The effect of refined functional carbohydrates from enzymatically hydrolyzed yeast on the transmission of environmental Salmonella Senftenberg among broilers and proliferation in broiler housing

Abstract

Hatching eggs collected from resident broiler breeders at 48 wk of age were used to produce male and female chicks that were assigned sex separately to 96 new litter pens and fed either a 0 or 50 g/MT RFC (refined functional carbohydrate feed additive derived from yeast) diet. There were 24 replicate pens of 12 broilers each per diet per sex. Feed intake and BW were determined at 14, 28, and 42 d of age. Litter was sampled by pen using sterile socks at 35 d and tested for Salmonella spp. using an enzyme linked fluorescence assay method. Salmonella spp. was isolated from 7 of 48 control-fed broiler pens but no RFC-fed pens (P ≤ 0.05). Thereafter, 48 males and 48 females were selected based on litter Salmonella presence and RFC treatment. The cecas of these broilers were aseptically excised after feed withdrawal and lairage and tested for presence of Salmonella spp. There were 18 of the 48 control-fed broilers confirmed positive from litter-positive pens but none from litter-negative pens fed RFC. The serovar of litter and cecal Salmonella isolates was Salmonella enterica subsp. enterica serovar Senftenberg (S. Senftenberg). Female broilers that were fed RFC exhibited greater BW at 28 d (P ≤ 0.05) and 42 d (P ≤ 0.05) while RFC-fed males exhibited improved feed efficiency during the 15-28 d period (P = 0.06). These data demonstrated that dietary RFC reduced the prevalence of Salmonella in the litter and ceca of broilers when fed continuously while not being detrimental to broiler live performance.

The effect of a selected yeast fraction on the prevention of pullorum disease and fowl typhoid in commercial breeder chickens

“…It is inferred that supplementation of SYF in the diet helps to improve the feed conversion before the breeding stage of pullets, and the decrease of BW caused by Salmonella infection in chicks was significantly eased, which is consistent with previous research reports listed here. Refined functional carbohydrate feed additive derived from yeast supplemented in the diet increased the weight gain, daily gain and feed intake (Walker et al, 2018 ). As the dosage increased, there is an uptrend.…”

Saccharomyces cerevisiaeas a probiotic feed additive to non and pseudo‐ruminant feeding: a review

“…The enzymatic hydrolysis of yeasts produces cell‐free culture supernatant that show activity against Gram‐negative bacteria. Previous studies have shown that refined functional carbohydrates can agglutinate as well as inhibit adherence of several species of Salmonella to the intestinal epithelium inhibiting them from colonizing the digestive tract (Walker et al , ). Singh et al () also showed that refined functional carbohydrates have effects on cytokine gene expression, including the genes encoding for pro‐inflammatory cytokines and T helper (T1and T2), which enhances the immune function of sexually mature hens.…”

Research Note: Repetitive element–based polymerase chain reaction genotyping improves efficiency of Salmonella surveillance in a model broiler production system

“…The following samples were collected from BF1 as described by Walker et al. (2018) : feed samples from each experimental diet at the feed mill, chick paper, and eggshells in the hatchery at day of hatch, a pre-enriched sock applied to litter floor pens at 15 D, and individual ceca at 44 D. Samples were suspended in 1% buffered peptone water ( BPW ) and maintained on ice until further enrichment and incubation occurred.…”

“…After initial enrichment for all samples, Salmonella spp. identification was achieved with an enzyme-linked fluorescence assay automated instrument (VIDAS 30 Multi-parametric Immunoassay Instrument, BioMérieux, Inc., Marcy-l’Étoile, France) and confirmed by culture with Rapid Salmonella Agar (Bio-Rad #3563961; Hercules, CA) and XLT-4 agar (Oxoid Product #CM1061) as described by Walker et al. (2018) .…”