The effect of polystyrene microplastic and biosolid application on the toxicity and bioaccumulation of cadmium for Enchytraeus crypticus

Abstract

Plastics smaller than 5 mm that end up in a soil environment are known as microplastics (MPs). Microplastics have become a common contaminant in agricultural areas in addition to metals. However, the effect of cadmium (Cd) on soil organisms has not been clearly defined in the presence of MPs. In addition to MPs, biosolid application as a soil amendment in agricultural lands is also leading to shifts in soil conditions, such as the concentrations of nutrients and organic matter. Therefore, the aim of this study is to investigate the toxicity and bioaccumulation of Cd for Enchytraeus crypticus in the presence of polystyrene (PS)-MPs and biosolids to provide insight into their possible interactions. The lethal toxic concentration (LC50) for Cd was higher than 650 mg Cd/kg dry soil for all conditions. The presence of PS-MPs increased the toxicity of Cd for which EC50 was 102 and 38 mg Cd/kg dry soil without and with Cd, respectively, which may be the result of an increased exposure rate through adsorption of Cd on PS-MPs. On the contrary, the presence of biosolids decreased the toxicity of Cd where EC50 was 193 and 473 mg Cd/kg dry soil for the sets applied with 0.6 and 0.9 g biosolids, respectively. Coexistence of biosolids and PS-MPs decreased the reproduction toxicity of Cd, which is similar to the biosolid effect (EC50 is 305 mg Cd/kg dry soil). Bioaccumulation of Cd only positively correlated with its initial concentration in soil and was not affected by the presence of PS-MPs or biosolids.