The Effect of Protein Fractions from Rabbit Uterine Fluids on Embryo Growth and Uptake of Nucleic Acid and Protein Precursors

Macromolecular Components of the Luminal Fluid From the Bovine Uterus

“…It appears, however, that the rabbit is unique in this respect and al¬ though blastokinin-like materials have been reported in other species (e.g. mink: Daniel, 1968; pig and dog: Daniel & Krishnan, 1969; northern fur seal: Daniel, 1972;man: Shirai et al, 1972), they are present in low concentrations and have not been well characterized.…”

“…Since blastulation occurs at about Day 8 in the cow, it is at this time that one might expect to find a protein analogous to blastokinin which has been reported to influence blastocyst formation in the rabbit (Krishnan & Daniel, 1967;El-Banna & Daniel, 1972 Squire, Bazer & Murray, 1972) at a similar stage of blastocyst development and that at least one of these is a cathode-migrating protein on disc electrophoresis. In the pig, the appear¬ ance of these uterine-specific proteins has been shown to be progesteronedependent (Knight, Bazer & Wallace, 1973 (Pope, Gupta & Munro, 1969).…”

Development retardation in cultured preimplantation rabbit embryos

“…In all these studies, treatment of uterine flushings by dialysis, lyophilization, fractionation, storage, or adjustment to a given protein concentration was involved. In most cases, best development occurred in unfractionated uterine flushings (El-Banna & Daniel, 1972b;Maurer & Beier, 1976;Beier et ai, 1983 Guilbert-Blanchette & Lambert (1978) found almost the same percentage of rabbit morulae developed into blastocysts in flushings derived from 'oestrous' or pseudopregnant rabbits of different times after hCG.…”

“…In earlier studies by El-Banna & Daniel (1972b) there was marked variability in the response of the embryos to supplementation with uterine protein fractions. Variation in embryo size and thymidine incorporation were also found in the present study.…”

Response of Mouse Embryos and Uterus to Tumour Extract Treatment

“…Mammalian embryos, particularly those of pre-implantation stages, are ex tremely sensitive to most types of treatments [6], This might explain the finding that even normal FBE adversely affects embryogenesis. But the effects of tumour extract are far more extensive than those of FBE and are statistically significant.…”