The effect of super-oxidized water on the tissues of uterus and ovary:  An experimental rat study

Aras, Abbas; Karaman, Erbil; Çim, Numan; Yıldırım, Serkan; Kızıltan, Remzi; Yılmaz, Özkan

doi:10.5505/ejm.2017.42714

Cited by 4 publications

(4 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…e macroscopic and microscopic examinations of the groups treated with SOW (single and multiple doses of SOW) did not show signi cant di erences compared to the control group (saline solution). Microscopic examination revealed glandular structures in the uterus and functional follicles in di erent stages of maturation in the ovary, demonstrating that the intraperitoneal infusion of SOW does not produce any signi cant toxicity and complications in the tissues of uterus and ovary [53]. Our in vitro results suggest that OxOral® does not produce cytotoxicity to human gingival broblast cells a er 24 h of treatment; the highest percentage of cytotoxicity was 16.5% in the highest dose (15 ppm).…”

Section: Fmentioning

confidence: 62%

Antimicrobial and Anti-Biofilm Effect of an Electrolyzed Superoxidized Solution at Neutral-pH againstHelicobacter pylori

Lucio-sauceda

Urrutia-Baca

Gómez-Flores

et al. 2019

BioMed Research International

View full text Add to dashboard Cite

The presence of Helicobacter pylori in the oral cavity has been associated to the failure of antimicrobial therapy in patients with gastrointestinal infection and the development of oral diseases. However, it has been reported that the maintenance of good oral hygiene can improve the therapeutic success rates, where the use of mouthwashes with anti-Helicobacter activity would help to achieve it. The aim was to evaluate the antimicrobial activity of OxOral® mouthwash against H. pylori and its effect on biofilm formation. The minimum inhibitory concentration (MIC) of OxOral® (pH = 6.4–7.5, ORP = 650–900 mV) against H. pylori was calculated testing serial dilutions 0.117–15 ppm against 1 × 108 CFU/mL of H. pylori (ATCC® 700824™) by broth microdilution method using 96‐well plates. The H. pylori biofilm formation was determined by the optical density measurement at 600 nm from coverslips stained with 0.1% crystal violet. The gene expression of ureA, luxS, flaA, omp18, and lpxD were analyzed by RT‐qPCR. OxOral® cytotoxicity was evaluated in a human gingival fibroblast cell line by MTT assay. MIC was of 3.75 ppm, with 99.7 ± 7.7% bacterial growth inhibition. In the negative control, the biofilm formation was observed, whereas when bacteria were treated with OxOral® at 0.234, 0.469, and 0.938 ppm, an inhibition of 35.5 ± 0.9%, 89.1 ± 1.2%, and 99.9 ± 5.5% were obtained, respectively. The gene expression analysis showed that flaA, omp18, and lpxD genes were down‐regulated with OxOral® compared with control (p<0.05). Low cytotoxicity of 16.5 ± 7.6% was observed at the highest dose (15 ppm); no significant differences were observed from 15 to 0.469 ppm compared to the control of untreated cells (p>0.05). Our results reveal an important anti-Helicobacter activity of OxOral® and open the possibility of its therapeutic use new studies, which would increase the success rate of conventional therapies against H. pylori.

show abstract

Section: Fmentioning

confidence: 62%

Antimicrobial and Anti-Biofilm Effect of an Electrolyzed Superoxidized Solution at Neutral-pH againstHelicobacter pylori

Lucio-sauceda

Urrutia-Baca

Gómez-Flores

et al. 2019

BioMed Research International

View full text Add to dashboard Cite

show abstract

“…34 In the present study, in vitro wound healing activity of 10 fold-diluted StAEW and CEW were noteworthy compared to the control group within 24 hours of exposure. Since HOCl present in EW is a well-documented microbicidal inorganic compound and part of innate immunity in the living system, several studies have evaluated its efficacy on surgical wounds 20,35 and burn-typed wounds. 17,18 As the main active ingredient of “Dakin solution,” 36 HOCl and its contribution to wound healing have been focused on its antimicrobial effect, thus it has been suggested as a preventive agent against infection.…”

Section: Discussionmentioning

confidence: 99%

Toxicity, mutagenicity and stability assessment of simply produced electrolyzed water as a wound healing agent in vitro

et al. 2020

View full text Add to dashboard Cite

Over the last decade, electrolyzed water (EW) produced by salt and tap water has gained importance due to its antimicrobial effects. Regarding to chlorine-based compounds, EW also used in post-harvest safety of food processing and sterilization of surfaces. The latest studies suggested that EW might act as wound healing agent due to anti-infective and cell proliferative properties. In this study, we evaluated acute contact cytotoxicity in L929 mice fibroblast cells and wound healing activity of EWs in vitro. In addition, mutagenic activity was evaluated by Ames test with and without metabolic activation by S9 fraction and the stability profile of freshly prepared EWs has been followed up. According to the results, strong acid (StAEW) and mixed EW (MEW) showed dose-dependent cytotoxicity due to possible high HOCl concentration, while slightly acidic and catholyte EW (CEW) were not cytotoxic even applied directly for 30 sec. Further, StAEW and CEW showed a significant increase in L929 cell migration in scratch assay. Likewise, with/ without metabolic activation, neither of EWs had shown mutagenic profile in TA 98 and TA100 strains of Salmonella typhimurium. Follow-up of ORP (oxidation-reduction potential), pH and FCC (free chlorine concentration) showed that temperature and light were important storage conditions to maintain a stable profile particularly for ORP and FCC, which are the most important indicators for biological activity of EW. According to the present findings, it can be suggested that particularly StAEW, may represent a valuable wound healing agent with an achievable, economical and easy production system when stored under proper conditions.

show abstract

“…7 Water is reduced to oxygen, ozone, and other reactive species; however, the primary ingredient formed during this process is hypochlorite and hypochlorous. 8 In the past 25 years, super-oxidized solutions have been potent antimicrobial agents and disinfectants through oxidative damage. 9 Electrolyzed water contains a mixture of inorganic oxidants, such as hypochlorous acid (HClO), hypochlorous acidic ion (ClO -), chlorine (Cl 2 ), hydroxide (OH), and ozone (O 3 ), which are effectively inactivating a variety of microorganisms responsible for endodontic infections.…”

Section: Introductionmentioning

confidence: 99%

Effects of storage conditions on superoxidised water

Sen,

Huat,

Andrianto

2023

MOJFPT

View full text Add to dashboard Cite

Superoxidised water (SOW) in the food industry has several advantages over traditional chemical disinfectants. SOW is non-toxic and environmentally friendly and does not leave any harmful by-products. In addition, SOW is easy to use and can be applied to various food surfaces. SOW has been used to avoid the food industry's biological pollution caused by food-borne bacteria pathogens with recognized as a short producing time and evident in the sterilization process, SOW is highly recommended for its eco-friendly ability. Chlorine-containing solutions such as SOW are used for broad disinfection purposes, including in the food industry. Still, their effectiveness and stabilities often need to be investigated as residues such as chlorine in SOW are not stable. The efficacy of SOW can be easily affected by several factors, including pH, temperature, and organic matter content. In this study, the two different storage conditions influenced by the light exploration were used to investigate the light condition that directly impacts the chlorine level in SOW. Light intensity plays an important role in determining chlorine loss from SOW. Chlorine dissipation rates in the light condition are higher than in the dark condition. With the initial 40+0.00 mg/L of chlorine, the chlorine level was decreased as plastic bottle/light > glass bottle/light > plastic bottle/without light> glass bottle/without light. They show that the change in chlorine level is significantly influenced by the type of bottle and the environmental condition, such as light intensity, in this study. The results obtained in this study validate the practice of SOW’s storage condition to slow down chlorine's bulk decay when applied in various industries, such as the food industry.

show abstract

The effect of super-oxidized water on the tissues of uterus and ovary: An experimental rat study

Cited by 4 publications

References 8 publications

Antimicrobial and Anti-Biofilm Effect of an Electrolyzed Superoxidized Solution at Neutral-pH againstHelicobacter pylori

Antimicrobial and Anti-Biofilm Effect of an Electrolyzed Superoxidized Solution at Neutral-pH againstHelicobacter pylori

Toxicity, mutagenicity and stability assessment of simply produced electrolyzed water as a wound healing agent in vitro

Effects of storage conditions on superoxidised water

Contact Info

Product

Resources

About