The effect of shrimp shell waste on ruminal bacteria and performance of lambs

Cobos, M.A.; Garcia, Lorena; González, S.; Bárcena, J. R.; Hernández, D.; Pérez-Sato, Marcos

doi:10.1016/s0377-8401(01)00319-4

Cited by 13 publications

(9 citation statements)

References 17 publications

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“…Particle-free fluid (1 mL/tube) from the flask was anaerobically transferred to glass tubes (18 x 150 mm) with butyl rubber stoppers. The tubes contained 9 mL of an anaerobic buffer (9 mL/tube; Cobos et al, 2002) and 100 mg of the diet (as DM). Twenty four roll tubes with the diet (100 mg) and ruminal fluid plus buffer (9 mL) were incubated for 24, 48 and 72 h at 39 °C.…”

Section: Methodsmentioning

confidence: 99%

“…Twenty four roll tubes with the diet (100 mg) and ruminal fluid plus buffer (9 mL) were incubated for 24, 48 and 72 h at 39 °C. After the incubation, a 1 mL sample was collected, and serial 10-fold dilutions in an anaerobic mineral solution (Cobos et al, 2002) were prepared. The Hungate (1969) anaerobic technique was used to prepare the media and to cultivate cellulolytic bacteria in the roll-tubes (triplicate) with the complete medium described by Cobos et al (2002).…”

Section: Methodsmentioning

confidence: 99%

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Influence of supplemental aminooligosaccharides on <i>in vitro</i> disappearance of diets for dairy cattle and its effects on milk yield

Sánchez¹,

Pinos-Rodríguez²,

González³

et al. 2011

SA J. An. Sci.

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Two experiments were conducted to evaluate the influence of supplemental aminooligosaccharides (AOS) on the disappearance and fermentation of diets for lactating cows, and its effects on milk yield and quality. In the first study, diets containing 0 or 0.2% AOS (dry matter basis) were incubated for 24, 48 and 72 h with ruminal fluid to evaluate the in vitro dry matter disappearance (IVDMD) and fermentation characteristics. The addition of AOS improved IVDMD of diets, induced higher total bacterial and cellulolytic bacterial counts and decreased the number of bacteria that produce lactate compared to the control. At 24 h incubation, AOS increased volatile fatty acid concentrations and the molar proportion of propionate, though at 48 and 72 h incubation AOS did not affect the fermentation characteristics of the diets. In a further experiment, milk yield and milk quality were evaluated using 50 Holstein dairy cows (193 days in milk). Cows were randomly assigned to the diets tested in the in vitro study. Feed intake was not modified by AOS. Milk yield of cows fed AOS was significantly higher than that of cows not supplemented. Because AOS tended to decrease the percentage of fat in milk, fat and solid-corrected milk content were similar for the two treatments. Percentage and yield of non-fat solids, protein and lactose, and the somatic cell count in milk were not affected by AOS. The milk urea-N concentration of the cows fed AOS was lower than of those not supplemented. Supplementing AOS enhanced the disappearance and fermentation characteristics of the diet. Further studies with cows earlier in lactation are necessary to evaluate the effects of AOS on milk yield and milk components.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Influence of supplemental aminooligosaccharides on <i>in vitro</i> disappearance of diets for dairy cattle and its effects on milk yield

Sánchez¹,

Pinos-Rodríguez²,

González³

et al. 2011

SA J. An. Sci.

View full text Add to dashboard Cite

show abstract

“…Then pH was measured and a I-ml sample was collected, and serial 10-fold dilution in an anaerobic mineral solution (Cobos et al, 2002) was prepared. The Hungate ( 1969) anaerobic technique was used to prepare medium and to cultivate cellulolytic bacteria in roll-tubes (triplicate) with the complete medium described by Cobos et al (2002). Dilutions of lo6 to lo9 were used to cultivate total cellulolytic bacteria.…”

Section: Methodsmentioning

confidence: 99%

Influence of Yeast Products on Rumen Microorganisms,In vitroDegradation and Fermentation of a Diet for Steers

Cobos

Valencia

Pinos-Rodríguez

et al. 2010

Journal of Applied Animal Research

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“…After each incubation time, residual content was filtered by vacuum filtration using a filter paper (Whatman No 541), dried at 608C for 24 h and weighted to calculate the degraded material. Then pH was measured and a 1 mL sample was collected, and a serial 10-fold dilution in an anaerobic mineral solution (Cobos et al 2002) was prepared. The Hungate (1969) anaerobic techniques were used to prepare media and to cultivate total bacteria, cellulolytic bacteria, and lactic acid producer bacteria, in roll-tubes (triplicate) with the complete medium described by Cobos et al (2002).…”

Section: In Vitro Assaymentioning

confidence: 99%

“…Then pH was measured and a 1 mL sample was collected, and a serial 10-fold dilution in an anaerobic mineral solution (Cobos et al 2002) was prepared. The Hungate (1969) anaerobic techniques were used to prepare media and to cultivate total bacteria, cellulolytic bacteria, and lactic acid producer bacteria, in roll-tubes (triplicate) with the complete medium described by Cobos et al (2002). Rumen bacteria were estimated by the most probable number method (Harrigan and McCance 1979) with 95% confidence intervals.…”

Section: In Vitro Assaymentioning

confidence: 99%

Effects of an amino-oligosaccharide on diet degradation and milk production in dairy cows

Gonzalez-Luna

Pinos-Rodríguez

Muñoz

et al. 2011

Journal of Applied Animal Research

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The objective of this study was to evaluate the influence of an amino-oligosaccharide (AAOS) on milk production and in vitro dry matter degradation (IVDMD) of a total mixed ratio (50:50 forage:concentrate) for lactating dairy cows. Treatments (diets) were control (0 g AAOS) and AAOS (0.17% of diet, DM basis). In vitro incubations were carried out at 12, 24, 48 and 70 h. Sixty multiparous Jersey cows (8197 days in milk) were randomly assigned to the two treatments. Milk production and milk components were evaluated during 42 days. The IVDMD was reduced by AAOS only at 12 h incubation. Cows fed AAOS showed similar dry matter intake, milk yield, milk fat, milk non-fat solids and milk lactose as compared to control. However, AAOS increased milk protein percentage and decreased milk urea-nitrogen, as compared to control.

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The effect of shrimp shell waste on ruminal bacteria and performance of lambs

Cited by 13 publications

References 17 publications

Influence of supplemental aminooligosaccharides on <i>in vitro</i> disappearance of diets for dairy cattle and its effects on milk yield

Influence of supplemental aminooligosaccharides on <i>in vitro</i> disappearance of diets for dairy cattle and its effects on milk yield

Influence of Yeast Products on Rumen Microorganisms,In vitroDegradation and Fermentation of a Diet for Steers

Effects of an amino-oligosaccharide on diet degradation and milk production in dairy cows

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