This cross-sectional comparative survey aimed to determine the level differences of professional nursing values in sophomore and senior nursing students using a demographic form and the Nurses Professional Values Scale-3. The mean score for nursing professional values was significantly higher ( p < .001) among senior students (111.02, SD = 15.12) than sophomore students (99.46, SD = 12.56). However, professional values in the professional dimension require improvement. To ensure that future nurses integrate these values into professional practice, nurse educators must teach professional values by adopting innovative approaches KEY WORDS Ethical Values -Nursing Students in India -Professional Values -Sophomore Students