The effect of selected staining techniques on bull sperm morphometry

Banaszewska, Dorota; Andraszek, Katarzyna; Czubaszek, Magdalena; Biesiada–Drzazga, Barbara

doi:10.1016/j.anireprosci.2015.06.019

Cited by 26 publications

(26 citation statements)

References 60 publications

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“…Furthermore, the difference in measurement results can be due to the difference in the staining method applied that can alter the morphometry dimensions (Maree et al 2010). Staining applied in a particular species may not be applicable in other species (Banaszewska et al 2015).…”

Section: Resultsmentioning

confidence: 99%

Morphometry and abnormality evaluation of sex-sorted sperm of spotted buffalo (Tedong bonga)

2017

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Abstract. Kaiin EM, Gunawan M, Maulana T. 2017. Morphometry and abnormality evaluation of sex-sorted sperm of spotted buffalo (Tedong bonga). Nusantara Bioscience 9: 175-180. This research aimed to determine the morphometry and the abnormality of the spotted buffallo (Tedong bonga) sperm after the separation of X and Y-chromosome-bearing sperms. Semen was collected from the spotted buffalo bull by using an artificial vagina.The semen quality was evaluated macro-and microscopically. Semen that met the required standard were subjected to separation by using Bovine Serum Albumin (BSA) 5% and 10% column method. The quality of sex-sorted sperm was evaluated microscopically, including motility, concentration, viability, abnormality and intact plasma membrane. Smear preparation using Eosin-Nigrosin dyes was made for sperm morphometry and abnormality evaluation.The evaluation of sperm morphometry and abnormality was conducted on 200 sperm cells for each observation and repeated in triplicates of observation, by using Axiovision Imager Z microscope at objective and ocular magnifications 40x and 10x, respectively.The morphological parameters observed were including the length and the width of head sperm and the head area of sperm X or Y. The abnormality parameter consisted of primary and secondary abnormalities. The data were analyzed descriptively. The results showed that the head size of the Y sperm of spotted buffalo (27.16 µm 2 ) was smaller than that of the X sperm (29.86 µm 2 ) with the abnormality of the Y sperm (7.85%) was also lower than that of the X sperm (9.7%).

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Section: Resultsmentioning

confidence: 99%

Morphometry and abnormality evaluation of sex-sorted sperm of spotted buffalo (Tedong bonga)

2017

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“…Value equals to 1 indicates the perfect ellipse. The rugosity characterizes the amorphous cells, also being assigned values in scale from −1 to +1.This parameter can be positively correlated with head length, as well as being an indication of cell susceptibility to damage/rupture(Banaszewska, Andraszek, Czubaszek, & Biesiada-Drzazga, 2015;Leite, 2011;Sánchez, Bastir, & Roldan, 2013).…”

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confidence: 99%

Morphological and morphometric characterization of domestic cat epididymal sperm

Barbosa

Silva

Tabosa

et al. 2019

Reprod Domestic Animals

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Sperm morphometry is the tool that confers objectivity to the morphological evaluation by accurately measuring the dimensions of the gamete and its structures. Thus, the aim of the study was to perform a morphometric characterization of the domestic cat sperm. Therefore, sperm samples were collected from twenty pairs of epididymis in a TRIS extender at 37ºC. An aliquot of the sample was used to make a smear with Rose Bengal solution, and afterwards, the morphology and morphometry were analysed. In the morphology, were quantified the percentage of normal sperm cells, morphological changes of head, midpiece and tail. In morphometry, each normal sperm cell was measured for length, width, area and perimeter of head and midpiece, tail length and total length. The parameters ellipticity, elongation, regularity and rugosity were also determined. The percentage of normal sperm was 67.21%. Of the abnormalities, the curled/folded tail, followed by the curved midpiece, abnormal shaped head and detached head were the most quantified. The sperm head presented 5.56 ± 0.01 μm and 3.10 ± 0.01 μm of length and width, respectively. The head area was 16.94 ± 0.05 μm2, while the perimeter was 16.16 ± 0.03 μm. In the derived parameters, the values were as follows: ellipticity of 1.81 ± 0.00; elongation of 21.39 ± 0.12; regularity of 0.81 ± 0.00; and rugosity of 0.14 ± 0.00. The midpiece presented length and width of 7.96 ± 0.01 μm and 0.76 ± 0.01 μm, respectively. The mean length of the sperm tail was 45.12 ± 0.06 μm, and the total cell size was 58.67 ± 0.06 μm. Thus, it was concluded that the cat sperm is an elongated cell, with high rugosity and regularity. The spermatic tail represents more than ¾ of the total length of the cell and the midpiece exceeds the length of the head.

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“…All these steps can affect not only the repeatability of the experiments but also the reproducibility and the comparison of results among laboratories, which are necessary for the practical use of sperm morphometric analyses. These aspects have been studied by several authors,6465666768697071 and they have not yet been completely resolved.…”

Section: Assessment Of Sperm Morphometry I – Technological Aspectsmentioning

confidence: 99%

“…The choice of staining protocol is the issue on which most authors have conducted their studies63656668707172737980 since it influences background noise, sperm contrast, and the identification of different areas within the cell. To prevent these problems during morphometric sperm analysis, fluorescence-based methods in combination with a CASA-Morph system have been developed for a more precise measurement of the nucleus, acrosome, and sperm head, yielding promising results 6481.…”

Section: Assessment Of Sperm Morphometry I – Technological Aspectsmentioning

confidence: 99%

Current status and potential of morphometric sperm analysis

et al. 2016

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The spermatozoon is the most diverse cell type known and this diversity is considered to reflect differences in sperm function. How the diversity in sperm morphology arose during speciation and what role the different specializations play in sperm function, however, remain incompletely characterized. This work reviews the hypotheses proposed to explain sperm morphological evolution, with a focus on some aspects of sperm morphometric evaluation; the ability of morphometrics to predict sperm cryoresistance and male fertility is also discussed. For this, the evaluation of patterns of change of sperm head morphometry throughout a process, instead of the study of the morphometric characteristics of the sperm head at different stages, allows a better identification of the males with different sperm cryoconservation ability. These new approaches, together with more studies employing a greater number of individuals, are needed to obtain novel results concerning the role of sperm morphometry on sperm function. Future studies should aim at understanding the causes of sperm design diversity and the mechanisms that generate them, giving increased attention to other sperm structures besides the sperm head. The implementation of scientific and technological advances could benefit the simultaneous examination of sperm phenotype and sperm function, demonstrating that sperm morphometry could be a useful tool for sperm assessment.

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The effect of selected staining techniques on bull sperm morphometry

Cited by 26 publications

References 60 publications

Morphometry and abnormality evaluation of sex-sorted sperm of spotted buffalo (Tedong bonga)

Morphometry and abnormality evaluation of sex-sorted sperm of spotted buffalo (Tedong bonga)

Morphological and morphometric characterization of domestic cat epididymal sperm

Current status and potential of morphometric sperm analysis

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