The Effect of Temperature, Salinity and Irradiance on the Growth of &lt;i&gt;Alexandrium affine&lt;/i&gt; (Dinophyceae) Isolated from Southern Sea of Korea

Kim, Ji Hye; Oh, Seok Jin; Kim, Seok-Yun

doi:10.7837/kosomes.2019.25.2.229

Cited by 4 publications

(1 citation statement)

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“…These salinity ranges were chosen by considering the tolerance data recorded from field surveys of Alexandrium spp. in Korean coastal waters [ 74 , 75 ]. Each salinity condition was adjusted by adding distilled water or by evaporating filtered seawater in a drying oven at 60 °C.…”

Section: Methodsmentioning

confidence: 99%

Salinity Affects Saxitoxins (STXs) Toxicity in the Dinoflagellate Alexandrium pacificum, with Low Transcription of SXT-Biosynthesis Genes sxtA4 and sxtG

Bui

Kim

Park

et al. 2021

Toxins

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Salinity is an important factor for regulating metabolic processes in aquatic organisms; however, its effects on toxicity and STX biosynthesis gene responses in dinoflagellates require further elucidation. Herein, we evaluated the physiological responses, toxin production, and expression levels of two STX synthesis core genes, sxtA4 and sxtG, in the dinoflagellate Alexandrium pacificum Alex05 under different salinities (20, 25, 30, 35, and 40 psu). Optimal growth was observed at 30 psu (0.12 cell division/d), but cell growth significantly decreased at 20 psu and was irregular at 25 and 40 psu. The cell size increased at lower salinities, with the highest size of 31.5 µm at 20 psu. STXs eq was highest (35.8 fmol/cell) in the exponential phase at 30 psu. GTX4 and C2 were predominant at that time but were replaced by GTX1 and NeoSTX in the stationary phase. However, sxtA4 and sxtG mRNAs were induced, and their patterns were similar in all tested conditions. PCA showed that gene transcriptional levels were not correlated with toxin contents and salinity. These results suggest that A. pacificum may produce the highest amount of toxins at optimal salinity, but sxtA4 and sxtG may be only minimally affected by salinity, even under high salinity stress.

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Section: Methodsmentioning

confidence: 99%