The Effect of Testosterone Replacement Therapy on Bladder Functions and Histology in Orchiectomized Mature Male Rats

Tek, Mesut; Ballı, Ebru; Çimen, Burak; Efesoy, Ozan; Oguz, Izzet; Çayan, Selahíttín

doi:10.1016/j.urology.2009.08.016

Cited by 42 publications

(32 citation statements)

References 22 publications

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“…Recent studies showed that TRT in SLOH men with urogenital dysfunction may have a positive role by increasing quantity of smooth muscle fibers that leads to improvement of bladder capacity and function Tek et al 2010;Magari et al 2014). Androgen deficiency induces bladder fibrosis and decreases the bladder maximal volume and compliance which could be effectively prevented by TRT (Gallo et al 2012;Zhang et al 2012;de Barros et al 2013).…”

Section: Discussionmentioning

confidence: 99%

“…Besides its role in improving the metabolic syndrome, obesity parameters and other health-related quality of life (Yassin et al 2014), testosterone replacement therapy (TRT) can improve bladder function and reversing its wall alterations by increasing urinary bladder (UB) smooth muscle in late-onset hypogonadal men with urogenital dysfunction (Tek et al 2010). TRT acts on androgen receptors (AR) which are widely expressed in the bladder, including urothelium, muscularis propria and neurons (Salmi et al 2001;Celayir et al 2002).…”

Section: Introductionmentioning

confidence: 99%

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Effect of testosterone therapy on the urinary bladder in experimental hypogonadism of rats

Abdel-Hamid

Ali

2015

J Mol Hist

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Testosterone (T) deficiency is prevalent particularly in elderly men and lead to physical and sexual morbidities. Although low levels of T are associated with low urinary tract symptoms, the correlation between T deficiency and bladder dysfunction is not clearly identified. The aim of this study was to investigate the effect of high dose testosterone replacement therapy (TRT) on the histological structure of the UB in castrated rats. Twenty-five adult male rats were divided into three groups: control, castrated and castrated + TRT. T was administrated in high dose (100 mg/kg) two intramuscular injections/week for 60 days. UB sections were prepared and stained with H&E, Masson's trichrome and immunohistochemical detection of Cytokeratin 20 (Ck20). All data were morphometrically and statistically analyzed. In castrated group, significant atrophy of the urothelium (P < 0.001) accompanied with widening of the corium were observed. The smooth muscle appeared thin with marked increase in the collagen fibers. On treating the castrated group with TRT, atypical Ck20 expression as well as significant increase in urothelial thickness (P < 0.05) and smooth muscle/collagen ratio (P < 0.001) were detected. In castrated rat model, high dose TRT has a positive effect on the UB smooth muscle rather than the urothelium which acquired atypical patterns.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of testosterone therapy on the urinary bladder in experimental hypogonadism of rats

Abdel-Hamid

Ali

2015

J Mol Hist

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“…The baseline (empty bladder), opening (at first leakage), and peak pressure (maximal pressure during voiding) and the maximal bladder volume were recorded. Bladder compliance (mL/cm H 2 O) was calculated as described previously (Tek et al 2009). …”

Section: Functional Evaluationmentioning

confidence: 99%

“…Yu and colleagues reported that ovariectomy induced a thinner bladder wall and an impaired ultrastructure of detrusor cells and widened spaces between the detrusor muscle fascicles with collagen deposit in female rats, suggesting the importance of steroid hormones in maintaining the integrity of the bladder structure (Yu et al 2009). Tek et al (2009) have demonstrated that androgen deprivation in orchiectomized mature rats leads to decreased bladder capacity and collagen deposit, which could be prevented by androgen replacement treatment. However, the underlying mechanism and intermediated molecule of androgen deficiencyinduced bladder fibrosis and dysfunction remain unknown.…”

mentioning

confidence: 99%

Androgen Deprivation Induces Bladder Histological Abnormalities and Dysfunction via TGF-.BETA. in Orchiectomized Mature Rats

Zhang

Ji-min

et al. 2012

Tohoku J. Exp. Med.

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Symptomatic late-onset hypogonadism, one of the most common elder diseases, is defined as a syndrome associated with a deficiency in serum testosterone. Recent studies have indicated that androgen deficiency in men is also associated with lower urinary tract symptoms and bladder dysfunction. To determine the pathologic consequences of androgen deprivation in bladder histology and function, we addressed the underlying mechanism. Male rats were divided into 4 groups: emasculated rats (EMR), emasculated rats treated with testosterone, emasculated rats treated with anti-transforming growth factor-β (TGF-β) neutralizing antibody, and sham surgery rats. TGF-β is a common profibrotic factor that mediates the pathologic process of fibrosis in multiple organs. Two months later, urodynamic evaluations were employed to determine the bladder function in vivo. And then rats were sacrificed, and the bladder tissues were collected. Histological studies were employed to determine the degree of bladder fibrosis. Real time PCR was used to evaluate the mRNA level of pro-collagen I, a fibrotic marker. We demonstrate here that androgen deficiency induces bladder fibrosis and decreases the bladder maximal volume and compliance. Androgen replacement treatment completely prevented the histological and functional abnormalities induced by androgen deficiency. Subsequently, we identified that androgen deprivation induced the induction of TGF-β mRNA level. Importantly, treatment with anti-TGF-β antibody abolished androgen deprivation-induced bladder fibrosis and dysfunction. Our study reveals an essential role of TGF-β in the pathogenesis of androgen deprivation-induced bladder fibrosis and dysfunction and offers a potential target for prevention and treatment of bladder dysfunction associated with androgen deficiency.

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“…Recent studies have suggested that changes in male hormones and various lower urinary tract symptoms (LUTS) are related to aging [1][2][3][4][5]. Androgen may play a critical role in male LUTS, mainly through its effects on prostate growth and function.…”

Section: Introductionmentioning

confidence: 99%

Influence of castration on bladder blood flow and function during the rapid phase of androgen deprivation

Magari

Shibata

Arai

et al. 2012

Scandinavian Journal of Urology

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The Effect of Testosterone Replacement Therapy on Bladder Functions and Histology in Orchiectomized Mature Male Rats

Cited by 42 publications

References 22 publications

Effect of testosterone therapy on the urinary bladder in experimental hypogonadism of rats

Effect of testosterone therapy on the urinary bladder in experimental hypogonadism of rats

Androgen Deprivation Induces Bladder Histological Abnormalities and Dysfunction via TGF-.BETA. in Orchiectomized Mature Rats

Influence of castration on bladder blood flow and function during the rapid phase of androgen deprivation

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