The Effect of TCDD Dioxin on the Rat Liver in Biochemical and Histological Assessment

Czepiel, Jacek; Biesiada, Grażyna; Gajda, Mariusz; Szczepański, Wojciech; Szypuła, Kinga; Dąbrowski, Zbigniew; Mach, Tomasz

doi:10.3409/fb58_1-2.85-90

Cited by 23 publications

(33 citation statements)

References 19 publications

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“…Besides, our study demonstrated that TCDD treatment caused severe histological damages (numerous apoptotic cells and vacuolated hepatocytes) in the liver tissues of rats. These results are in agreement with some previous studies (Patterson et al,2003; Czepiel et al,2010). From our results, it is clear that TCDD treatment at doses of 2 μg/kg/week for 60 days induced lipid peroxidation, histological damage, and reduced antioxidant system activity in rat liver.…”

Section: Discussionsupporting

confidence: 94%

Effects of quercetin and chrysin on 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin induced hepatotoxicity in rats

Çiftçi

Vardı

Özdemır

2011

Environmental Toxicology

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The objective of current study is to investigate the effects of the administration of chrysin (CH) and quercetin (Q) on rat liver in which oxidative and histological damage had been induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Rats were randomly divided into six equal groups. TCDD was orally administered at the dose of 2 μg/kg/week, and Q and CH were orally administered at the doses of 20 mg/kg day and 50 mg/kg/day, respectively, by gavages dissolved in corn oil. The liver samples to be analyzed for the determination of oxidative and histological alternations were taken from rats at 60 days. The results indicated that although 2,3,7,8-TCDD significantly induced (P ≤ 0.01) lipid peroxidation (increase of MDA levels), it positively affected oxidant/antioxidant system (a decline in the levels of GSH, CAT, GSH-Px, and CuZn-SOD) in rats significantly. The histological changes observed in the liver correlated with the biochemical findings. However, these effects of TCDD on oxidative and histological changes were eliminated by Q and CH treatment. In conclusion, TCDD caused an adverse effect on rat's liver. When Q and CH were given together with TCDD, they prevented hepatotoxicty induced by TCDD. Thus, it is thought that Q and CH may be useful as a new category of anti-TCDD toxicity agent.

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Section: Discussionsupporting

confidence: 94%

Effects of quercetin and chrysin on 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin induced hepatotoxicity in rats

Çiftçi

Vardı

Özdemır

2011

Environmental Toxicology

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“…It has been reported that the pathological effects of TCDD in hepatic tissue may lead to the disruption in the functional integrity of hepatocytes (Czepiel et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Propolis alleviates 2,3,7,8-Tetrachlorodibenzo-p-dioxin-induced histological changes, oxidative stress and DNA damage in rat liver

et al. 2012

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2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) induces hepatic damage. Propolis exhibits antioxidant properties and several studies suggest that supplementations with antioxidants can influence hepatotoxicity. Therefore, the aim of the current study was to explore the effectiveness of propolis in alleviating the toxicity of TCDD in the liver of rats. Animals were divided into six groups, namely, TCDD (0.75 and 8 µg/kg body weight (bw)), propolis (50 mg/kg bw), TCDD (0.75 and 8 µg) plus propolis (50 mg/kg bw), and control, respectively. Rats were intraperitoneally administered with their respective doses daily for 21 days. In rats that received a high dose of TCDD, the antioxidant enzymes were significantly decreased and the serious pathological findings were established. Also, the rate of hepatocyte micronucleus (HMN) was increased after treating with TCDD. The reactions of enzymes in control and low-dose group were weak. The frequencies of HMN and liver histology were similar to both the groups. The presence of propolis with TCDD alleviated its pathological effects in hepatic tissue. Propolis also prevented the suppression of antioxidant enzymes in the livers of animals exposed to TCDD and displayed a strong protective effect against HMN. It can be concluded that propolis has beneficial influences and was able to antagonize TCDD toxicity in the liver.

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“…Consistent with our finding, MTT assay demonstrated that the viabilities of human adrenocortical, pancreatic and mammary cells were significantly decreased after TCDD treatment (McDougal et al 1997;Bradshaw et al 2002;Koliopanos et al 2002;Andersson et al 2005. It is reported that the detrimental effects in hepatic tissue of TCDD may lead to disruption in the functional integrity of hepatocytes (Sakamoto et al 1995;Boverhof et al 2006;Czepiel et al 2010;Kopec et al 2010). Thus, TCDD is known to be responsible for the carcinogenic effects associated with oxidative DNA damage (Hung et al 2006).…”

Section: Discussionmentioning

confidence: 99%

Eicosapentaenoic acid protects against 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced hepatic toxicity in cultured rat hepatocytes

et al. 2011

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2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a persistent and ubiquitous environmental contaminant. The health impact of TCDD exposure is of great concern to the general public. Recent reports have implied that eicosapentaenoic acid (EPA) might be a potential chemopreventive agent and influence hepatotoxicity. The aim of the current study was to explore the effectiveness of EPA in alleviating the toxicity of TCDD on primary cultured rat hepatocytes. EPA (5, 10 and 20 lM) was added to cultures alone or simultaneously with TCDD (5 and 10 lM). Rat hepatocytes were treated with TCDD and EPA for 48 h, and then cytotoxicity was detected by [3-(4,5-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide] (MTT) assay and lactate dehydrogenase (LDH) release, while total antioxidant capacity (TAC) and total oxidative stress (TOS) levels were determined to evaluate the oxidative injury. The DNA damage was also analyzed by liver micronucleus assay (LMN) and 8-oxo-2-deoxyguanosine (8-OH-dG). The results of MTT and LDH assays showed that TCDD but not EPA decreased cell viability. TCDD also increased TOS level and significantly decreased TAC level in rat hepatocytes in a clear dose dependent manner. On the basis of increasing doses, the dioxin caused significant increases of micronucleated hepatocytes (MNHEPs) and 8-OH-dG as compared to control culture. Whereas, in cultures treated with EPA alone, TOS level did not change and the level of TAC significantly increased. The presence of EPA with TCDD minimized the toxic effects of the dioxin on primary hepatocytes cultures. Noteworthy, EPA has a protective effect against TCDD-mediated DNA damages.

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The Effect of TCDD Dioxin on the Rat Liver in Biochemical and Histological Assessment

Cited by 23 publications

References 19 publications

Effects of quercetin and chrysin on 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin induced hepatotoxicity in rats

Effects of quercetin and chrysin on 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin induced hepatotoxicity in rats

Propolis alleviates 2,3,7,8-Tetrachlorodibenzo-p-dioxin-induced histological changes, oxidative stress and DNA damage in rat liver

Eicosapentaenoic acid protects against 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced hepatic toxicity in cultured rat hepatocytes

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