The Effect of Single Type Black Garlic (Allium Sativum L.) Extract on the Cell of Langerhans Islets and the Kidney Tubular Microscopy in Male Wistar Rats (Rattus Norvegicus) Models of Diabetes Mellitus

Prihanti, Gita Sekar; Faradilla, Alifa; Rahman, M M

doi:10.31838/srp.2020.6.46

Cited by 2 publications

(1 citation statement)

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“…A garlic-stimulated pancreatic insulin secretagogue pathway is not clear, as stated in a very recent and comprehensive review by Sanie-Jahromi et al [16]. Previous studies have gone as far as reporting that garlic augments circulatory insulin concentration [13,53], elevates pancreatic insulin histochemical detection [10,54], and increases non-insulin-related gene expressions [16]. In the current study, GE was found to increase the transcription of insulin mRNA only in the pancreas but neither the bile duct nor the liver of DR-GE.…”

Section: Discussionmentioning

confidence: 99%

Among Other Tissues, Short-Term Garlic Oral Treatment Incrementally Improves Indicants of Only Pancreatic Islets of Langerhans Histology and Insulin mRNA Transcription and Synthesis in Diabetic Rats

Al-Adsani,

Al-Qattan

2024

Biology

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Background: The source, mRNA transcription, and synthesis of insulin in the pancreas, in addition to the bile duct and liver, in streptozotocin (STZ)-induced diabetic rats (DR) in response to garlic oral treatment are not yet clear. Objective: This study investigated the accumulative effects of continued garlic oral treatment on changes in the pancreas, bile duct, and liver with regards to: 1—Insulin mRNA transcription, synthesis, and concentration in relation to changes in serum insulin (SI); 2—Insulinogenic cells insulin intensity and distribution, proliferation, and morphology. Method: Fasting blood glucose (FBG) and insulin concentration in serum and pancreas (PI) and sources and mRNA transcription in the pancreas, bile duct, and liver in normal rats given normal saline (NR-NS) and DR given either NS (DR-NS) or garlic extract (DR-GE) before and after 1, 4, and 8 weeks of oral treatment were examined. Results: Compared to NR-NS, DR-NS showed a significant increase in FBG and reductions in SI and PI and deterioration in islets histology, associated pancreatic insulin numerical intensities, and mRNA transcription. However, compared to DR-NS, the targeted biochemical, histological, and genetic variables of DR-GE were significantly and incrementally improved as garlic treatment continued. Insulin or its indicators were not detected either in the bile duct or the liver in DR-GE. Conclusions: 8 weeks of garlic oral treatment is enough to incrementally restore only pancreatic islets of Langerhans insulin intensity and insulinogenic cells proliferation, morphology, and distribution. These indices were associated with enhanced pancreatic insulin mRNA transcription and synthesis. Eight weeks of garlic treatment were not enough to stimulate insulinogenesis in either the bile duct or the liver.

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