The effect of temperature and light intensity on hydrogen gas production by different Rhodopseudomonas capsulata strains

Stevens, P.; Vertonghen, C.; Vos, Paul De; Ley, J. De

doi:10.1007/bf00129054

Cited by 24 publications

(8 citation statements)

References 8 publications

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“…Mais au-delà d'une densité optique égale à 3, la production d'H 2 chute par diminution de l'activité de la nitrogénase liée à une limitation du transfert d'énergie lumineuse dans une culture trop dense et devenue opaque. Une augmentation de la production d'hydrogène allant de pair avec l'augmentation de l'intensité lumineuse a déjà été observée par JOUANNEAU et al (1984) et STEVENS et al (1984). C'est pourquoi, pour maintenir une bonne illumination des cellules, la croissance des bactéries a été limitée par limitation du substrat azoté.…”

Section: Discussionunclassified

Etude de la production d'hydrogène en bioréacteur par une bactérie photosynthétique Rhodobacter capsulatus 1. Photobioréacteur et conditions optimales de production d'hydrogène

Delachapelle¹,

Renaud²,

Vignais³

2005

rseau

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Un réacteur de 10 litres, automatisé pour la culture continue en anaérobiose de bactéries photosynthétiques, a été réalisé et mis au point. Ce réacteur parfaitement agité a été utilisé dans différentes conditions de fonctionnement en système fermé (batch), en système semi-ouvert (atouts de substrats concentrés en discontinu), en système ouvert (chémostat) avec et sans recyclage de la biomasse, afin d'étudier la consommation, par les bactéries, d'un substrat carboné, le lactate. La production d'hydrogène par la bactérie photosynthétique Rhodobacter capsulatus, souche B10, résultant de la dégradation du lactate, est optimale pour des cultures en continu diluées, limitées en source azotée. Ainsi, à un taux de dilution de 0,04 h-1, avec 5 mM glutamate dans le milieu nutritif, la densité bactérienne étant de 2,1 è 660 nm, on a observé une production continue moyenne de 65 ml • h-1• l-1 pendant une période de 200 heures. Pour des concentrations bactériennes élevées, la limitation d'énergie lumineuse entrain une perte d'activité nitrogénase et, de ce fait, une chute de la production d'hydrogène.A photobioreactor was set up to cultivate a photosynthetic bacterium in continuous cultures. The bioreactor was designed so as to 1) allow the capture of light energy by bacteria through a spiral transparent flexible tube placed under the light, in a water bath maintaining the growth temperature at 30 °C; 2) male the suspension of bacteria circulate continuously in the reactor with a volumetric pump to maintain the medium homogeneous; 3) allow degassing of the suspension in a degassing chamber; 4) feed the culture with nutritive media, add neutralizing solution (pH 7) and withdrax aliquots white maintaining constant the volume of the culture; 5) recycle the bacteria by filtration when the bioreactor was used as e closed system (batch).The photosynthetic bacterium was Rhodobacter capsulatus strain B10 is known to lie a good H2 producer [Hillmer and Gest (1977) J. Bacteriol. 129, 724-731]. The bioreactor was run using 10 l of a synthetic medium containing lactate as carbon source and glutamate as nitrogen source. It was studied for its capacity to degrade lactate. Glutamate was the growth-liliting substrate allowing a maximum derepression of nitrogenase, the enzyme catalysing the reduction of protons to H2. The bacterial suspension was continuously circulated in the photoreactor, conceived as a plane light captor of 1 m2, to avoid bacterial self-shading and allow regeneration of ATP by photophosphorylation at high rates. The circuit was tightly closed to avoid air entry, which would prevent H2 production due to respiration of the bacteria.To run it under automated conditions, the bioreactor was equipped with two temperature sensors, two pH electrodes, a water level detector, a manometer and a computer-controlled electric valve. The bioreactor, of the well-mixed type, was used under various working conditions, namely as a closed (batch) system, as a fed-batch system (discontinuous additions of concentrated substrates), and as an ope...

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Section: Discussionunclassified

Etude de la production d'hydrogène en bioréacteur par une bactérie photosynthétique Rhodobacter capsulatus 1. Photobioréacteur et conditions optimales de production d'hydrogène

Delachapelle¹,

Renaud²,

Vignais³

2005

rseau

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“…As the culture conditions were not optimal this yield is probably ira-92 provable. Especially the irradiation, which was reported to influence the conversion efficiency in some strains (Stevens et al 1984) was below light saturation of H2 production at 15000 lx (Steinborn and Oelze 1989 The pH value of the culture medium is of great influence on growth, PHB accumulation and H 2 production. When a short-chain fatty acid such as acetic acid is supplied as its sodium salt to batch cultures the culture medium soon becomes alkaline: growth, PHB accumulation and H 2 evolution responded differently to increasing alkalinization.…”

Section: Discussionmentioning

confidence: 99%

Relationship between the photoproduction of hydrogen and the accumulation of PHB in non-sulphur purple bacteria

Hustede

Steinbüchel

Schlegel

1993

Appl Microbiol Biotechnol

124

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Wild-type cells of Rhodobacter sphaeroidesand Rhodospirillum rubrum strains Ha and S1 as well as mutant cells defective in the synthesis of poly-(3-hydroxybutyric acid) (PHB), were used to study the competition between PHB accumulation and photoproduction of hydrogen for reducing equivalents. Mutants were isolated after transposon (Tn5) or N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis. The PHB-defective mutants of R. sphaeroides lacked PHB synthase activity. In two mutants Tn5 was inserted in the PHB synthase gene. No mutants occurred that lacked the activity of fl-ketothiolase or acetoacetyl-coenzyme A reductase. Pronounced competitive effects occurred only with acetate as the organic substrate. With other organic acids or sugars, which are less readily converted to PHB than acetate, competitive effects were not significant or absent.

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“…For example, the optimum temperature range for hydrogen production by R. sphaeroides was reported to be 30e40 C [41]. In another study [43], six strains of Rb. capsulatus were tested for their ability to produce hydrogen at 20, 25, 30, and 35 C. Three of the tested strains (B100, ST410, and ST407) had better acetate conversion efficiencies at 20 C, two other tested strains (ATCC 23782 and …”

Section: 6mentioning

confidence: 98%

Hydrogen production by a marine photosynthetic bacterium, Rhodovulum sulfidophilum P5, isolated from a shrimp pond

Cai

Wang

2012

International Journal of Hydrogen Energy

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The effect of temperature and light intensity on hydrogen gas production by different Rhodopseudomonas capsulata strains

Cited by 24 publications

References 8 publications

Etude de la production d'hydrogène en bioréacteur par une bactérie photosynthétique Rhodobacter capsulatus 1. Photobioréacteur et conditions optimales de production d'hydrogène

Etude de la production d'hydrogène en bioréacteur par une bactérie photosynthétique Rhodobacter capsulatus 1. Photobioréacteur et conditions optimales de production d'hydrogène

Relationship between the photoproduction of hydrogen and the accumulation of PHB in non-sulphur purple bacteria

Hydrogen production by a marine photosynthetic bacterium, Rhodovulum sulfidophilum P5, isolated from a shrimp pond

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