The effect of proline-rich polypeptide on reduction of methemoglobin and formation of superoxide by the b558III cytochrome and suprol metalloproteins in vitro

Simonyan, G. M.; Simonyan, R. M.; Aghamyan, G. R.; Simonyan, M. A.; Galoyan, A. A.

doi:10.1134/s1819712407020080

Cited by 1 publication

(4 citation statements)

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“…The experiments carried out by Dr. K. Mohanakumar (India) have indicated that the Galarmin-lean solution (nanomol range) really is an effective scavenger for HO • radicals (unpublished data). The data obtained confirm the results obtained earlier by Galoyan and coworkers on Galarmin effect in vivo [6].…”

Section: Introductionsupporting

confidence: 96%

“…It testifies that Galarmin possesses sound redox properties. Simultaneously, the oxidation specific peak in DPV voltammograms within the mentioned potential range predicts also existence of Galarmin antiradical activity [6], i.e. Galarmin is able to capture free radicals which attack vital cell components inducing pathologies of oxidative stress.…”

Section: Resultsmentioning

confidence: 98%

“…The electrochemical analysis system was monitored with the use of standard Fe(CN) 6 -3 solution, for which a linear calibration plot with a correlation coefficient of 0.9995 was obtained previously.…”

Section: Electrochemical Measurementsmentioning

confidence: 99%

“…However, increase in the Galarmin concentration resulted in a decrease in the superoxide-producing activity of the b558 III Cyt. It was also demonstrated that the Galarmin inhibited superoxide formation in response to the action of the suprol lipoprotein in blood serum [6].…”

Section: Introductionmentioning

confidence: 97%

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Antioxidant and Electron Donating Function of Hypothalamic Polypeptides: Galarmin and Gx-NH2

et al. 2010

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Chemical mechanisms of antioxidant and electron donating function of the hypothalamic proline-rich polypeptides have been clarified on the molecular level. The antioxidant-chelating property of Galarmin and Gx-NH(2) was established by their capability to inhibit copper(II) dichloride catalyzed H(2)O(2) decomposition, thus preventing formation of HO(*) and HOO(*) radicals. The antiradical activity of Galarmin and Gx-NH(2) was determined by their ability to react with 2,2-diphenyl-1-picrylhydrazyl radical applying differential pulse voltammetry and UV-Vis spectrophotometry methods. Galarmin manifest antiradical activity towards 2,2-diphenyl-1-picrylhydrazyl radical, depending on the existence of phenolic OH group in tyrosine residue at the end of the molecule. The presence of antiradical activity and reduction properties of Galarmin are confirmed by the existence of an oxidation specific peak in voltammograms made by differential pulse voltammetry at E ( composite function) = 0.795 V vs. Ag/Ag(+) aq.

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