The Effect of Seed Source and Media Composition on the Production of Canthin-6-ones in Cell Suspension Cultures ofBrucea javanica

Abstract

Posters mononetin (1)20.09 mm, and glycyrrhetinic acid 40 mm. In the present study, C. glabra suspension cultures produced mainly formononetin and not glycyrrhizin or glycyrrhetic acid. Fig. 1 shows the time course of growth and formononetin production in cell suspension cultures. The maximum cell mass of 10.03 g dry weight/I was attained 12 days after the transfer. The content of formononetin in cells began to increase at the late linear growth stage, and the maximum yield of 9.60mg formononetinlg dry weight was observed on day 15, after the cessation of cell growth. Once the maximum was reached the formononetin content declined in the stationary phase.