The Effect of Self Assembling Peptides Versus Sodium Fluoride Varnish on Artificially Induced Enamel Lesions in Human Premolars, In Vitro Study. (Scanning Electron Microscope and Energy Dispersive X-ray Analysis)

Abstract

Background: Dental caries is a global health concern, with white spot lesions being a critical stage that can be reversed with preventive measures. Topical fluoride is commonly used, but researchers are exploring bioactive materials like self-assembling peptide P11-4 for biomimetic enamel remineralization.Aim: Assessment of biomimetic self assembling peptides P11-4 effect versus sodium fluoride varnish on enamel remineralization. Materials and methods:Thirty-five lower first premolars were alienated for five groups of seven speciemens each. Group I (control): no treatent was applied. The remaining samples were exposed to demineralization. After initiation of lesions, samples were subdivided into four equal groups: Group II: examined immediately. Group III: No treatment was applied. GroupIV: (fluoride group). GroupV: (Curodont Group). Specimens were preserved in artificial saliva. Examination was done after one month using SEM, EDXA and polarized light microscope.Results: EDXA results of Calcium values showed control and Curodont groups had significantly higher values than artificial saliva and demineralized groups. The phosphorous values showed control, artificial saliva, fluoride and Curodont groups had significantly higher values when compared to demineralized group. The Calcium/Phosphorous ratio showed Curodont to have significantly higher value than demineralized and artificial saliva groups. Polarized light microscope results confirmed the SEM-EDXA findings. Conclusion:Self assembling peptide P11-4 was able to remineralize enamel carious lesions more efficiently than sodium fluoride varnish.