publications

References 27 publications

Gelatin extraction from Kumakuma (Brachyplathystoma filamentosum) skin using the liming method

Gelatin extraction from Kumakuma (Brachyplathystoma filamentosum) skin using the liming method

Influence of Freezing–Thawing Cycle on Water Dynamics of Turbot Flesh Assessed by Low-Field Nuclear Magnetic Resonance and Magnetic Resonance Imaging

Expression of the Laccase Gene from a White Rot Fungus in Pichia pastoris Can Enhance the Resistance of This Yeast to H ₂ O ₂ -Mediated Oxidative Stress by Stimulating the Glutathione-Based Antioxidative System

Contact Info

Product

Resources

About

The effect of pacific cod (Gadus macrocephalus) skin gelatin polypeptides on UV radiation-induced skin photoaging in ICR mice

Cited by 97 publications

References 27 publications

Gelatin extraction from Kumakuma (Brachyplathystoma filamentosum) skin using the liming method

Gelatin extraction from Kumakuma (Brachyplathystoma filamentosum) skin using the liming method

Influence of Freezing–Thawing Cycle on Water Dynamics of Turbot Flesh Assessed by Low-Field Nuclear Magnetic Resonance and Magnetic Resonance Imaging

Expression of the Laccase Gene from a White Rot Fungus in Pichia pastoris Can Enhance the Resistance of This Yeast to H 2 O 2 -Mediated Oxidative Stress by Stimulating the Glutathione-Based Antioxidative System

Contact Info

Product

Resources

About

Expression of the Laccase Gene from a White Rot Fungus in Pichia pastoris Can Enhance the Resistance of This Yeast to H ₂ O ₂ -Mediated Oxidative Stress by Stimulating the Glutathione-Based Antioxidative System