The Effect of Rapeseed Oil Uptake on the Production of Erythromycin and Triketide Lactone by <i>Saccharopolyspora erythraea</i>

Mirjalili, Noushin; Zormpaidis, Vassilios; Leadlay, Peter F.; Ison, A. P.

doi:10.1021/bp990099h

Cited by 13 publications

(5 citation statements)

References 52 publications

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“…Because biomass is one the factors most affecting the fouling of membranes in the microfiltration process [9], a lower biomass production can cause a decrease in the cost of down-stream processing. Rapeseed oil had a significant effect on the production of erythromycin, as indicated recently [9,22], but its beneficial effect was not as high as that of melon seed oil. Growth of S. erythraea NUR001 was higher in rapeseed oil-containing medium and the utilization of this oil was the greatest, when compared with that of other oils, thus giving more erythromycin production.…”

Section: Discussionmentioning

confidence: 89%

“…Oils can serve as sources of precursors for the biosynthesis of 6-erythronolide B [7,13,14]. Recently, the efficiency of rapeseed oil in the production of erythromycin by S. erythraea was shown [9,22]. However, few studies have been directed to evaluate the comparative effect of different oils on the production of erythromycin and the correlation of growth and product [11].…”

Section: Introductionmentioning

confidence: 99%

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Enhancing of erythromycin production by Saccharopolyspora erythraea with common and uncommon oils

Hamedi

Malekzadeh

Saghafi-nia³

2004

J IND MICROBIOL BIOTECHNOL

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The enhancing effect of various concentrations of 18 oils and a silicon antifoam agent on erythromycin production by Saccharopolyspora erythraea was evaluated in a complex medium containing soybean flour and dextrin as the main substrates. The oils used consisted of sunflower, pistachio, cottonseed, melon seed, water melon seed, lard, corn, olive, soybean, hazelnut, rapeseed, sesame, shark, safflower, coconut, walnut, black cherry kernel and grape seed oils. The biomass, erythromycin, dextrin and oil concentrations and the pH value were measured. Also, the kinds and frequencies of fatty acids in the oils were determined. The productivity of erythromycin in the oil-containing media was higher than that of the control medium. However, oil was not suitable as a main carbon source for erythromycin production by S. erythraea. The highest titer of erythromycin was produced in medium containing 55 g/l black cherry kernel oil (4.5 g/l). The titers of erythromycin in the other media were also recorded, with this result: black cherry kernel > water melon seed > melon seed > walnut > rapeseed > soybean > (corn = sesame) > (olive = pistachio = lard = sunflower) > (hazelnut = cotton seed) > grape seed > (shark = safflower = coconut). In media containing various oils, the hyphae of S. erythraea were longer and remained in a vegetative form after 8 days, while in the control medium, spores were formed and hyphae were lysed.

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Section: Discussionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Enhancing of erythromycin production by Saccharopolyspora erythraea with common and uncommon oils

Hamedi

Malekzadeh

Saghafi-nia³

2004

J IND MICROBIOL BIOTECHNOL

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“…Oils are attractive carbon substrates, because the oxidation of fatty acids not only can serve as a source of energy for the cells, but the formation of acetyl-CoA as a degradation product can also provide precursors for epothilone biosynthesis. The addition of oils to the fermentation of Saccharopolyspora erythraea, Streptomyces fradiae, and Streptomyces hygroscopicus has been shown to enhance the production of polyketide molecules, such as erythromycin, tylosin, and the immunoregulant L-683590, respectively (Choi et al, 1998;Junker et al, 1998;Mirjalili et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

Optimizing the heterologous production of epothilone D in Myxococcus xanthus

Lau¹,

Frykman²,

Regentin³

et al. 2002

Biotech & Bioengineering

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The heterologous production of epothilone D in Myxococcus xanthus was improved by 140-fold from an initial titer of 0.16 mg/L with the incorporation of an adsorber resin, the identification of a suitable carbon source, and the implementation of a fed-batch process. To reduce the degradation of epothilone D in the basal medium, XAD-16 (20 g/L) was added to stabilize the secreted product. This greatly facilitated its recovery and enhanced the yield by three-fold. The potential of using oils as a carbon source for cell growth and product formation was also evaluated. From a screen of various oils, methyl oleate was shown to have the greatest impact. At the optimal concentration of 7 mL/L in a batch process, the maximum cell density was increased from 0.4 g dry cell weight (DCW)/L to 2 g DCW/L. Product yield, however, depended on the presence of trace elements in the production medium. With an exogenous supplement of trace metals to the basal medium, the peak epothilone D titer was enhanced eight-fold. This finding demonstrates the significant role of metal ions in cell metabolism and in epothilone biosynthesis. To further increase the product yield, a continuous fed-batch process was used to promote a higher cell density and to maintain an extended production period. The optimized fed-batch cultures consistently yielded a cell density of 7 g DCW/L and an average production titer of 23 mg/L.

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“…Both have previously been used in fermentation studies within our laboratory (Heydarian, 1998;Mirjalili et al, 1999;Sarra et al, 1996). Spore stocks were initially prepared from surface cultures of S. erythraea, the medium used contained 2 g L −1 glucose, 1 g L −1 sucrose, 5 g L −1 soy peptone, 2.5 g L −1 yeast extract, 20 g L −1 agar (Technical No.…”

Section: Fermentation Media and Operating Conditionsmentioning

confidence: 99%

Studies on the interaction of fermentation and microfiltration operations: Erythromycin recovery fromSaccharopolyspora erythraea fermentation broths

Davies

Baganz

Ison

et al. 2000

Biotechnol. Bioeng.

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Changes in fermentation media not only affect the performance of the fermentation itself (with regard to the kinetics of biomass and product formation and the yields obtained) but also the initial product‐recovery operations downstream of the fermentor. In this work, microfiltration experiments to remove Saccharopolyspora erythraea biomass from fermentation broth and to recover erythromycin were carried out using two fundamentally different media; a soluble complex medium (SCM) and an oil‐based process medium (OBM). Small‐scale batch fermentations of 14‐L working volume were carried out in triplicate using both media. Broth samples were taken from each fermentation at regular intervals from the end of the exponential‐growth phase onwards. These were then processed using a Minitan II (acrylic), tangential crossflow‐filtration module, fitted with a single 60 cm2 Durapore hydrophilic 0.2 μm membrane, operated in concentration mode. The OBM fermentations produced higher titers of erythromycin but required longer fermentation times due to increased lag phases and slower maximum‐growth rates. The OBM also increased the loading on the membrane; at maximum product titers residual oil concentrations of 3 g · L−1, antifoam concentrations of 2 g · L−1 and flour concentrations estimated at approximately 10 g/L−1 were typical. It was found that both the permeate flux and erythromycin transmission were affected by the choice of medium. The OBM had significantly lower values for both parameters (12.8 Lm−2 h−1 and 89.6% respectively) than the SCM (35.9 Lm−2 h−1 and 96.7% respectively) when the fermentations were harvested at maximum erythromycin titers. Transmission of erythromycin stayed approximately constant as a function of fermentation time for both media, however, for the OBM the permeate flux decreased with time which correlated with an increase in broth viscosity. The relatively poor microfiltration performance of the OBM medium was, however, offset by the higher titers of erythromycin that were achieved during the fermentation. The filtration characteristics of the SCM broth did not show any correlation with either broth viscosity or fermentation time. Image‐analysis data suggested that there was a correlation between hyphal morphology (main hyphal length) and permeate flux (no such correlation was found for the OBM broth). Moreover, it has been shown for the OBM broth that the residual flour had a profound effect on the microfiltration characteristics. The influence of the residual flour was greater than that imposed by the morphology and concentration of the biomass. The understanding of the factors governing the interaction of the fermentation and microfiltration operations obtained in this work provides a first step towards optimization of the overall process sequence. © 2000 John Wiley & Sons, Inc. Biotechnol Bioeng 69: 429–439, 2000.

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The Effect of Rapeseed Oil Uptake on the Production of Erythromycin and Triketide Lactone by Saccharopolyspora erythraea

Cited by 13 publications

References 52 publications

Enhancing of erythromycin production by Saccharopolyspora erythraea with common and uncommon oils

Enhancing of erythromycin production by Saccharopolyspora erythraea with common and uncommon oils

Optimizing the heterologous production of epothilone D in Myxococcus xanthus

Studies on the interaction of fermentation and microfiltration operations: Erythromycin recovery fromSaccharopolyspora erythraea fermentation broths

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