The Effect of Polyphenol Extract from Rosa Roxburghii Fruit on Plasma Metabolome and Gut Microbiota in Type 2 Diabetic Mice

Abstract

Rosa roxburghii fruit is an underutilized functional food abundant in polyphenols. Polyphenols have been proved to have antidiabetic effects. This study investigates the effects of Rosa roxburghii fruit polyphenols extract (RPE) on plasma metabolites and gut microbiota composition in streptozotocin (STZ)- and high-fat diet- induced type 2 diabetes using metabolomics and 16S rRNA gene sequencing. The induced diabetic mice were fed with 400 mg/kg body weight RPE for 8 weeks. RPE demonstrated hypoglycemic, hypolipidemic, and anti-inflammatory effects. Colonic oxidative stress biomarkers were also lowered by RPE. Besides, RPE decreased plasma ceramides and tyrosine levels and increased carnitine and phosphatidylinositols levels, indicating improved insulin resistance, lipid metabolism, and immune response. Furthermore, RPE decreased abundances of Lachnospiraceae and Rikenellaceae and increased abundances of Erysipelotrichaceae and Faecalibaculum. Metabolic function prediction of the gut microbiota by PICRUSt demonstrated that RPE downregulated the phosphotransferase system. Taken together, these findings demonstrated that RPE has the potential to prevent type 2 diabetes by regulating the plasma metabolites and gut microbes.

Effect of free and bound polyphenols from Rosa roxburghii Tratt distiller's grains on moderating fecal microbiota

“…( Jin et al, 2021 ). Baicalin was a natural flavonoid glycoside that attenuated intestinal oxidative stress by ameliorating the peroxidation of free radicals or enhancing the activity of antioxidant enzymes ( Wang et al, 2022 ). Notably, catechin and gallic acid, the most abundant flavonoids and phenolic acids in R. roxburghii DGs polyphenols, also made a major contribution to the phenolic antioxidant activity ( Fig.…”

“…Hence, it becomes essential to control gut microbiota homeostasis in order to maintain host health. Currently, sufficient evidences showed that diet and food components such as polyphenols can alter the gut microbial composition and promote intestinal health ( Luo et al, 2019 , Su et al, 2022 , Wang et al, 2022 , Wang et al, 2022 ).…”

Effect and Correlation of Rosa roxburghii Tratt Juice Fermented by Lactobacillus paracasei SR10-1 on Oxidative Stress and Gut Microflora Dysbiosis in Streptozotocin (STZ)-Induced Type 2 Diabetes Mellitus Mice

“…RRT is rich in bioactive substances, such as vitamin C, polysaccharides, polyphenols, and SOD, often used in the research and development of functional foods [13]. Polysaccharides [17] and polyphenols [22] in RRT have been proven to have hypoglycemic effects. The previous research of our team found that RRT fruit vinegar had an ameliorating effect on dyslipidemia and other phenomena in obese mice [25].…”

The mitigative effect of isorhamnetin against type 2 diabetes via gut microbiota regulation in mice

“…Polydipsia, polyphagia, polyuria, and emaciation are considered typical symptoms of T2DM and their presence is among the diagnostic criteria ( 16 ). In mice, a fasting blood glucose ≥11.1 mmol/L is the diagnostic criterion for T2DM ( 22 ). T2DM can alter glycolipid metabolism in patients ( 23 ).…”