The effect of quercetin on the electrical properties of model lipid membranes and human glioblastoma cells

Kruszewski, Marcin Andrzej; Kusaczuk, Magdalena; Kotyńska, Joanna; Gál, Miroslav; Krętowski, Rafał; Cechowska-Pasko, Marzanna; Naumowicz, Monika

doi:10.1016/j.bioelechem.2018.07.010

Cited by 21 publications

(22 citation statements)

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“…It is noteworthy that theoretical and experimental results are in line with each other within the analyzed pH range (between pH 2 and pH 10). As such, below and above this range, cells might undergo cell death, which is typical for normal and cancer cells [ 16 , 56 ]. LN-229 cells treated with either CA or FA showed an increase in positive charge at low pH and a decrease in negative charge at high pH in comparison to untreated cells.…”

Section: Discussionmentioning

confidence: 99%

“…Briefly, cells were grown in DMEM containing 10% fetal bovine serum, 4.5 mg/cm 3 glucose, 100 μg/cm 3 streptomycin, 100 U/cm 3 penicillin, and 2 mmol/dm 3 L-glutamine and kept in an incubator (humidified atmosphere, 5% CO 2, 37 °C). Cells reaching confluence were seeded into 96-well plates (Nunclone) in a density of 1000 cells/well and growth medium was substituted with DMEM containing various concentrations of CA (0.25–8.00 mmol/dm 3 ) or FA (0.0625–8.00 mmol/dm 3 ) and further incubated for 24 h and 48 h. After incubation, cell viability was estimated using the metabolic activity-assessing tetrazolium test (MTT assay) performed according to the method of Carmichael et al [ 62 ] described in detail in our previous work [ 16 ].…”

Section: Methodsmentioning

confidence: 99%

“…One of the techniques that might be used to estimate zeta potential is the electrophoretic light scattering (ELS) technique based on dynamic light scattering (DLS), in which the shift in the frequency or in the oscillation phase of the laser beam depends on the mobility of particles/cells in an alternating electric field [ 14 ]. In this respect, in our previous works, we have already applied the ELS technique to study human glioblastoma cells treated with either silica nanoparticles [ 14 , 15 ] or phenolic compounds such as quercitin [ 16 ] and p-coumaric acid [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

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Monitoring of the Surface Charge Density Changes of Human Glioblastoma Cell Membranes upon Cinnamic and Ferulic Acids Treatment

Naumowicz

Kusaczuk

Zając

et al. 2020

IJMS

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Cinnamic acid (CA) and ferulic acid (FA) are naturally occurring phenolic acids claimed to exert beneficial effects against disorders related to oxidative stress, including cancer. One such malignancy that still remains a therapeutic challenge mainly due to its heterogeneity and inaccessibility to therapeutic agents is Glioblastoma multiforme (GBM). Here, the influence of CA and FA on the surface charge density of human GBM cell line LN-229 was studied using the electrophoretic light scattering technique. Also, the cytotoxicity of both phenolic acids was determined by metabolic activity-assessing tetrazolium test (MTT) analysis after exposure to CA and FA for 24 h and 48 h. Results showed that both compounds reduced cell viability of LN-229 cells, with more pronounced effect evoked by CA as reflected in IC50 values. Further analyses demonstrated that, after treatment with both phenolic acids, the negative charge of membranes decreased at high pH values and the positive charge of the membranes increased at low pH values compared to the data obtained for untreated cells. Afterward, a four-equilibrium model was applied to estimate the total surface concentrations of both acidic and basic functional groups and their association constants with solution ions in order to calculate theoretical values of membrane surface charge densities. Then, the theoretical data were compared to the experimental data in order to verify the mathematical model. As such, our results indicate that application of electrochemical methods to determine specific drug–membrane interactions might be crucial for predicting their pharmacological activity and bioavailability.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Monitoring of the Surface Charge Density Changes of Human Glioblastoma Cell Membranes upon Cinnamic and Ferulic Acids Treatment

Naumowicz

Kusaczuk

Zając

et al. 2020

IJMS

Self Cite

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“…Briefly, cell lines were grown in DMEM containing 10% fetal bovine serum, 4.5 mg/cm 3 glucose, 100 μg/cm 3 streptomycin, 100 U/cm 3 penicillin, and 2 mmol/dm 3 L-glutamine, and kept in an incubator (humidified atmosphere, 5% CO 2, 37 °C). Confluent cells were supplemented with p-CoA (0–10 mmol/dm 3 ) and incubated for 24 and 48 h. Then, all cell lines were subjected to MTT analysis performed according to the method of [50], described in detail in our previous work [51]. Based on the MTT results, two concentrations of p-CoA (5 and 8 mmol/dm 3 ) were chosen to proceed for further Z-potential examinations.…”

Section: Methodsmentioning

confidence: 99%

The Modulating Effect of p-Coumaric Acid on The Surface Charge Density of Human Glioblastoma Cell Membranes

Kruszewski

Kotyńska

Kusaczuk

et al. 2019

IJMS

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p-Coumaric acid (p-CoA), a phenolic acid belonging to the hydroxycinnamic acids family, is a compound with tentative anticancer potential. Microelectrophoretic mobility measurements conducted at various pH values of electrolyte solution were applied to study p-CoA effects on electrical properties of human glioblastoma cell membranes. The obtained results demonstrated that after the p-CoA treatment, the surface charge density of cancer cells changed in alkaline pH solutions, while no noticeable changes were observed in cell membranes incubated with p-CoA compared to control at acidic pH solutions. A four-equilibrium model was used to describe the phenomena occurring on the cell membrane surface. The total surface concentrations of both acidic and basic functional groups and their association constants with solution ions were calculated and used to define theoretical curves of membrane surface charge density versus pH. The resulting theoretical curves and the experimental data were compared to verify the reliability and validity of the adopted model. The deviation of both kinds of data obtained at a higher pH may be caused by disregarding interactions between the functional groups of cancer cells. Processes occurring in the cell membranes after their incubation with p-CoA can lead to disorders of existing equilibria, which result in changes in values of the parameters describing these equilibria.

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“…In basal cell carcinomas, MTSS1 has also been identified as a sonic hedgehog responsive gene, potentiating Gli-dependent expression [128]. Additionally, MTSS1 regulates the EGFR signaling pathway [129]. MTSS1 DNA methylation and transcriptional silencing has been reported in bladder cancer cells, with detection of a promoter activity region 276 bp upstream of the metastasis suppressor protein 1 (MTSS1) gene within a CpG island [130].…”

Section: Epigenetics In Glioblastomamentioning

confidence: 99%

Advances in Targeting the Epidermal Growth Factor Receptor Pathway by Synthetic Products and Its Regulation by Epigenetic Modulators as a Therapy for Glioblastoma

Abbas

Kausar

Wang

et al. 2019

Cells

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Glioma is the most common primary tumor of the nervous system, and approximately 50% of patients exhibit the most aggressive form of the cancer, glioblastoma. The biological function of epidermal growth factor receptor (EGFR) in tumorigenesis and progression has been established in various types of cancers, since it is overexpressed, mutated, or dysregulated. Its overexpression has been shown to be associated with enhanced metastatic potential in glioblastoma, with EGFR at the top of a downstream signaling cascade that controls basic functional properties of glioblastoma cells such as survival, cell proliferation, and migration. Thus, EGFR is considered as an important therapeutic target in glioblastoma. Many anti-EGFR therapies have been investigated both in vivo and in vitro, making their way to clinical studies. However, in clinical trials, the potential efficacy of anti-EGFR therapies is low, primarily because of chemoresistance. Currently, a range of epigenetic drugs including histone deacetylase (HDAC) inhibitors, DNA methylation and histone inhibitors, microRNA, and different types of EGFR inhibitor molecules are being actively investigated in glioblastoma patients as therapeutic strategies. Here, we describe recent knowledge on the signaling pathways mediated by EGFR/EGFR variant III (EGFRvIII) with regard to current therapeutic strategies to target EGFR/EGFRvIII amplified glioblastoma.

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The effect of quercetin on the electrical properties of model lipid membranes and human glioblastoma cells

Cited by 21 publications

References 50 publications

Monitoring of the Surface Charge Density Changes of Human Glioblastoma Cell Membranes upon Cinnamic and Ferulic Acids Treatment

Monitoring of the Surface Charge Density Changes of Human Glioblastoma Cell Membranes upon Cinnamic and Ferulic Acids Treatment

The Modulating Effect of p-Coumaric Acid on The Surface Charge Density of Human Glioblastoma Cell Membranes

Advances in Targeting the Epidermal Growth Factor Receptor Pathway by Synthetic Products and Its Regulation by Epigenetic Modulators as a Therapy for Glioblastoma

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