The effect of pretreatment using hydrochloric acid on the characteristics of buffalo hide gelatin

Mulyani, Sri; Setyabudi, F. M. C. S.; Pranoto, Yudi; Santoso, Umar

doi:10.14710/jitaa.42.1.14-22

Cited by 13 publications

(9 citation statements)

References 22 publications

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“…The pretreated hides were soaked in distilled water (1:4 w/v) at 65°C (Memmert WNB7-45 type water bath) for 5 h, followed by further soaking at 70°C for 5 h in preparation for the second extraction step. The extracted gelatin was sieved (< 200 mesh) and dried in a cabinet drier at 50-55°C for 48 h until the moisture less than 12% ( Mulyani et al , 2017 ), and the yield was calculated using the following formula ( Kim et al , 2012 ; Ktari et al , 2014 ):…”

Section: Methodsmentioning

confidence: 99%

Physicochemical Properties of Gelatin Extracted from Buffalo Hide Pretreated with Different Acids

Mulyani¹,

Setyabudi²,

Pranoto³

et al. 2017

Korean Journal for Food Science of Animal Resources

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The acid pretreatment of collagen molecules disrupts their crosslinks and assists in the release of acid-soluble proteins, fats, and other components. Generally, to achieve optimum extraction efficiency, strong acids may be used at a lower acid concentration compared to weak acids. This study aimed to determine the yield and physicochemical properties of gelatins extracted from buffalo hides pretreated with different acids. Hides were extracted with hydrochloric, citric, and acetic acids at concentrations of 0.3, 0.6, 0.9, 1.2, and 1.5 M. A completely randomized design and the least significant difference test were used in the experimental design, and all measurements were performed in triplicate. The highest yield (29.17%) was obtained from pretreatment with 0.9 M HCl. The gel strength did not differ significantly (p>0.05) according to acid type (280.26-259.62 g Bloom), and the highest viscosity was obtained from the 0.6 M citric acid pretreatment. All the gelatins contained α- and β-chain components and several degraded peptides (24-66 kDa). The color and Fourier-transform infrared spectrum of the gelatin extracted using 0.9 M HCl were similar to those of commercial bovine skin gelatin. In general, the physicochemical properties of the gelatin complied with the industry standard set by the Gelatin Manufacturers Institute of America, revealing that buffalo hide could serve as a potential alternative source of gelatin.

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Section: Methodsmentioning

confidence: 99%

Physicochemical Properties of Gelatin Extracted from Buffalo Hide Pretreated with Different Acids

Mulyani¹,

Setyabudi²,

Pranoto³

et al. 2017

Korean Journal for Food Science of Animal Resources

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“…Metode yang digunakan mengacu pada penelitian Mulyani et al (2017a), yaitu kulit kerbau dicuci menggunakan air mengalir untuk menghilangkan larutan garam yang merupakan pengawet pada kulit. Kemudian kulit direndam dalam air kapur 2% (b/v) untuk menghilangkan rambut pada kulit.…”

Section: Metode Penelitian Penyiapan Kulit Kerbauunclassified

“…Identifikasi karakteristik kolagen dari kulit kerbau meliputi nilai rendemen yang sesuai dengan penelitian Mulyani et al (2017b) dan kadar air yang sesuai panduan AOAC (2005). Uji berat molekul menggunakan SDS-PAGE dengan metode Laemmli (1970) dan uji gugus fungsional dengan menggunakan FTIR yang mengacu pada penelitian Mulyani et al (2017a).…”

Section: Pengujianunclassified

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Ekstraksi kolagen dari kulit kerbau menggunakan asam asetat

Mulyani

Hintono

Adefatma

et al. 2021

MKKP

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Buffalo hide is potential as an alternative raw material for making halal collagen to substitute those made from cow and porcine. However, the complexity of buffalo hide is higher than cattle hide and pigskin. Acetic acid is a weak acid that is able to extract collagen properly. The study aimed to determine the effect and the interaction of various concentrations of acetic acid and extraction time on collagen quality, including yield, water content, and collagen's characteristics based on molecular weight and functional groups. The main research material was 3 (three) sides of male buffalo (swamp buffalo) skin aged 3-4 years. This study was an experimental research with factorial completely randomized design, which consisted of the factor of acetic acid concentrations (3%, 5%, 7%) and the factor of extraction times (48 hours and 72 hours). All treatments were replicated four times. The optimum result of buffalo hide collagen extraction was using a concentration of 1 M acetic acid with 72 hours extraction time that produced collagen with the yield of 1.42±0.27%, based on the concentration factor, and the yield of 1.70 ± 0.35%, based on the extraction time factor, 4.19±0.14% water content, and it was identified that collagen has not yet been degraded into gelatin based on molecular weight and functional groups.

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“…The average level of gelatin protein in sheepskin type A in this study is higher when compared with the results of research Zilhadia et al, (2018) which produces an average level of goatskin protein that is 86.58%, this is caused by differences in raw materials, methods pretreatment and duration of soaking in HCl solution. Long immersion time and pretreatment material concentration cause collagen fibres to shrink and break into irregular structures so that they undergo a perfect dissolution process during the process of hydrolysis of skin collagen (Mulyani et al, 2017).…”

Section: Protein Contentmentioning

confidence: 99%

Comparative Quality of Proteins and Morphological Structures of Gelatin From Sheepskin With Acid and Alkaline Treatment

Hasdar¹,

Randi²

2020

FST

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Sheepskin is one alternative to raw materials for producing gelatin. This study aims to compare the quality of gelatin from sheepskin based on different types of pretreatment solutions used, namely HCl 1,5% solution and NaOH 1,5% solution. Data analysis used t-test to compare two treatment methods. Yield and protein gelatin there is a difference where the treatment of HCl 1,5% solution is higher than the treatment of NaOH 1,5% solution. The brighter visual quality of gelatin colour was produced by HCl 1,5% treatment compared to NaOH 1,5% and commercial gelatin treatments. The results of the microstructure using Scanning Electron Microscopy (SEM) showed that there were still lots of clumps of protein and cavities in the gelatin NaOH 1,5% and commercial gelatin treatments. Gelatin with HCl 1,5% treatment showed the best results.

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The effect of pretreatment using hydrochloric acid on the characteristics of buffalo hide gelatin

Cited by 13 publications

References 22 publications

Physicochemical Properties of Gelatin Extracted from Buffalo Hide Pretreated with Different Acids

Physicochemical Properties of Gelatin Extracted from Buffalo Hide Pretreated with Different Acids

Ekstraksi kolagen dari kulit kerbau menggunakan asam asetat

Comparative Quality of Proteins and Morphological Structures of Gelatin From Sheepskin With Acid and Alkaline Treatment

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