The effect of Relenza treatment on the early immune response induced after influenza vaccination

The postvaccination antibody response to influenza virus proteins

“…In primed subjects, IgG is the most frequently detected class of serum antibody following vaccination (5)(6)(7)(8). Previously, we have examined the kinetics of the systemic and local humoral immune response (6)(7)(8)(9) and found that a rapid immune response occurs within a week of vaccination in the majority of subjects. This work was extended to examine the specificity of the early antibody response to the viral proteins of the three virus components in the vaccine using the radio-immunoblot.…”

Antibody Detection and Kinetics of Antibody Production during Early Stages of Immunization with Hepatitis B Virus Vaccine

“…The enzyme-linked immunospot assay is a well-established method for the study of the immune response and the secretion of antibodies from B lymphocytes in both natural infections and vaccine experiments (5,6,9,11,12), although the procedures are laborious and include time-consuming incubation steps. Further development based on this technology has revealed that specific antibodies can be detected after purified lymphocytes were placed in appropriately coated enzymelinked immunosorbent assay (ELISA) wells and kept for 1 to 2 h at 37°C to allow the spontaneous secretion of antibodies, referred to here as the PlasmAcute technology (9).…”

“…This response can be detected at about day 2 or 3 to day 16 or 17 following vaccination. Plasma immunoglobulin G (IgG) can usually be detected from day 12 onwards in this system (5,6).…”